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Pectic oligosaccharide, preparation method thereof and application of AGEs

A technology of pectin oligosaccharides and pectin, applied in the application of AGEs inhibitors, pectin oligosaccharides and its preparation field, can solve the problem of little research on anti-glycation activity, so as to inhibit the formation of AGEs, high AGEs Inhibition Rate, Effect of Hypoglycemic Immunity

Inactive Publication Date: 2019-01-18
LIAONING UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, at present, the research on the biological activity of pectin and its oligosaccharides is mostly focused on its probiotic and anti-oxidative functions, but there is little research on its anti-glycation activity
Especially the in vitro anti-glycation activity of pectin oligosaccharides and the research on the relationship with molecular weight have not been reported so far.

Method used

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  • Pectic oligosaccharide, preparation method thereof and application of AGEs
  • Pectic oligosaccharide, preparation method thereof and application of AGEs
  • Pectic oligosaccharide, preparation method thereof and application of AGEs

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Embodiment

[0023] (1) Preparation of pectin oligosaccharides

[0024] 1. Separation and extraction of hawthorn pectin

[0025] Dry the hawthorn and grind it into powder. Take a certain amount of dry hawthorn powder, add distilled water according to the ratio of material to liquid 1:20 (g / mL), stir and extract at 90°C for 4 hours, then centrifuge (4000rpm, 10min) to collect the supernatant, and use 3 times the volume of absolute ethanol alcohol Sink and centrifuge to collect the precipitate, dry to obtain powdered pectin, and set aside.

[0026] 2. Preparation of pectin oligosaccharides

[0027] Prepare 0.02mol / L acetate buffer solution, dissolve powdered pectin in acetate buffer solution to obtain a pectin solution with a concentration of 2mg / mL, add pectinase to make the final concentration of pectinase reach 0.2U / mL , enzymatically hydrolyzed at 40°C for 4h, and then boiled at 100°C for 10min to inactivate the enzyme activity to obtain the enzymatic hydrolyzate.

[0028] 3. Separat...

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Abstract

The invention discloses pectic oligosaccharide, a preparation method thereof and application of an AGEs inhibitor. The preparation method comprises the following steps: adequately stirring the raw material, namely haw powder at 90 DEG C for 4 hours by virtue of a hot-water method, and separating to extract haw pectin; degrading pectin by virtue of pectinase, and collecting products with molecularweights of 1kDa-3kDa from enzymatic hydrolysate respectively through 1kDa-3kDa polysulfone membranes. The pectic oligosaccharide with a medium molecular weight has the advantages that the raw materialis easily available, the operation is simple, the inhibition rate to the generation of AGEs is high, and the pectic oligosaccharide is safe and non-toxic and low in cost; and the pectic oligosaccharide, as a dietary fiber has probiotics activity and multiple biological activities of resisting oxidation activity, reducing blood fat and the like.

Description

technical field [0001] The invention belongs to the field of preparation and application of biologically active natural products, and in particular relates to a medium-molecular-weight pectin oligosaccharide, a preparation method thereof, and an application as an AGEs inhibitor. Background technique [0002] Glycosylation end products (AGEs) are a class of stable products formed by the amino terminals of carbohydrates, proteins, amino acids and other substances in Maillard reactions in food or biological systems without enzymatic reactions, including N-carboxylates There are nearly 40 kinds of methyllysine (CML), N-carboxyethyllysine (CEL), pentosinoside, pyrrolidine, pyrrolealdehyde, glyoxal, and crosslinking. In addition, lipid peroxidation products and reducing sugar autooxidative degradation products can also undergo complex changes to produce AGEs. [0003] Food-borne AGEs are an important source of AGEs in the human body. The long-term accumulation of AGEs in the bod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20A23L33/21C12P19/00C12P19/04C12P19/12
CPCA23V2002/00A23L5/27A23L33/21C12P19/00C12P19/04C12P19/12A23V2200/3202A23V2200/3262A23V2200/328
Inventor 祝儒刚张鑫雨侯玉婷
Owner LIAONING UNIVERSITY
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