Screening method and application of Henan gamecock genome SNP molecular markers

A molecular marker and genome technology, applied in the field of genetics, can solve the problems of low accuracy and high cost of Henan gamecock genetic differentiation research, and achieve the effect of low cost, abundant data and high detection accuracy

Active Publication Date: 2022-05-06
JIANGSU INST OF POULTRY SCI
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Problems solved by technology

[0008] The purpose of the present invention is to provide a screening method for Henan gamecock genome SNP molecular markers, which alleviates the high cost and low accuracy of the research on Henan gamecock genetic differentiation caused by the lack of molecular markers capable of evaluating the genetic diversity of Henan gamecocks in the current prior art technical issues

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  • Screening method and application of Henan gamecock genome SNP molecular markers
  • Screening method and application of Henan gamecock genome SNP molecular markers
  • Screening method and application of Henan gamecock genome SNP molecular markers

Examples

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Embodiment 1

[0062] Example 1 Screening of Henan gamecock genome SNP molecular markers

[0063] (a) Blood sample collection: Henan gamecock and 18 local chicken breeds of the control group (Langshan chicken, Anyiwa gray chicken, Gushi chicken, Xianju chicken, white-eared yellow chicken, Dongxiang green-shell chicken, Jinhu Wu Feng chicken, Wenchang chicken, Daweishan micro chicken, Tibetan chicken, camellia chicken, ladle chicken, Huiyang bearded chicken, big bone chicken, Xiaoshan chicken, Luyuan chicken, Bian chicken and Beijing oil chicken) and 2 introduced chicken breeds (hidden White-feathered broiler chicken and Anka red chicken) come from the national-level local chicken breed gene bank of Jiangsu Institute of Poultry Science. Each group selects 30 individuals (10 males and 20 females) according to the family line, and there is no relationship between individuals. 1 mL-1.5 mL of blood was collected from the wing vein, anticoagulated with sodium citrate (ACD), and stored at -20°C for...

Embodiment 2

[0079] Example 2 Application of Molecular Markers in Gamecocks in Henan

[0080] (a) Individual blood collection of Henan cockfighting preservation population: collect 0.5-1.0ml of venous whole blood from male and female chicken wings of the preservation population (no less than 10 roosters and no less than 20 hens are randomly selected), and inject the blood containing 2μL 0.5mol / L Na 2 EDTA anticoagulant in an enzyme-free tube, and then store the enzyme-free tube at 4°C for future use.

[0081] (b) DNA extraction and quality inspection: suck out 0.2-0.3ml of male and hen individual blood stored in enzyme-free tubes at room temperature, and extract individual blood DNA by conventional animal peripheral blood phenol-phenol extraction; The DNA integrity was analyzed by agarose gel electrophoresis, and the DNA purity was detected by a spectrophotometer; the qualified samples were stored at -80°C for use in SNP detection.

[0082] (c) SNP marker detection of Henan gamecock bree...

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Abstract

The invention relates to the field of genetics, in particular, it provides a screening method of Henan gamecock genome SNP molecular marker and its application. The screening method detects through high-throughput sequencing, calculates genetic statistics, analyzes the genetic differences between Henan gamecock and other chicken breeds, and screens out Henan gamecock SNP molecular markers. This screening method alleviates the technical problems of high cost and low accuracy of genetic differentiation research caused by the lack of efficient molecular biology methods and genetic markers in the identification of genetic differentiation of gamecocks in Henan, and achieves the technical effect of efficient and rapid screening of molecular markers in Henan gamecocks , the selected genes of Henan Gamecock were screened and used as molecular markers to identify the germplasm of Henan Gamecock, with low cost, abundant data and high detection accuracy. The application of the SNP molecular markers for the identification of Henan gamecock genome has alleviated the technical problem of lack of efficient molecular biology markers in the study of genetic differentiation of Henan gamecock.

Description

technical field [0001] The invention relates to the field of genetics, in particular to a screening method for SNP molecular markers in Henan gamecock genome and an application thereof. Background technique [0002] Henan Game Chicken, also known as Central Plains Game Chicken, originates from Kaifeng City, Henan Province. Henan gamecock has a long history of breeding. During the long-term selection and domestication process, it has formed a completely different appearance from other local chicken breeds. It has strong fighting and fighting ability. It is an extremely valuable genetic resource in my country's poultry bank. [0003] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) mainly refers to the DNA sequence polymorphism caused by the variation of a single nucleotide at the genome level, including transitions, transversions, deletions and insertions. Genetic markers have a large number and rich polymorphisms. Theoretically, each SNP site can have 4 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6869
CPCC12Q1/6869C12Q1/6888C12Q2600/156C12Q2600/124C12Q2535/122
Inventor 殷建玫朱云芬韩威李国辉张会永朱静苏一军盛中伟沈海玉
Owner JIANGSU INST OF POULTRY SCI
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