Quick detecting method of bovine leukemia virus

Abstract

The invention relates to a quick detecting method of bovine leukemia virus, which directly takes blood as a detecting sample, does not need special treatment, and is convenient to collect, store and transport. Primers are designed to amplify LTR of bovine leukemia virus (BLV) provirus, which ensured the accuracy and stability of the method. Based on the nested PCR detecting method, the quick detecting method of bovine leukemia virus can detect bovine leukemia virus (BLV) in blood samples quickly, and its sensitivity is 1 fg DNA template, which is 100 times higher than that of PCR. The quick detecting method of bovine leukemia virus provides a quick and accurate diagnostic tool for the clinical diagnosis of bovine leukemia, and has important practical significance for the epidemiological investigation and purification of bovine leukemia.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows us direct use of whole blood instead of specific parts like cells or plasma from cattle infected with BLV. It simplifies the process by eliminating any extra steps needed before testing it. By performing this analysis at least one section of the genome containing gene fragments that indicate how severe disease affects sheep's immune system, we aimed towards developing an improved way to accurately identify diseases such as lymphoma caused by Babesia bovis.

Problems solved by technology

Technics: Current diagnoses involving detecting bovidence disorders involve testing animals from different parts of their body through various techniques like histopathologic examination and culture analysis. However these tests require significant resources due to the lengthy duration involved and poor accuracy rates associated with current methodologies. Additionally, existing methods often lack specificity towards certain types of pathogens making them hard targets against other strains causing cross resistance issues during research efforts aiming at developing new therapies targeting those resistant forms of bacterial growth.

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