A culture medium for improving the active ingredient of Cordyceps sinensis mycelium
An active ingredient, the technology of Cordyceps sinensis, which is applied in the field of culture medium to improve the active ingredients of Cordyceps sinensis mycelium, can solve the problems of low content of active ingredients, uneven quality of Cordyceps sinensis mycelium, and lack of natural Cordyceps efficacy, etc., to achieve high nutrition value and the effect of shortening the cultivation period
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Embodiment 1
[0042] A preparation method of Cordyceps sinensis mycelium, comprising the following steps:
[0043] (1) Inoculation culture: put 2000mL Erlenmeyer flask into 1000mL medium for increasing the active ingredient of Cordyceps sinensis mycelium, sterilize at 121°C for 30 minutes, cool to 25°C, and inoculate Trichosporum bat moth under aseptic conditions The strains were cultivated on a constant temperature shaker at 20°C until the mycelium concentration was 32wt%, and the shaker speed was 160 rpm to obtain a liquid strain culture solution in an Erlenmeyer flask;
[0044] (2) Seed tank cultivation: 100 L of medium for improving the active ingredient of Cordyceps sinensis mycelium is loaded into a 200 L fermenter, sterilized at 121° C. for 30 minutes, cooled to 25° C., and the liquid strain culture solution of the Erlenmeyer flask Add it into a fermenter, and cultivate it for 10 days at a temperature of 20°C and a stirring speed of 180 rpm. During the fermentation process, the tank ...
Embodiment 2
[0063] A preparation method of Cordyceps sinensis mycelium, comprising the following steps:
[0064] (1) Inoculation culture: put 2000mL Erlenmeyer flask into 1000mL medium for increasing the active ingredient of Cordyceps sinensis mycelium, sterilize at 121°C for 30 minutes, cool to 25°C, and inoculate Trichosporum bat moth under aseptic conditions The strains were cultivated on a constant temperature shaker at 20°C until the mycelium concentration was 32wt%, and the shaker speed was 160 rpm to obtain a liquid strain culture solution in an Erlenmeyer flask;
[0065] (2) Seed tank cultivation: 100 L of medium for improving the active ingredient of Cordyceps sinensis mycelium is loaded into a 200 L fermenter, sterilized at 121° C. for 30 minutes, cooled to 25° C., and the liquid strain culture solution of the Erlenmeyer flask Add it into a fermenter, and cultivate it for 10 days at a temperature of 20°C and a stirring speed of 180 rpm. During the fermentation process, the tank ...
Embodiment 3
[0078] A preparation method of Cordyceps sinensis mycelium, comprising the following steps:
[0079] (1) Inoculation culture: put 2000mL Erlenmeyer flask into 1000mL medium for increasing the active ingredient of Cordyceps sinensis mycelium, sterilize at 121°C for 30 minutes, cool to 25°C, and inoculate Trichosporum bat moth under aseptic conditions The strains were cultivated on a constant temperature shaker at 20°C until the mycelium concentration was 32wt%, and the shaker speed was 160 rpm to obtain a liquid strain culture solution in an Erlenmeyer flask;
[0080] (2) Seed tank cultivation: 100 L of medium for improving the active ingredient of Cordyceps sinensis mycelium is loaded into a 200 L fermenter, sterilized at 121° C. for 30 minutes, cooled to 25° C., and the liquid strain culture solution of the Erlenmeyer flask Add it into a fermenter, and cultivate it for 10 days at a temperature of 20°C and a stirring speed of 180 rpm. During the fermentation process, the tank ...
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