Preparation method and application of a kind of degreasing bacteria agent, its composite dry powder bacteria agent
A kind of oil bacteria agent and bacteria agent technology, which is applied in the preparation of oily rock cuttings, sludge oil removal bacteria agent, and landing oil treatment, can solve the problem of high processing cost and operating cost, excessive power consumption and complicated process of incineration method. problems, to achieve the effect of increased plant beneficial components, good environmental tolerance, and high microbial activity
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Embodiment 1
[0035] Oily sewage and sludge samples were taken from multiple oily sewage and sludge pools in Jiangsu Oilfield; the bacteria in them were enriched and cultured in LB medium, acid-base neutral environment, and 25-30°C, and then separated and purified and screening, two strains of highly active oil-removing bacteria were obtained, namely:
[0036] GWN-1: Sphingobacterium sp. (Latin literary name is Sphingobacterium sp.), preserved in the General Microorganism Collection Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC), preservation date: March 20, 2017, preservation number: CGMCC No. 13902; its sequencing is shown in 1 in the sequence listing.
[0037] GWN-2: Bacillus brevis (Latin literary name is Brevibacillus parabrevis), preserved in the General Microorganism Collection Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC), preservation date: March 20, 2017, preservation number: CGMCC No. 1390...
Embodiment 2
[0041] The difference from Example 1 is that the two bacterial solutions are mixed according to the volume ratio of GWN-1 bacterial solution: GWN-2 bacterial solution = 1:1.9 to obtain a mixed solution.
Embodiment 3
[0043] The two bacterial solutions were mixed according to the volume ratio of GWN-1 bacterial solution: GWN-2 bacterial solution = 1:2.1 to obtain a mixed solution.
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