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Preparation method and application of a kind of degreasing bacteria agent, its composite dry powder bacteria agent

A kind of oil bacteria agent and bacteria agent technology, which is applied in the preparation of oily rock cuttings, sludge oil removal bacteria agent, and landing oil treatment, can solve the problem of high processing cost and operating cost, excessive power consumption and complicated process of incineration method. problems, to achieve the effect of increased plant beneficial components, good environmental tolerance, and high microbial activity

Active Publication Date: 2022-02-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of sludge degreasing treatment, methods such as conditioning and tempering separation technology, incineration technology, solvent extraction oil recovery technology, and coking modification treatment technology are mainly used at home and abroad. However, from the perspective of application effect, the more common problems are: incineration The processing cost and operation cost of the method are high, and the processing process produces SO 2 , CO and dust and other secondary pollution; the solvent method has a long process, complicated process, high solvent price and high treatment cost; the coking method has high investment and complicated process
[0005] Analysis of existing patented technologies: Among the patented technologies of oilfield sludge biological treatment methods, there are related patented technologies for oilfield sludge biological treatment bacteria and methods. .4), add water to mix the sludge water pump stirring cycle to realize oil-water separation, the water content in the mixing is 95% on average, the power consumption is too large, and the oil content of the compost reaches the standard of 0.3% one month after the bacterial agent is mixed in after the reaction; a kind of utilization A method for microbial degradation of oily sludge at a well site (ZL201010550531.X), the oil content of the on-site sludge was degraded from 10.55% to 1.1% after 56 days of treatment, but still could not reach the 0.3% standard; a petroleum-degrading bacterium in oily sludge and its application (ZL201410072228.1), at a temperature of 37 degrees and a salinity of 0.5-5%, the total petroleum hydrocarbon degradation rate of the isolated strain was 39.69% within 30 days. The actual oilfield sludge salinity and treatment temperature are generally difficult to meet this requirement; polluted by crude oil Crude oil degradation agent in soil (ZL20110343690.5), the treatment object does not include the oily sludge produced in the oil-water system of the oilfield production link, it is limited to the soil contaminated by crude oil on the surface, and its treatment effect is 100g / kg to 15g / kg kg, does not meet the 0.3% standard, and is not suitable for the technical field of high water content and oily sludge produced in the process of oil and water treatment in oil field production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Oily sewage and sludge samples were taken from multiple oily sewage and sludge pools in Jiangsu Oilfield; the bacteria in them were enriched and cultured in LB medium, acid-base neutral environment, and 25-30°C, and then separated and purified and screening, two strains of highly active oil-removing bacteria were obtained, namely:

[0036] GWN-1: Sphingobacterium sp. (Latin literary name is Sphingobacterium sp.), preserved in the General Microorganism Collection Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC), preservation date: March 20, 2017, preservation number: CGMCC No. 13902; its sequencing is shown in 1 in the sequence listing.

[0037] GWN-2: Bacillus brevis (Latin literary name is Brevibacillus parabrevis), preserved in the General Microorganism Collection Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC), preservation date: March 20, 2017, preservation number: CGMCC No. 1390...

Embodiment 2

[0041] The difference from Example 1 is that the two bacterial solutions are mixed according to the volume ratio of GWN-1 bacterial solution: GWN-2 bacterial solution = 1:1.9 to obtain a mixed solution.

Embodiment 3

[0043] The two bacterial solutions were mixed according to the volume ratio of GWN-1 bacterial solution: GWN-2 bacterial solution = 1:2.1 to obtain a mixed solution.

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PUM

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Abstract

The invention discloses an oil-removing bacterial agent in the technical field of oily sludge treatment, a preparation method and application of its composite dry powder bacterial agent, and the oil-removing bacterial agent contains two kinds of bacterial liquids of GWN-1 strain and GWN-2 strain The mixed solution, the number of viable bacteria in each bacterial solution is 10 9 ~10 11 each / ml; the two kinds of bacterial liquids are respectively GWN‑1 bacterial liquid by volume ratio: GWN‑2 bacterial liquid=1: (1.9~2.1) to mix; Press filtration and drying obtains a dry powder, which is used in the treatment of oily sludge from oilfields. The composite dry powder bacterial agent is suitable for direct biodegradation of oily sludge in oilfields with high water content, high oil content and high salinity, has good environmental tolerance, and the preparation method has the characteristics of simple operation and low cost, and is suitable for large-scale production and application.

Description

technical field [0001] The invention relates to the preparation and use method of a sludge deoiling bacterial agent, which is especially suitable for the treatment of oily sludge produced in the field of crude oil exploitation and treatment in oilfields, oily cuttings produced in the drilling process, and ground oil, and belongs to oily pollution in oilfields Mud treatment technology field. Background technique [0002] In the production of my country's petrochemical industry, a large amount of oily sludge is produced every year, and the output is still increasing year by year. As a pollutant in the "National Hazardous Waste List", oily sludge must be treated, otherwise it will cause damage to the surrounding environment and production areas. Different degrees of impact, and it is also a waste of resources. Effective separation and recovery of oily components in sludge can not only reduce resource waste, but also a prerequisite for the development of harmless treatment and re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/01C02F101/32C02F103/10
CPCC12N1/20C02F11/02C02F2101/32C02F2103/10
Inventor 郭鹏封苏海李汉周林刚庄建全
Owner CHINA PETROLEUM & CHEM CORP
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