A method for preparing bio-bricks by using microorganisms to induce calcium carbonate deposition
A bio-brick and calcium carbonate technology, which is applied in the field of bio-brick preparation by using microorganisms to induce calcium carbonate deposition, can solve problems such as calcium carbonate deposition and preparation of bio-brick, and achieve the effects of high compressive strength, good environmental tolerance and simple operation.
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Embodiment 1
[0031] A method for preparing bio-bricks by using microorganisms to induce calcium carbonate deposition, comprising the following steps:
[0032] S1. Use culture solution (glucose 20 g / L, ammonium chloride 4.048 g / L, nickel sulfate 2 grains / L, peptone 1 g / L, solvent is water) to separate Bacillus pasteurianus (BNCC 337394) and pseudostrongia Bacillus (BNCC 132711) cultured to 1 × 10 6 cfu / mL, mix the obtained Bacillus pasteurianus bacteria solution and Bacillus pseudostrongis bacteria solution at a volume ratio of 1:1 to obtain a composite bacteria solution;
[0033] S2. Mix the composite bacterial solution with the mixed saline solution (3 mol / L calcium lactate and 3 mol / L magnesium chloride), and 3 mol / L urea solution at a volume ratio of 1:1:1 to obtain a solidified solution;
[0034] S3. Mix the river sand with the above mixture at a material-to-liquid ratio (kg / L) of 1:4 after being screened with 22 meshes, and then add it at a weight ratio of river sand:quicklime=3:1 (...
Embodiment 2
[0037] Compared with Example 1, the difference between Example 2 is that the mixing volume ratio of the Bacillus pasteurian bacteria liquid and the Bacillus pseudofaster bacteria liquid is 1:0.5.
Embodiment 3
[0039] Compared with Example 1, Example 3 differs in that: the mixing volume ratio of the Bacillus pasteurian bacteria liquid and the Bacillus pseudofaster bacteria liquid is 1:1.5.
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