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Four-autoantibody combined detection kit for diagnosing early esophageal squamous cancer and application thereof

An autoantibody and combined detection technology, applied in the field of medical diagnosis, can solve the problems of limited clinical application value, low sensitivity and specificity, and improve the quality of serological screening, high sensitivity and specificity, and promote the cure rate Effect

Active Publication Date: 2019-01-04
FUJIAN PROVINCIAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current research, people are still trying to find more sensitive and specific anti-TAAs autoantibodies for the diagnosis of ESCC. Although anti-TAAs autoantibodies have been reported in the literature, their sensitivity and specificity are low, and their clinical application value is limited.

Method used

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  • Four-autoantibody combined detection kit for diagnosing early esophageal squamous cancer and application thereof
  • Four-autoantibody combined detection kit for diagnosing early esophageal squamous cancer and application thereof
  • Four-autoantibody combined detection kit for diagnosing early esophageal squamous cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Eight kinds of serum autoantibodies are tested for early diagnosis of esophageal squamous cell carcinoma (small sample)

[0031] 1 Materials and methods

[0032] 1.1 Research object

[0033] A total of 150 serum samples were collected from Fujian Provincial Hospital from March 2015 to February 2016, including 50 early ESCC patients, 50 EBL patients, and 50 HC patients. All 50 patients with early ESCC were diagnosed on the basis of endoscopy combined with cytology or histopathology. They did not receive any anti-tumor treatment such as chemotherapy and radiotherapy. The TNM classification was stage 0, I, and II. The 50 cases of EBL patients came from patients who were initially treated in Fujian Provincial Hospital during the same period, including benign esophageal tumors, benign esophageal ulcers, esophageal erosion, and reflux esophagitis. Fifty HC patients were healthy adults from the Physical Examination Center of Fujian Provincial Hospital, which did n...

Embodiment 2

[0043] Example 2: Detection of four kinds of serum autoantibodies on the early diagnosis test of esophageal squamous cell carcinoma (large sample)

[0044] Based on the results of small-sample research, the purpose of this study is to study the value of anti-TP53, HRAS, CTAG1A, and NSG1 antibody detection in the early diagnosis of ESCC in a large sample of 569 cases of serum by constructing an ELISA method. The research cohort includes 409 A training set of 160 cases and a validation set of 160 cases with independent matching.

[0045] 1 Materials and methods

[0046] 1.1 Research object

[0047] A total of 569 serum samples were collected from Fujian Provincial Hospital from February 2016 to June 2018, including 179 early ESCC patients, 180 EBL patients, and 210 HC patients. All 179 patients with early ESCC were diagnosed on the basis of endoscopy combined with cytology or histopathology. They did not receive any anti-tumor treatment such as chemotherapy and radiotherapy. T...

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Abstract

The invention provides a four-autoantibody combined detection kit for diagnosing an early esophageal squamous cancer and application thereof. The four-autoantibody combined detection kit for diagnosing the early esophageal squamous cancer comprises a solid substrate and antigen protein which coats the solid substrate; and the antigen protein comprises TP53, HRAS, CTAG1A and NSG1. The four-autoantibody combined detection kit for diagnosing the early esophageal squamous cancer, provided by the invention, has the advantages that the TP53, the HRAS, the CTAG1A and the NSG1 are firstly adopted as acombination to be used for early diagnosis of ESCC, higher sensibility and higher specificity are realized, the detection kit is conducive to further improvement of serologic screening quality of ESCC early diagnosis, and therefore, the cure rate and survival rate of patients are further promoted.

Description

technical field [0001] The invention belongs to the technical field of medical diagnosis, and in particular relates to a multiple autoantibody combined detection kit for diagnosing early esophageal squamous cell carcinoma based on Luminex xMAP technology and its application. Background technique [0002] Esophageal squamous cell carcinoma (ESCC) is one of the most common upper gastrointestinal malignancies worldwide. As an area with a high incidence of esophageal cancer, China has the highest incidence rate of ESCC in the world, and ranks the fourth cause of cancer death (Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and Mortality worldwide: Sources, methods and major patterns in GLOB ℃ AN2012. Int J Cancer 2015; 136(5):E359-86.). ESCC patients often have no specific clinical symptoms in the early stage and are difficult to be detected. It is difficult to find them, and the five-year survival rate is only 15-25% (PennathurA, Gibs...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 黄毅钱江朱衡郑庆祝李亚东余丽丽吴庆伟郑佳莹
Owner FUJIAN PROVINCIAL HOSPITAL
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