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Method for extracting beta-glucan in oat and oatmeal processing byproducts

An extraction method and technology of glucan, which is applied in the field of refining and processing plant active ingredients, can solve the problem of low purity of β-glucan extraction, achieve the effects of solving protein residues, simplifying the process, and reducing costs

Inactive Publication Date: 2019-01-04
金维他(福建)食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method for extracting β-glucan in oat and oatmeal processing by-products, so as to solve the problem that the extraction purity of β-glucan in the prior art is not high

Method used

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  • Method for extracting beta-glucan in oat and oatmeal processing byproducts
  • Method for extracting beta-glucan in oat and oatmeal processing byproducts
  • Method for extracting beta-glucan in oat and oatmeal processing byproducts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Extraction of oat β-glucan in oat

[0049] (1) Raw material pretreatment

[0050] The raw oatmeal was crushed, passed through a 60-mesh sieve, and ethanol with a final concentration of 75% W / V was added for degreasing and decolorization; centrifuged at 4000r / min for 10min to obtain precipitate 1.

[0051] (2) Amylase method to remove starch

[0052] The precipitate 1 and water were mixed according to the mass volume ratio of 1:24 g:mL, and the pH was adjusted to 6.0. After ultrasonic-assisted extraction at 54°C for 120 min, high-temperature-resistant α-amylase was added to react. Select 3 factors of time, temperature, and enzyme-to-substrate ratio, and set 4 levels according to the orthogonal table L16 (4 5 ) to carry out orthogonal experiments, and according to the results of the experiment, the range and variance analysis were carried out, and the optimal enzymolysis process was obtained as the enzymolysis time was 40min, the temperature was 70°C, and the ...

Embodiment 2

[0098] Example 2 Extraction of β-glucan from oatmeal processing by-products

[0099] (1) Raw material pretreatment

[0100] The raw oat bran was crushed, passed through a 60-mesh sieve, and ethanol with a final concentration of 75% W / V was added to degrease and decolorize; centrifuge at 4000r / min for 10min to obtain precipitate 1.

[0101] (2) Amylase method to remove starch

[0102] The precipitate 1 and water were mixed according to the mass volume ratio of 1:24 g:mL, and the pH was adjusted to 6.0. After ultrasonic-assisted extraction at 54°C for 120 min, high-temperature-resistant α-amylase was added to react. Select 3 factors of time, temperature, and enzyme-to-substrate ratio, and set 4 levels according to the orthogonal table L16 (4 5 ) for orthogonal experiments, and according to the results of the range and variance analysis, the optimal enzymolysis process was obtained as follows: enzymolysis time 10min, temperature 60°C, enzyme-to-substrate ratio 40 U / g, under thi...

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Abstract

The invention relates to the technical field of plant effective component refining and processing, in particular to a method for extracting beta-glucan in oat and oatmeal processing byproducts. The method for extracting the beta-glucan in the oat and oatmeal processing byproducts includes pretreating raw materials; extracting the beta-glucan and removing starch; removing proteins; carrying out concentration and ethanol precipitation; removing heteropolysaccharides; removing ammonium sulfate by means of ethanol precipitation; carrying out freeze drying to obtain extract. The method has the advantages that the oat and oatmeal processing byproducts are smashed, degreased and decolorized, enzymatic starch removal and beta-glucan extraction are simultaneously carried out, accordingly, the problem of difficulty in extracting oat beta-glucan coated in starch granules can be effectively solved, technologies can be simplified, and the cost can be reduced; isoelectric-point protein removal and enzymatic protein removal are combined with each other, and accordingly the protein contents of products can be reduced and reach 4.76%; the final ethanol concentration of ethanol precipitation systemsis 50%, polysaccharides with the molecular weights lighter than 5000 Da can be flocculated under the effective control of the ethanol concentration, and accordingly the purity of the oat beta-glucanproducts can be improved.

Description

technical field [0001] The invention relates to the technical field of refining and processing plant active ingredients, in particular to a method for extracting β-glucan from oat and oatmeal processing by-products. Background technique [0002] β-glucan is a non-starch polysaccharide present in oat endosperm and aleurone layer cell wall, which is composed of uniform D-glucopyranose units through β-(1,3) and β-(1,4) glycosides It is a kind of high molecular polymer, which is a linear macromolecule connected by bonds. β-glucan has many physiological functions such as improving the human immune system, regulating blood sugar, and maintaining the intestinal microbial environment. As a water-soluble active polysaccharide, oat β-glucan also has good water solubility, emulsification, and viscous Therefore, it can be used as a thickener, food functional factor, stabilizer, etc. in the food industry. In the beauty and skin care industry, because of its anti-aging, anti-radiation a...

Claims

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Application Information

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IPC IPC(8): C08B37/02
CPCC08B37/0003C08B37/0024
Inventor 董乐刘宗强苏素珍赵宇婷袁建军王芳
Owner 金维他(福建)食品有限公司
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