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Preparation method and application of pyracantha fortuneana polysaccharide-iron complex

A technology of Pyracantha polysaccharide and complex, which is applied in the field of biomedicine, can solve the problems of weak biological activity and low iron content of polysaccharide-iron complex, and achieves the effects of optimizing process conditions, strong biological activity and increasing iron content.

Active Publication Date: 2019-01-04
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the iron content of polysaccharide-iron complexes prepared by existing chemical synthesis methods is low, resulting in poor biological activity

Method used

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  • Preparation method and application of pyracantha fortuneana polysaccharide-iron complex
  • Preparation method and application of pyracantha fortuneana polysaccharide-iron complex
  • Preparation method and application of pyracantha fortuneana polysaccharide-iron complex

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preparation example Construction

[0050] In order to solve the problem that the iron content of the polysaccharide-iron complex prepared in the prior art is low, the present invention proposes a preparation method of the pyracantha polysaccharide-iron complex, which uses the pyracantha polysaccharide as the glycoside and iron trichloride The reaction prepared Pyracantha polysaccharide iron complex, through the optimization of process conditions, increased the iron content of Pyracantha polysaccharide iron complex. At the same time, because Pyracantha polysaccharide itself has a variety of biological activities, the combination with metal ions can not only provide the body with The safe and non-toxic trace elements also exert the medicinal value of Pyracantha polysaccharide itself, and have stronger biological activity. In one embodiment of the preparation method of the pyracantha polysaccharide-iron complex provided by the present invention, the preparation method of the pyracantha polysaccharide-iron complex c...

Embodiment

[0079] (1) Extraction of Pyracantha polysaccharide

[0080] ①Take 1 kg of fresh Acanthopanax fruit, put it in a vacuum drying oven with a temperature set at 50°C, and dry it to a constant weight. Collect the extract and solid slag separately for 2 hours; dry the solid slag, then add water and extract by heating at 60°C for 3 hours, and collect the extract; combine the extracts of the two extractions to obtain the extract of Acanthopanax ;

[0081] ②Concentrate the Pyracantha extract in a water bath, then concentrate it under reduced pressure and let it stand overnight, discard the resulting precipitate, then remove the protein in the Pyracantha extract by the Sevage method, and remove it by macroporous resin adsorption. Pigment in the Acanthopanax extract to obtain the treated extract;

[0082] ③Adding 95% ethanol solution to the treated extract to make the volume concentration of ethanol reach 80%, then let stand overnight, separate the precipitate precipitated in the solut...

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Abstract

The invention discloses a preparation method and an application of a pyracantha fortuneana polysaccharide-iron complex. The preparation method of the pyracantha fortuneana polysaccharide-iron complexcomprises the steps as follows: adding trisodium citrate to a water solution of pyracantha fortuneana polysaccharide to form a mixed solution under the stirring action, then, adding a ferric chloridesolution and a sodium hydroxide solution until the pH value of the mixed solution is 7-9 to form a reaction solution; heating the reaction solution to 50-70 DEG C to react for 1-2 h, performing cooling, and separating and purifying a reaction product produced in the reaction solution to obtain the pyracantha fortuneana polysaccharide-iron complex. The pyracantha fortuneana polysaccharide is used as glycoside to react with iron chloride to prepare the pyracantha fortuneana polysaccharide-iron complex, and the iron content of the prepared pyracantha fortuneana polysaccharide-iron complex is increased through optimization of the process condition, so that the pyracantha fortuneana polysaccharide-iron complex has higher bioactivity.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method and application of a pyracantha polysaccharide-iron complex. Background technique [0002] Firethorn (Pyracantha fortuneana (Maxim.) Li) fruit, also known as Chiyangzi, Salvation Army Food, its fruit nature and taste are dry and sour, and its medicinal properties have the effects of invigorating the spleen and eliminating stagnation, promoting body fluid and quenching thirst, clearing away heat and detoxification, promoting blood circulation and stopping bleeding. Masses in the chest, food accumulation, metrorrhagia, postpartum congestion, insect repelling and eyesight improvement. Studies in recent years have shown that Pyracantha fruit has great medicinal and edible development value. It is reported that the total extract of Pyracantha japonicus has the effects of eliminating free radicals, lowering blood fat, enhancing immunity, enhancing physical str...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00
CPCA61P35/00C08B37/00C08B37/0003
Inventor 张西锋姚依兰冯阁舒畅王庆
Owner WUHAN POLYTECHNIC UNIVERSITY
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