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Rubber tree HbWRKY83 gene and application thereof

A rubber tree, gene technology, applied in application, genetic engineering, plant genetic improvement and other directions

Inactive Publication Date: 2018-12-28
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the researches on the function of WRKY transcription factors focus on Arabidopsis, rice and other model plants or some economic herb crops, and there are relatively few reports on the function of WRKY in rubber tree.

Method used

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  • Rubber tree HbWRKY83 gene and application thereof
  • Rubber tree HbWRKY83 gene and application thereof
  • Rubber tree HbWRKY83 gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention.

[0041] 1. Materials

[0042] The rubber tree materials used in this experiment were taken from the seedlings (Reyan 7-33-97) planted in the sixth team of the Chinese Academy of Tropical Agricultural Sciences test site and the budding seedlings (Reyan 7-33-97) of the fifth test site. Nicotiana tabacum L. (Nicotiana tabacum L.) (Nicotiana benthamiana) and wild-type Arabidopsis were provided by the Research Group on Mechanism of Dead Skin Prevention and Control.

[0043] Different tissue materials (roots, bark, xylem, buds, latex, leaves) and leaves of rubber trees at different developmental stages (bronze stage leaves, discolored leaves, light green leaves, stable leaves, and senescent leaves) were collected from The seedling trees planted in the sixth team of the Chinese Academy of Tropical ...

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Abstract

The invention provides a rubber tree HbWRKY83 gene. The nucleotide sequence of the gene is as shown in SEQ ID NO:1. The invention further provides HbWRKY83 gene-coded protein and application of the gene. Accordingly, the HbWRKY83 gene is obtained through cloning in a rubber tree for the first time, the gene-coded protein has the transcription factor function and is located in a cell nucleus, and the transcription factor function can be played in the rubber tree. The gene plays an important role in hormone signal transduction such as latex metabolism and ethylene in rubber tree laticiferous cells. After overexpression, resilience such as salt resistance and drought resistance of transgenic plants can be improved, the leaf senescence process can be delayed, and meanwhile the important regulation function is achieved in growth and development of the rubber tree and organism and microorganism adversity stress response. In the future, the plants are subjected to genetic improvement by meansof the HbWRKY83 gene, and effective gene resources can be provided for stress resistance such as plant high salt resistance and drought resistance and delaying leaf senescence.

Description

technical field [0001] The invention relates to the field of biological technology and relates to a rubber tree HbWRKY83 gene and its application. Background technique [0002] As an irreplaceable industrial raw material and an important strategic resource, natural rubber has the characteristics of strong elasticity, good insulation, strong plasticity, airtightness of water and air insulation, and wear resistance, and is widely used in national defense, military industry, medicine, etc. important areas such as health and transportation. At present, the main source of natural rubber is Hevea brasiliensis, which is mainly grown in tropical regions such as Africa, Central America and Asia. As a non-traditional rubber planting area of ​​rubber trees in my country, the production of natural rubber is seriously affected by wind damage, drought, low temperature, cold and heat and other adversity stresses, which has higher requirements for the stress resistance of rubber tree varie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82C12N15/11C12N15/10
CPCC07K14/415C12N15/8261C12N15/8273C12Q1/686C12Q1/6895C12Q2600/13C12Q2600/158C12Q2531/113
Inventor 康桂娟闫栋陈晓丽曾日中杨礼富
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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