Operators, their vectors and their applications
A promoter and promoter sequence technology, applied in the field of bioengineering, can solve the problems of few genetic tools, few types, limited expression activity of promoters, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment approach
[0055] As one of the embodiments of the present invention: select the gene SEQ ID NO: 6 gene coding sequence ATG upstream 355bp to downstream 62bp sequence region of C. glutamicum BZH 001 to design promoter cloning primers 355ZDA-F and ZDA-R (SEQ ID NO: The 6 gene coding sequence ATG starts at the 501bp base of the SEQ ID NO: 6 sequence, and the SEQ ID NO: 6 gene coding sequence is the 501bp ~ 1355bp of the sequence table SEQ ID NO: 6), the clone promoter sequence and its The 62bp sequence (including ATG) after the downstream gene ATG, and a 15bp RBS sequence (AAAGGAGGACAACTA) and two reverse BsaI restriction sites and a BamH I restriction site were sequentially introduced at the 5' end of the downstream primer ZDA-R , and the BamH I restriction site overlaps with the second Bsa I restriction site cut;
[0056] ZDA-F TTCG AAGCTTC TTTGAAGCGGTGTGTGAC (the underline is the enzyme cutting site Hind III)
[0057] ZDA-R
[0058] CCGG GGATCCGAGACC AAAAAAATTTTTT GGTCTC ACATTAG...
PUM
Property | Measurement | Unit |
---|---|---|
molecular weight | aaaaa | aaaaa |
molecular weight | aaaaa | aaaaa |
molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com