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Preparation method of flexible metallic oxide nanofiber phosphorylated peptide enrichment material

A technology of nanofibers and phosphorylated peptides, which is applied in the direction of artificial filaments made of inorganic materials, can solve the problems of low metal oxide content on the fiber surface, poor enrichment capacity of phosphorylated peptides in composite fibers, and easy fracture of nanofiber membranes. Efficient and effective enrichment and purification, effective enrichment and purification, good continuity

Inactive Publication Date: 2018-12-28
XI'AN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing a flexible metal oxide nanofiber phosphorylated peptide enrichment material, which solves the problem that the nanofiber membrane in the prior art is easy to break, and the content of metal oxide on the fiber surface is low under the coating method, which eventually leads to composite The problem of poor enrichment capacity of fiber phosphorylated peptides

Method used

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  • Preparation method of flexible metallic oxide nanofiber phosphorylated peptide enrichment material
  • Preparation method of flexible metallic oxide nanofiber phosphorylated peptide enrichment material
  • Preparation method of flexible metallic oxide nanofiber phosphorylated peptide enrichment material

Examples

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Effect test

Embodiment 1

[0075] Step 1: Dissolve zirconium n-propoxide in isopropanol, add chelating agent triethanolamine after stirring for 5 minutes, and continue stirring for 10 minutes, wherein the ratio of zirconium n-propoxide to isopropanol is 1g:40mL, zirconium n-propoxide and The molar ratio of the chelating agent triethanolamine is 1:0.04; mix uniformly to make a uniform and stable precursor solution with a dynamic viscosity of 0.05Pa s. Stable polycyclic structure, its structural formula is as follows:

[0076]

[0077] Step 2: The above-mentioned precursor solution is made into a precursor nanofiber through an electrospinning forming process. Electrospinning process parameters: spinning temperature is 20°C, relative humidity is 40%, perfusion speed is 1.5mL / h, receiving distance is 20cm, and spinning voltage is 25kV;

[0078] Step 3: Calcining the above-mentioned precursor fibers in the air, the calcination temperature is gradually increased from room temperature to 1200°C, the heatin...

Embodiment 2

[0088] Step 1: Dissolve isopropyl titanate in propanol, stir for 20 minutes, add chelating agent acetylacetone, and continue stirring for 10 minutes, wherein the ratio of isopropyl titanate to propanol is 1g:35mL, isopropyl titanate The molar ratio with the chelating agent acetylacetone is 1:0.03; mix uniformly to make a uniform and stable precursor solution with a dynamic viscosity of 1.0 Pa s. The molecular chain in the precursor solution has acetylacetone ligands and central metal titanium ions. Three-dimensional stable polycyclic structure, its structural formula is as follows:

[0089]

[0090] Step 2: The above-mentioned precursor solution is made into a precursor nanofiber through an electrospinning forming process. Electrospinning process parameters: spinning temperature is 15°C, relative humidity is 45%, perfusion speed is 1.0mL / h, receiving distance is 15cm, and spinning voltage is 25kV;

[0091] Step 3: Calcining the above-mentioned precursor fibers in air, the ...

Embodiment 3

[0093] Step 1: Dissolve iron acetylacetonate in ethanol, add chelating agent nitrilotriacetic acid after stirring for 60 minutes, and continue stirring for 10 minutes, wherein the ratio of iron acetylacetonate to ethanol is 1g:60mL, iron acetylacetonate and chelating agent nitrilotriacetic acid The molar ratio of nitrilotriacetic acid is 1:0.06; mix uniformly to make a uniform and stable precursor solution with a dynamic viscosity of 2Pa s, and the molecular chain in the precursor solution has the nitrilotriacetic acid ligand and the central metal iron ion. Three-dimensional stable polycyclic structure; its structural formula is as follows:

[0094]

[0095] Step 2: The above-mentioned precursor solution is made into a precursor nanofiber through an electrospinning forming process. Electrospinning process parameters: spinning temperature is 22°C, relative humidity is 45%, perfusion speed is 0.5mL / h, receiving distance is 35cm, and spinning voltage is 20kV;

[0096] Step 3:...

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Abstract

The invention discloses a preparation method of a flexible metallic oxide nanofiber phosphorylated peptide enrichment material. The preparation method of the flexible metallic oxide nanofiber phosphorylated peptide enrichment material includes the steps that 1, metal salt is added into corresponding solvent, stirring is conducted to generate metal ions, then, a chelating agent is added, stirring is conducted again, and a precursor solution is obtained, wherein the ratio of the metal salt to the solvent is 1 g:(10-80) ml, and the molar ratio of the metal salt to the chelating agent is 1:(0.01-0.4); 2, the obtained precursor solution is subjected to electrostatic spinning to obtain a precursor nanofiber membrane; 3, the precursor nanofiber membrane is calcined in air, the calcining temperature gradually increases to 500-1200 DEG C from room temperature, calcining is kept for 30-120 min at the highest calcining temperature, and then the flexible metallic oxide nanofiber membrane is obtained. The preparation method of the flexible metallic oxide nanofiber phosphorylated peptide enrichment material is simple in process, and phosphorylated protein and phosphorylated peptide fragments canbe effectively enriched and purified efficiently and repeatedly.

Description

technical field [0001] The invention belongs to the technical field of new materials, and relates to a preparation method of a flexible metal oxide nanofiber phosphorylated peptide enrichment material. Background technique [0002] With the deepening of life science research, phosphoproteomics has become a research hotspot. As an important means of post-translational protein modification, protein phosphorylation regulates the whole process of human life activities, including signal transduction, cell proliferation and cytoskeleton regulation. The research method of protein phosphorylation is to use biological mass spectrometry to identify phosphorylated peptides on the basis of protein enzymatic hydrolysis. However, the phosphorylated peptides show low abundance in the enzymatic hydrolysis products, and the presence of high-abundance non-phosphorylated peptides will inhibit the detection of mass spectrometry signals, and the content of phosphorylated proteins is low, so mas...

Claims

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Application Information

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IPC IPC(8): C04B35/01C04B35/26C04B35/622C04B35/45C04B35/46C04B35/457C04B35/10C04B35/495C04B35/50D01F9/08
CPCC04B35/26C04B35/62218C04B35/62231C04B35/62236C04B35/6225C04B35/62254D01F9/08
Inventor 毛雪刘呈坤阳智吴红韩伟东孙润军
Owner XI'AN POLYTECHNIC UNIVERSITY
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