Nicandra physalodes stem total alkaloid extraction process

Abstract

The invention discloses a nicandra physalodes stem total alkaloid extraction process. The extraction process includes the step A of standard solution preparation: accurately weighing 5.5mg of standardatropine sulfate with a 1 / 10000 scale, and metering the volume with ethanol into a 50 ml volumetric flask to prepare 0.11mg.mL-1 standard solution. First a single factor test is carried out to determine the optimal extraction conditions, and then an orthogonal test is designed based on the optimal extraction conditions obtained by the single factor test. By calculating the alkaloid yield, the nicandra physalodes stem total alkaloid ultrasonic extraction optimal process conditions are obtained: ultrasonic 50min; ratio of material to liquid 1:25; ethanol concentration 60%; ultrasonic power 400w; temperature 50 DEG C; extraction rate 2.58%. The influence of a factor A, a factor B, a factor C, a factor D and a factor E can be analyzed according to analysis of variance, the sequence is that B>A>D>E>C, that is, time>material ratio>concentration>power>temperature.

Description

technical field [0001] The invention relates to the technical field of extracting total alkaloids from the stems of Daqian, in particular to the extraction process of the total alkaloids in the stems of Daqian. Background technique [0002] Daqiansheng is a plant of the Solanaceae plant Physalis genus, native to Peru, also known as Physalis pseudophysalis, Tianzhu, etc., it grows in fields, wastelands, fences, and around houses. It is used for medicinal purposes in both north and south of my country. Or ornamental cultivation, the whole plant has sedative, expectorant, heat-clearing, detoxifying, cough-relieving effects, and can treat mental illness, rabies, cold, rheumatic pain, scabies and other diseases. , rheumatoid arthritis, sores, carbuncles, swelling and pain, etc., the flowers have the functions of dispelling wind, reducing inflammation, and treating nasal sinuses. The raw material of making jelly (also known as ice powder) also has cosmetic value, but its extraction ...

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Problems solved by technology

[0002] Daqiansheng is a plant of the Solanaceae plant Physalis genus, native to Peru, also known as Physalis pseudophysalis, Tianzhu, etc., it grows in fields, wastelands, fences, and around houses. It is used for medicinal purposes in both north and south of my country. Or ornamental cultivation, the whole plant has sedative, expectorant, heat-clearing, detoxifying, cough-relieving effects, and can treat mental illness, rabies, cold, rheumatic pain, scabies and other diseases. , rheumatoid arthritis, sores, carbuncles, swelling and pain, etc., the flowers have the functions of dispelling wind, reducing inflammation, and treating nasal sinuses. The raw material of making jelly (also known as ice powder) also has cosmetic value, but its extraction process of the total alkaloids of the existing stems of the plant is complicated, and the whole extraction process is easily interfered by other factors, resulting in the loss of the total alkaloids of the stems of the plant. The extraction rate is low, for this reason, we propose the extraction process of the total alkaloids in the stems of Daqian

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