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Construction method of feces transplantation experiment piglet model

A technology of fecal transplantation and construction method, which is applied in animal husbandry and other fields, can solve the problems that it is difficult to reflect the immunity or development of flora colonization, the model effect is poor, and the research results of intestinal flora are affected, so as to avoid the treatment of bactericidal drugs and the results Reliable, good results

Inactive Publication Date: 2018-12-28
CHONGQING ACAD OF ANIMAL SCI
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Problems solved by technology

However, when purification treatment methods such as antibiotics or laxatives are used to obtain experimental animals with SPF level or above and carry out experiments such as fecal bacteria transplantation, the drug residues may cause poor model effects, or affect the results of intestinal flora research, and it is difficult to reflect the bacteria. Colonization or the reality of the body's immunity or development

Method used

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  • Construction method of feces transplantation experiment piglet model
  • Construction method of feces transplantation experiment piglet model
  • Construction method of feces transplantation experiment piglet model

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Embodiment 1

[0035] The construction method of feces transplantation experiment piglet model, concrete steps are:

[0036] A. Perform aseptic cesarean section, hysterectomy or hysterotomy on healthy sows, raise newborn sterile piglets in an isolator to maintain air cleanliness; sterilize the drinking water and feed used by piglets;

[0037] B. Take 10g of fresh feces from donor pigs, add 60g of sterilized water and mix evenly, filter with 4 layers of sterilized gauze to obtain a fecal bacteria solution, and then mix the fecal bacteria solution with sterilized glycerin (analytical pure) according to the volume ratio of 8.5 : 1.5 mixed, frozen at 4°C for 15 minutes, then transferred to -80°C for cryopreservation, and then transplanted into sterile piglets for recipient experiments.

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Abstract

The invention belongs to the technical field of medical preparations of inorganic compounds containing ammonia, in particular to a construction method of a feces transplantation experiment piglet model, and the method is obtained by performing feces bacteria transplantation on a sterile newborn piglet. The method of the invention avoids the treatment of antibiotics or other sterilization drugs, the constructed fecal bacteria transplantation pig model has good effect with no drug residue, and the function of donor flora can be reflected more truly and reliably; the expression level of tight junction protein ZO-1 and Occludin in the empty intestine of the piglet can be improved; the development of muscular layer and gland of the intestinal wall of the piglet can be promoted; and the contentof various volatile short-chain fatty acids such as acetic acid, n-propionic acid, isobutyric acid, n-butyric acid, isovaleric acid, n-valeric acid and the like in intestinal tracts of the piglet is improved.

Description

technical field [0001] The invention belongs to the technical field of medical preparations containing ammonia-containing inorganic compounds, and in particular relates to a method for constructing an experimental piglet model for feces transplantation. Background technique [0002] The gut microbiota is not only huge in number, but also functionally important. The intestinal flora not only participates in the energy metabolism of the human body, but also assumes vital physiological functions in the regulatory mechanism of the immune system (“Host-bacterial mutualism in the human intestine”, Bckhed F. et al., Science, 2005, 307(5717):1915-1920, published December 31, 2005). Therefore, it is not an exaggeration to regard the intestinal flora as an independent and inseparable organ of the human body. Not all the communities of the flora are beneficial to the health of the human body. A healthy intestinal flora has its own unique regulation method and maintains a normal dynam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 孙静杜蕾李晓蕾钟航吴梦游小燕曹浩然林玲
Owner CHONGQING ACAD OF ANIMAL SCI
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