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Polyethylene glycol and polypeptide co-modified small-diameter artificial blood vessel and preparation method thereof

A technology of polyethylene glycol and artificial blood vessels, applied in medical science, prostheses, etc., can solve the problem of poor blood compatibility and selective adhesion of endothelial cells, can not effectively prevent thrombosis and intimal hyperplasia, and cannot achieve artificial Rapid endothelialization of blood vessels and other problems, to achieve good blood compatibility, good biodegradability, and beneficial to angiogenesis and reconstruction

Inactive Publication Date: 2018-12-25
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hemocompatibility and selective adhesion of endothelial cells in small-diameter artificial blood vessels prepared by traditional methods are poor, and the rapid endothelialization of artificial blood vessels cannot be achieved, so that the artificial blood vessels cannot effectively prevent thrombosis and endothelialization after long-term in vivo transplantation. Membrane hyperplasia, so that the long-term patency of artificial blood vessels cannot be ensured

Method used

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  • Polyethylene glycol and polypeptide co-modified small-diameter artificial blood vessel and preparation method thereof
  • Polyethylene glycol and polypeptide co-modified small-diameter artificial blood vessel and preparation method thereof
  • Polyethylene glycol and polypeptide co-modified small-diameter artificial blood vessel and preparation method thereof

Examples

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Embodiment 1

[0033] A method for preparing a small-caliber artificial blood vessel co-modified with polyethylene glycol and a polypeptide, comprising the steps of:

[0034] (1) Take 90 parts of PCL-PIBMD and 10 parts of silk fibroin by mass, dissolve them in hexafluoroisopropanol, stir and dissolve at room temperature to make a spinning solution with a concentration of 150 mg / mL;

[0035] The number average molecular weight of the PCL-PIBMD is 50000;

[0036] The number average molecular weight ratio of PCL and PIBMD in the PCL-PIBMD diblock copolymer is 1:1;

[0037] (2) The spinning solution is placed in a syringe, and the syringe is fixed on a single-channel micro-syringe pump, and the processed solution is injected under the conditions of a voltage of 18kV, a receiving distance of 18cm, and an injection rate of 0.7mL / h. Carry out electrospinning on the receiving mold of the above, obtain the tube that thickness is 100 μm, inner diameter is 5mm, and length is 1.5cm;

[0038] Soak the ...

Embodiment 2

[0047] A method for preparing a small-caliber artificial blood vessel co-modified with polyethylene glycol and a polypeptide, comprising the steps of:

[0048] (1) Take 85 parts of PCL-PIBMD and 15 parts of silk fibroin by mass, dissolve them in a mixed solvent of tetrahydrofuran and N,N-dimethylformamide with a volume ratio of 1:1, stir and dissolve at room temperature to prepare Spinning solution with a concentration of 100 mg / mL;

[0049] The number average molecular weight of the PCL-PIBMD is 40000;

[0050] The number average molecular weight ratio of PCL and PIBMD in the PCL-PIBMD diblock copolymer is 1:3;

[0051] (2) The spinning solution is placed in a syringe, and the syringe is fixed on a single-channel micro-syringe pump, and the treated solution is injected under the conditions of a voltage of 15kV, a receiving distance of 15cm, and an injection rate of 0.5mL / h. Carry out electrospinning on the receiving mould, obtain that thickness is 50 μ m, and internal diamete...

Embodiment 3

[0060] A method for preparing a small-caliber artificial blood vessel co-modified with polyethylene glycol and a polypeptide, comprising the steps of:

[0061] (1) Take 95 parts of PCL-PIBMD and 5 parts of silk fibroin by mass, dissolve in a mixed solvent of tetrahydrofuran and N,N-dimethylformamide with a volume ratio of 1:4, stir and dissolve at room temperature to prepare Spinning solution with a concentration of 200mg / mL;

[0062] The number average molecular weight of the PCL-PIBMD is 60000;

[0063] The number average molecular weight ratio of PCL and PIBMD in the PCL-PIBMD diblock copolymer is 1:5;

[0064] (2) The spinning solution is placed in a syringe, and the syringe is fixed on a single-channel micro-syringe pump, and the treated solution is injected under the conditions of a voltage of 20kV, a receiving distance of 20cm, and an injection rate of 0.8mL / h. Carry out electrospinning on the receiving mould, obtain thickness and be 200 μ m, inner diameter is 2mm, th...

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Abstract

The invention discloses a polyethylene glycol and polypeptide co-modified small-diameter artificial blood vessel and a preparation method thereof. The preparation method includes the steps of firstly,dissolving PCL-PIBMD and silk fibroin into hexafluoroisopropanol to obtain a spinning solution; secondly, subjecting the spinning solution to electrospinning to obtain a vessel 50-200 micrometers inthickness, 1-5mm in inner diameter and 2cm in length; thirdly, soaking the vessel into a first solution to obtain a surface aminated vessel, wherein the first solution is prepared by adding dopamine and hexanediamine into a Tris-HCl buffer solution; fourthly, adding bifunctional polyethylene glycol and cell adhesion peptides into a second solution to obtain a third solution, and soaking the surface aminated vessel into the third solution to obtain the polyethylene glycol and polypeptide co-modified small-diameter artificial blood vessel. The preparation method has the advantages that the method is simple and practicable to operate; the prepared small-diameter artificial blood vessel is good in biocompatibility and biodegradability, capable of inhibiting the adhesion of blood components tothe surface of the artificial blood vessel, capable of preventing thrombosis and intimal hyperplasia and less prone to restenosis and restenosis.

Description

technical field [0001] The invention relates to a small-diameter artificial blood vessel co-modified with polyethylene glycol and polypeptide, its preparation method and application, and belongs to the technical field of medical polymer materials. technical background [0002] With the improvement of people's living standards, the incidence of cardiovascular diseases has increased year by year, causing high mortality and huge social burden worldwide. In clinical treatment, bypass surgery is usually performed on patients, and autologous blood vessels, especially autologous saphenous vein and internal mammary artery, have always been regarded as materials for the treatment of vascular occlusion. However, autologous vascular grafts also have several limitations, such as size or length mismatch, prior surgery, and donor site morbidity. Artificial blood vessels are also used to replace autologous blood vessels. The artificial vascular grafts commonly used clinically include poly...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/50A61L27/58
CPCA61L27/26A61L27/507A61L27/58A61L2300/252A61L2300/42C08L67/04C08L89/00C08L71/02
Inventor 冯亚凯白凌闯赵静任相魁郭锦棠
Owner TIANJIN UNIV
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