A biological agent capable of inhibiting the growth of Vibrio in shrimp ponds, its preparation method and application
A biological agent and Vibrio technology, applied in the field of aquaculture water quality control, can solve the problems of not being able to adapt to the nutrient environment of aquaculture wastewater, poor resistance to adverse environmental impacts, and unstable treatment effects, so as to improve the body's immunity, efficiently and safely ammonia nitrogen Harm, improve the effect of water safety
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Embodiment 1
[0047] 1. Preparation of starter
[0048] 1) Preparation of activated medium
[0049] The used activation medium for the activation of the starter comprises the following components by weight: 1000 parts of culture pond water, 20 parts of brown sugar, 0.5 part of ammonium sulfate, 0.05 part of magnesium sulfate heptahydrate, 0.5 part of dipotassium hydrogen phosphate, 14.29 parts of calcium carbonate, 2 parts of sodium chloride, 1 part of brine, 0.02 part of calcium chloride monohydrate, 0.05 part of ferrous sulfate heptahydrate, and 0.05 part of copper sulfate pentahydrate.
[0050] 2) Activation of complex bacteria A:
[0051] Add the activation medium prepared in 1) by means of aerobic activation, and activate it by culturing at 30°C for 36 hours to obtain fermentation broth A.
[0052] 3) Activation of complex bacteria B:
[0053] Add the activation medium prepared in 1) by sealing and activating, culture at 30° C. for 36 hours for activation, and obtain fermentation br...
Embodiment 2
[0061] 1. Preparation of starter
[0062] 1) Preparation of activated medium
[0063] The used activation medium for the activation of the starter comprises the following components by weight: 1000 parts of culture pond water, 20 parts of brown sugar, 0.5 part of ammonium sulfate, 0.05 part of magnesium sulfate heptahydrate, 0.5 part of dipotassium hydrogen phosphate, 14.29 parts of calcium carbonate, 2 parts of sodium chloride, 1 part of brine, 0.02 part of calcium chloride monohydrate, 0.05 part of ferrous sulfate heptahydrate, and 0.05 part of copper sulfate pentahydrate.
[0064] 2) Activation of complex bacteria A:
[0065] Add the activation medium prepared in 1) by means of aerobic activation, and activate it by culturing at 27°C for 48 hours to obtain fermentation broth A.
[0066] 3) Activation of complex bacteria B: add the activation medium prepared in 1) by sealing and activating, culture at 27° C. for 48 hours for activation, and obtain fermentation broth B.
[0...
Embodiment 3
[0074] 1. Preparation of starter
[0075] 1) Preparation of activated medium
[0076] The used activation medium for the activation of the starter comprises the following components by weight: 1000 parts of culture pond water, 20 parts of brown sugar, 0.5 part of ammonium sulfate, 0.05 part of magnesium sulfate heptahydrate, 0.5 part of dipotassium hydrogen phosphate, 14.29 parts of calcium carbonate, 2 parts of sodium chloride, 1 part of brine, 0.02 part of calcium chloride monohydrate, 0.05 part of ferrous sulfate heptahydrate, and 0.05 part of copper sulfate pentahydrate.
[0077] 2) Activation of complex bacteria A:
[0078] Add the activation medium prepared in 1) by means of aerobic activation, culture at 30° C. for 24 hours for activation, and obtain fermentation broth A.
[0079] 3) Activation of complex bacteria B:
[0080] Add the activation medium prepared in 1) by sealing and activating, culture at 30° C. for 24 hours for activation, and obtain fermentation brot...
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