Seed culture medium for acremonium terricola, and application of seed culture medium
A technology of Acremonium terrestris and a culture medium, which is applied to the field of Acremonium terrestris seed culture medium, can solve the problems of limited application and development, expensive price of Cordyceps gounia, etc., and achieves the effects of short cultivation time and high content of active ingredients.
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Embodiment 1
[0022] Embodiment 1: the preparation method of Acremonium acremonium seed culture medium
[0023] Prepare the seed culture medium according to the content of each component in Table 1. After the preparation is completed, autoclave, cool, and maintain the pressure with sterile air. The sterilized seed medium was free of bacteria culture, and the pH was adjusted to 6 after sterilization.
[0024] The protective agent described in Table 1 is composed of polyvinylpyrrolidone, anhydrous fructose and yeast β-glucan, and the weight ratio of each component is 2:1:5.
[0025] Table 1 Seed medium composition
[0026]
Embodiment 2
[0027] Embodiment 2: the preparation of Acremonium acremonium culture
[0028] 1. Seed cultivation
[0029] The screened good Acremonium acremonium was divided into four groups, inoculated into the slant medium, and cultivated at 25°C for 4 days. The components of the slant medium were: potato 150g / L, glucose 15g / L, water 1000ml and agar powder 20g / L, pH 6, the inclined test tube bacterial strain was obtained; the obtained inclined test tube bacterial strain was cultured in a shake flask, and then moved to a seed tank for seed cultivation, the cultivation temperature was 30° C., the rotation speed was 250 rpm, and the cultivation was carried out for 20 hours to obtain the seed cultivation liquid.
[0030] 2. Fermentation
[0031] Inoculate the prepared seed culture solution into a fermenter equipped with a fermentation medium according to the inoculation amount of 20-25%, and ferment. The components of the fermentation medium are: sucrose 5g / L, glucose 20g / L, peptone 10g / L, ...
Embodiment 3
[0042] Embodiment 3: Effect test on growth performance and immune function of piglets
[0043] 40 25-day-old just-weaned piglets with similar body weight were selected and randomly divided into 4 groups before the start of the formal experiment, with 10 pigs in each group, and each group was reared in pens. The 4 groups are respectively set as:
[0044] Group 1: Cordycepin group (addition amount of cordycepin: 300mg / kg feed)
[0045] The 2nd group: drug control group (addition amount of 10% colistin sulfate: 200mg / kg feed)
[0046] The third group: drug control group (50% astragalus polysaccharide addition: 500mg / kg feed)
[0047] Group 4: blank control group (feed for weaned piglets, without adding any drugs)
[0048] Each group was continuously fed for 20 days from the age of 25 days, free to eat, from the age of 25 days to the end of the age of 45 days, all experimental pigs were inoculated with classical swine fever vaccine once on the first day of administration, and e...
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