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Seed culture medium for acremonium terricola, and application of seed culture medium

A technology of Acremonium terrestris and a culture medium, which is applied to the field of Acremonium terrestris seed culture medium, can solve the problems of limited application and development, expensive price of Cordyceps gounia, etc., and achieves the effects of short cultivation time and high content of active ingredients.

Inactive Publication Date: 2018-12-21
GUANGDONG RONGDA BIOENG CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many important secondary metabolites in Cordyceps guni, such as cordycepic acid, cordycepin, polysaccharides, etc., but the expensive price of Cordyceps guni limits its application and development

Method used

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  • Seed culture medium for acremonium terricola, and application of seed culture medium
  • Seed culture medium for acremonium terricola, and application of seed culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the preparation method of Acremonium acremonium seed culture medium

[0023] Prepare the seed culture medium according to the content of each component in Table 1. After the preparation is completed, autoclave, cool, and maintain the pressure with sterile air. The sterilized seed medium was free of bacteria culture, and the pH was adjusted to 6 after sterilization.

[0024] The protective agent described in Table 1 is composed of polyvinylpyrrolidone, anhydrous fructose and yeast β-glucan, and the weight ratio of each component is 2:1:5.

[0025] Table 1 Seed medium composition

[0026]

Embodiment 2

[0027] Embodiment 2: the preparation of Acremonium acremonium culture

[0028] 1. Seed cultivation

[0029] The screened good Acremonium acremonium was divided into four groups, inoculated into the slant medium, and cultivated at 25°C for 4 days. The components of the slant medium were: potato 150g / L, glucose 15g / L, water 1000ml and agar powder 20g / L, pH 6, the inclined test tube bacterial strain was obtained; the obtained inclined test tube bacterial strain was cultured in a shake flask, and then moved to a seed tank for seed cultivation, the cultivation temperature was 30° C., the rotation speed was 250 rpm, and the cultivation was carried out for 20 hours to obtain the seed cultivation liquid.

[0030] 2. Fermentation

[0031] Inoculate the prepared seed culture solution into a fermenter equipped with a fermentation medium according to the inoculation amount of 20-25%, and ferment. The components of the fermentation medium are: sucrose 5g / L, glucose 20g / L, peptone 10g / L, ...

Embodiment 3

[0042] Embodiment 3: Effect test on growth performance and immune function of piglets

[0043] 40 25-day-old just-weaned piglets with similar body weight were selected and randomly divided into 4 groups before the start of the formal experiment, with 10 pigs in each group, and each group was reared in pens. The 4 groups are respectively set as:

[0044] Group 1: Cordycepin group (addition amount of cordycepin: 300mg / kg feed)

[0045] The 2nd group: drug control group (addition amount of 10% colistin sulfate: 200mg / kg feed)

[0046] The third group: drug control group (50% astragalus polysaccharide addition: 500mg / kg feed)

[0047] Group 4: blank control group (feed for weaned piglets, without adding any drugs)

[0048] Each group was continuously fed for 20 days from the age of 25 days, free to eat, from the age of 25 days to the end of the age of 45 days, all experimental pigs were inoculated with classical swine fever vaccine once on the first day of administration, and e...

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Abstract

The invention relates to a seed culture medium for acremonium terricola, and application of the seed culture medium. The seed culture medium is prepared from the following components: sucrose, peptone, yeast powder, corn starch, magnesium sulfate, potassium dihydrogen phosphate and a protective agent, and the pH value of the seed culture medium is 5-7. The seed culture medium provided by the invention can promote the rapid and mass propagation of the acremonium terricola, and can remarkably increases the content of cordycepin in the acremonium terricola.

Description

technical field [0001] The invention relates to the field of fungal culture and fermentation, in particular to a acremonium acremonium seed culture medium and application thereof. Background technique [0002] Guni Cordyceps is a rare Chinese Cordyceps medicinal material, which has the effects of improving sleep, regulating immunity, calming and relieving pain. There are many important secondary metabolites in Cordyceps guni, such as cordycepic acid, cordycepin, polysaccharides, etc., but the high price of Cordyceps guni limits its application and development. Therefore, the artificial culture that replaces Guni Cordyceps arises at the historic moment. Acremonium terricola culture is a product obtained by artificial fermentation of Acremonium terricola isolated from Cordyceps guni. It has functional ingredients similar to Cordyceps guni, such as cordycepin, cordycepic acid, and polysaccharides. The cost of the Acremonium acremonium culture obtained by fermentation is great...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P1/02C12R1/645
CPCC12N1/14C12P1/02C12N1/145C12R2001/645
Inventor 张文唐谢芳张泳郑雪媚林万华黄炜乾方文棋吴阳开刘宗新蒋顺进孟葵开
Owner GUANGDONG RONGDA BIOENG CO LTD
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