Celery hybrid combination method

Abstract

The invention discloses a celery hybrid combination method. According to the method, firstly through transformation, a plurality of celery cytoplasmic male sterile (CMS) lines with stable heredity areobtained; the CMS lines are used as female parents; an excellent selfing line is selected and determined to be used as male parents; the fine management is performed in winter; the plants are cultured into healthy and strong breeding plants; the low-temperature vernalization process is completed; in spring, according to the hybrid combination plan, several CMS female parents and corresponding male parents are subjected to simultaneous fixed planting into one seed collecting ridged field; in a period from bolting to blooming, a high-density isolation net chamber is erected above the seed collecting ridged field, so that all female and male parents in the net chamber are isolated from external pollen; then, a low-density isolation net bag is used in the net chamber for performing flower andbranch isolation on all CMS female parent plants. The artificial auxiliary pollination is performed in the blooming period; the hybrid seeds can be harvested from the CMS female parent plants when seeds become yellow. The condition that one male parent simultaneously perform hybrid combination with a plurality of CMS female parents can be realized by the method; the sufficient quantity of hybridseeds are obtained; the hybrid breeding efficiency and the plant disease and insect pest prevention and control effects are improved; the field management is convenient.

Description

technical field [0001] The invention belongs to the technical field of vegetable planting, and relates to a method for hybridization and assembly of celery. Background technique [0002] Celery is a biennial vegetable of the Umbelliferae genus Celery. It is an insect-borne flower and is often cross-pollinated. The flowers are small and large, and the flowering branches are soft and overlapping. The flowering period is long and scattered. This special flowering habit makes it difficult for celery to undergo strict artificial emasculation and cross-pollination. Make the celery hybrid breeding work can not realize the breakthrough progress for a long time. The application of the male sterile line of celery has solved this difficult problem well, and opened up an effective way for the work of cross breeding of celery. Chinese invention patent ZL201010300772.9 "Method for Propagation and Hybrid Seed Production of Celery Recessive Nuclear Gene Male Sterile Line" reproduces celery...

AI Technical Summary

Problems solved by technology

Chinese invention patent ZL201010300772.9 "Method for Propagation and Hybrid Seed Production of Celery Recessive Nuclear Gene Male Sterile Line" reproduces celery recessive nuclear gene male sterile line through asexual reproduction and realizes the goal of celery hybrid seed production, but the The method and technology are relatively complicated, and large-scale hybrid seed production has certain limitations

Chinese invention patent ZL201410705174.8 "A method and application of cross-pollination of celery" provides a cross-pollination method for cytoplasmic male sterility (CMS) of celery, so that the CMS female parent plants in the celery hybrid combination can obtain parent While this plant is effectively pollinated, 100% pollen isolation is achieved between different combinations, but the breeding efficiency of the one-to-one hybrid pollination and isolation of celery parent plants determined by this method is relatively low, and the pollination effect is poor. Problems such as less, timely and incomplete control of diseases and insect pests, and inconvenient field management need to be solved through technological innovation