Method for extracting Kadsura coccinea fruit polysaccharides
An extraction method and fruit technology, which is applied in the field of polysaccharide extraction of black tiger fruit, can solve the problem that Feng Yi et al. found that the root of black tiger has antibacterial effect on pneumococcus, and the antibacterial effect of pericarp on Salmonella typhi. Research by GohMJ et al. It shows that the black tiger extract can inhibit the synthesis of melanin. Wang Laiyou and others found that the black tiger extract can regulate alcoholic fatty liver and protect the liver. Peng Mijun and others found that the pulp is rich in amino acids and trace elements. Sun Jian et al. , few reports and other issues, the extraction method is simple and feasible, the dosage is reduced, and the effect of antioxidant activity is good
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Embodiment 1
[0022] A method for extracting black tiger fruit polysaccharides, comprising the following steps:
[0023] Step 1, taking the black tiger fruit and then washing, removing seeds, drying, crushing, and sieving to obtain fruit powder;
[0024] Step 2, take the fruit powder and the ethanol aqueous solution with a mass fraction of 92% according to the mass ratio of 1:2.8, reflux and stir for 1 h, filter to obtain a filter residue, and repeat the above steps three times;
[0025] Step 3: Take 1 part by mass of filter residue and 20 parts by mass of distilled water to form a mixed aqueous solution, reflux and stir for 3.5 hours, concentrate the mixed aqueous solution to 5 parts by mass, add 18 parts by mass of absolute ethanol, store in cold storage at 0°C for 12 hours, centrifuge, and remove the lower layer of sediment Wash with absolute ethanol, acetone and ether in sequence, and dry to obtain crude polysaccharide;
[0026] Step 4, taking the crude polysaccharide and performing de...
Embodiment 2
[0028] A method for extracting black tiger fruit polysaccharides, comprising the following steps:
[0029] Step 1, taking the black tiger fruit and then washing, removing seeds, drying, crushing, and sieving to obtain fruit powder;
[0030] Step 2, take the fruit powder and the ethanol aqueous solution with a mass fraction of 98% according to the mass ratio of 1:3.2, reflux and stir for 2 hours, filter to obtain a filter residue, and repeat the above steps three times;
[0031] Step 3: Take 1 part by mass of filter residue and 30 parts by mass of distilled water to form a mixed aqueous solution, reflux and stir for 4.5 hours, concentrate the mixed aqueous solution to 6 parts by mass, add 22 parts by mass of absolute ethanol, store in a refrigerator at 4°C for 16 hours, centrifuge, and remove the lower layer of sediment Wash with absolute ethanol, acetone and ether in sequence, and dry to obtain crude polysaccharide;
[0032] Step 4, taking the crude polysaccharide and perform...
Embodiment 3
[0034] A method for extracting black tiger fruit polysaccharides, comprising the following steps:
[0035] Step 1, taking the black tiger fruit and then washing, removing seeds, drying, crushing, and sieving to obtain fruit powder;
[0036] Step 2, take the fruit powder and the ethanol aqueous solution with a mass fraction of 95% according to the mass ratio of 1:3, reflux and stir for 1.5h, filter to obtain a filter residue, and repeat the above steps three times;
[0037] Step 3: Take 1 part by mass of filter residue and 25 parts by mass of distilled water to form a mixed aqueous solution, reflux and stir for 4 hours, concentrate the mixed aqueous solution to 5.5 parts by mass, add 20 parts by mass of absolute ethanol, store in cold storage at 2°C for 14 hours, centrifuge, and remove the lower layer of precipitation in turn Wash with absolute ethanol, acetone, ether, and dry to obtain crude polysaccharide;
[0038] Step 4, taking the crude polysaccharide and performing depro...
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