Method for extracting active substance from guava leaves

Abstract

The invention discloses a method for extracting an active substance from guava leaves. The method comprises the following steps: (1) pulverizing the guava leaves and sieving the material, and then using an ethanol solution containing ionic liquid as a solvent for ultrahigh pressure extraction; 2) performing ultrasonic extraction on an obtained extract, performing centrifugation on the obtained extract, and taking a supernatant for standby; and (3) stirring the supernatant while adding ethanol, performing standing and centrifugal filtering and performing freeze-drying on the precipitate to obtain the active substance. The extraction method of the invention combines the ultrasonic wave and a high pressure technology without interference, and realizes the coordinated treatment of the ultrasonic wave and a high pressure reaction, the mechanical effect, warming effect, physical and chemical effect (including dispersion effect, thixotropic effect, and cavitation effect) of ultrasonic waves,as well as excellent directionality, strong penetrating ability, and high efficiency and low energy consumption of ultra-high pressure extraction are used, the extraction rate can be effectively increased, energy consumption can be reduced, the extraction time can be saved, and the method is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of extracting active substances from guava leaves, in particular to a method for extracting active substances from guava leaves. Background technique [0002] The comprehensive utilization of plant resources is the focus of people's development and research in recent years, and the rational development of effective active and functional substances in plants is of great significance. Guava (Psidium guajava L) is a plant of the genus Guava in the Myrtaceae family. It is a small tropical evergreen tree or shrub. In tropical America, it spread to every corner of the tropical and subtropical regions of the world with Spanish and Portuguese colonists in the 17th century. In recent years, my country's Guangxi, Fujian, Hainan, Guangdong and other places have introduced and planted from Taiwan. Native Americans and the Blang people in Yunnan have the custom of using fresh leaves to treat enteritis and dysentery. Traditional...

AI Technical Summary

Problems solved by technology

In recent years, the rapid development of guava leaf active substance extraction technology includes supercritical fluid extraction technology, ultrasonic extraction, microwave extraction technology, two-phase extraction, enzyme engineering technology and ultra-high pressure extraction technology, etc., in terms of extraction effect and extraction conditions, etc. All aspects are superior to traditional methods, but there are still problems such as high extraction energy consumption, long extraction time and complicated process