A kind of hyaluronic acid, its preparation method and application
A technology of hyaluronic acid and other aspects, applied in the field of cosmetics, can solve the problems that hyaluronic acid is difficult to be absorbed by the human body, and the moisturizing effect cannot be lasting.
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Embodiment 1
[0041] Example 1 Preliminary Screening
[0042] In order to develop hyaluronic acid products with better performance, the inventors first prepared HA with different PD and molecular weight ranges.
[0043] Preparation of HA
[0044] The hyaluronic acid of the present invention is obtained by fermenting and purifying Streptococcus zooepidemicus (Sterptococcus zooepidemicus, purchased from ATCC, catalog number HA-116ATCC39920), and the specific method is as follows:
[0045] culture medium
[0046] Slant medium (g / L): Brain Heart Infusion (BHI) 36, glucose 12, yeast powder 12, agar powder 24, pH 7.2.
[0047] Seed medium (g / L): glucose 40, yeast powder 20, magnesium sulfate heptahydrate 2.0, manganese sulfate tetrahydrate 0.1, potassium dihydrogen phosphate 2.0, calcium carbonate 20, pH 7.0.
[0048] Fermentation medium (g / L): yeast powder 20, disodium hydrogen phosphate 6.2, potassium sulfate 1.3, glucose 140, magnesium sulfate heptahydrate 2.0, pH 7.0.
[0049] trainin...
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