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Preparation method and application of biosynthesized composite organic trace elements

A trace element and biosynthesis technology, which is applied in the field of biotechnology and feed production, can solve the problems of low absorption and utilization rate of trace elements, reduced efficacy, and more waste, so as to improve the utilization rate of feed, improve biological titer, Reduce the effect of antagonism

Pending Publication Date: 2018-12-14
湖北华大瑞尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, trace elements in livestock and poultry feed have been added in the form of inorganic minerals. Experiments have shown that: (1) The absorption and utilization rate of trace elements is low, generally not more than 20%, by adding inorganic minerals to supplement trace elements. As a result, a large amount of trace elements are discharged, causing heavy metal pollution in the environment; (2) poor palatability, because metal minerals have a bitter taste or a unique smell, which affects the feed intake of livestock and poultry; (3) instability, inorganic minerals enter the digestive system , forming the free state of metal ions, which are easy to combine with oxalic acid and phytic acid to form insoluble matter with stable structure, thus causing the loss of vitality of trace elements; activity, reducing its efficacy
[0003] Organic trace elements are metal ions and organic compounds such as proteins, small peptides, amino acids, organic acids, polysaccharides, etc. through covalent bonds or ionic bonds to form stable chelates or complexes. Practice has proved that organic trace elements can improve animal health. The production performance of poultry and the improvement of meat quality, etc., but at present, the synthesis of organic trace elements is mainly obtained by physical and chemical methods, which requires a multi-step process, and needs to control different pH in each link, the operation is complicated, and the waste generated more, causing pollution to the environment. At the same time, the organic trace elements obtained by physical and chemical methods are generally single or two types of organic trace elements, and it is impossible to obtain a compound of multiple organic trace elements at the same time. Therefore, the feeding value is single and the cost is low. high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A method for biosynthesizing compound organic trace elements, comprising the following steps:

[0056] Step (1): respectively inoculate Bacillus subtilis, Bacillus natto, Rhodotorula viscosus, Enterococcus faecium and Pediococcus lactis in a sterilized separate medium for culture, and the components of the medium are: molasses 2wt%, yeast extract 1wt%, magnesium sulfate 0.2wt%, potassium dihydrogen phosphate 0.2wt%, water 96.6wt%;

[0057] The culture temperature of the Bacillus subtilis, Bacillus natto, Enterococcus faecium and lactic acid flakes are all 37°C, and the culture temperature of Rhodotorula viscosus is 28°C;

[0058] The cultivation time of the Bacillus subtilis, Bacillus natto, Enterococcus faecium, Pediococcus lactis and Rhodotorula viscosus were all 14 hours;

[0059] The shaking table speed when described Bacillus subtilis, Bacillus natto and Rhodotorula viscosum are cultivated is 250r / min;

[0060] The shaking table speed when described Enterococcus ...

Embodiment 2

[0074] A method for biosynthesizing compound organic trace elements, comprising the following steps:

[0075]Step (1): respectively inoculate Bacillus subtilis, Bacillus natto, Rhodotorula viscosus, Enterococcus faecium and Pediococcus lactis in a sterilized separate medium for culture, and the components of the medium are: molasses 2wt%, yeast extract 1wt%, magnesium sulfate 0.2wt%, potassium dihydrogen phosphate 0.2wt%, water 96.6wt%;

[0076] The culture temperature of the Bacillus subtilis, Bacillus natto, Enterococcus faecium and lactic acid flakes are all 37°C, and the culture temperature of Rhodotorula viscosus is 28°C;

[0077] The culture time of described Bacillus subtilis, Bacillus natto, Enterococcus faecium, Pediococcus lactis and Rhodotorula viscosus were all 16 hours;

[0078] The shaking table speed when described Bacillus subtilis, Bacillus natto and Rhodotorula viscosum are cultivated is 200r / min;

[0079] The shaking table speed when described Enterococcus...

Embodiment 3

[0093] A method for biosynthesizing compound organic trace elements, comprising the following steps:

[0094] Step (1): respectively inoculate Bacillus subtilis, Bacillus natto, Rhodotorula viscosus, Enterococcus faecium and Pediococcus lactis in a sterilized separate medium for culture, and the components of the medium are: molasses 2wt%, yeast extract 1wt%, magnesium sulfate 0.2wt%, potassium dihydrogen phosphate 0.2wt%, water 96.6wt%;

[0095] The culture temperature of the Bacillus subtilis, Bacillus natto, Enterococcus faecium and lactic acid flakes are all 37°C, and the culture temperature of Rhodotorula viscosus is 28°C;

[0096] The culture time of described Bacillus subtilis, Bacillus natto, Enterococcus faecium, Pediococcus lactis and Rhodotorula viscosus were all 15 hours;

[0097] The rotating speed of the shaking table during the cultivation of described Bacillus subtilis, Bacillus natto and Rhodotorula viscosus is 230r / min;

[0098] The shaking table speed when...

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Abstract

The invention discloses a preparation method and an application of biosynthesized composite organic trace elements. The method comprises the following steps: (1) separately preparing every single bacterial liquid; (2) preparing a mixed bacterial liquid from all the bacterial liquids prepared in step (1); (3) adding a composite inorganic trace element material to a carrier material to prepare a fermentation substrate; (4) mixing the mixed bacterial liquid with the fermentation substrate to obtain a material to be fermented; and (5) performing sealing fermentation on the material to be fermentedto obtain the composite organic trace element material. An application method of the prepared composite organic trace element material is characterized in that 2.5-5 wt% of the composite organic trace element material is added into a conventional diet feed free from trace elements in order to prepare a compound feed. The composite organic trace elements prepared in the invention can improve the absorption and utilization ratio of trace elements, reduce the addition amount of trace elements in the feed, reduce the pollution of the emission of trace elements to soil, improve the production performance of animas and reduce the breeding cost.

Description

technical field [0001] The invention belongs to the field of biotechnology and feed production, and specifically relates to a method for biosynthesizing and compounding organic trace elements and its application. Background technique [0002] Trace elements are nutrients that must be added to livestock and poultry animal feed. They can directly or indirectly participate in almost all physiological and biochemical activities of livestock and poultry animals. important role. For a long time, trace elements in livestock and poultry feed have been added in the form of inorganic minerals. Experiments have shown that: (1) The absorption and utilization rate of trace elements is low, generally not more than 20%, by adding inorganic minerals to supplement trace elements. As a result, a large amount of trace elements are discharged, causing heavy metal pollution in the environment; (2) poor palatability, because metal minerals have a bitter taste or a unique smell, which affects the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K20/20A23L3/3508
CPCA23K10/12A23K10/37A23K20/30A23L3/3508A23V2002/00A23V2400/413A23V2200/10Y02P60/87
Inventor 李筱雯李钢平姜涛韩继宏崔卫涛况世昌欧阳潮龙兴权
Owner 湖北华大瑞尔科技有限公司
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