A microfluidic chip and detection method for cell culture and simulated post-exercise pulsating shear stress environment

A microfluidic chip and cell culture technology, applied in the field of cell biomechanical experimental devices, can solve the problems of different biological responses and achieve the effect of complete distribution

Active Publication Date: 2022-01-18
DALIAN UNIV OF TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the post-exercise shear stress waveform simulated by microfluidic chip technology is mainly steady flow shear stress, and a large number of in vitro experiments have confirmed that the biological responses of vascular endothelial cells to steady flow and pulsatile flow are significantly different.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A microfluidic chip and detection method for cell culture and simulated post-exercise pulsating shear stress environment
  • A microfluidic chip and detection method for cell culture and simulated post-exercise pulsating shear stress environment
  • A microfluidic chip and detection method for cell culture and simulated post-exercise pulsating shear stress environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0054] The technical solutions of the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0055] A microfluidic chip and detection method for cell culture and simulated post-exercise pulsating shear stress environment, such as figure 1 As shown, the microfluidic chip includes a cell culture system A, a shear force waveform generation system B and a shear force waveform detection system C;

[0056] Cell culture system A is mainly composed of cell culture chamber 1-3, cell suspension inlet 1-1, cell suspension outlet 1-2 and microchannels; Microchannels, m≥3, the microchannels on the upper side and the microchannels on the lower side are arranged symmetrically; the outlets of the upper and lower microchannels are evenly distributed on the long axis of the upper and lower sides of the cell culture chamber 1-3; the outlets of the upper microchannels Collect and connect with the cell suspension inlet 1-1, and the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
widthaaaaaaaaaa
heightaaaaaaaaaa
Login to view more

Abstract

A microfluidic chip and detection method for cell culture and simulated post-exercise pulsating shear stress environment, belonging to the technical field of cell biomechanical experimental devices. The microfluidic chip includes three parts: a cell culture system, a shear force waveform generation system and a shear force waveform detection system. The cell culture system is composed of cell culture chamber, cell suspension inlet and outlet and microchannel; the shear force waveform generation system is composed of cell culture chamber, cell culture fluid inlet and outlet, elastic chamber, resistance channel and microchannel; by adjusting the cell culture The flow input waveform of the liquid inlet and the size of the rear resistance channel are changed to realize the loading of the shear force waveform at rest and after exercise in the cell culture chamber; the shear force waveform detection system consists of pressure detection microchannels on both sides of the cell culture chamber The configuration is to record the change of the liquid column in the pressure detection channel through a microscope, and then calculate the shear force waveform in the cell culture chamber through the pressure values ​​on both sides of the cell culture chamber.

Description

technical field [0001] The invention belongs to the technical field of cell biomechanics experiment equipment, and is based on hemodynamic principles and microfluidic chip technology, and is used to study the influence of exercise-induced shear force signals on the morphology and function of vascular endothelial cells and its molecular biological mechanism The experimental device is specifically a microfluidic chip and a detection method for cell culture and simulated pulsating shear force environment after exercise. Background technique [0002] Vascular endothelial cells are located in the innermost layer of the blood vessel wall, the inner side is in direct contact with the flowing blood, and the outer side is adjacent to smooth muscle cells. Vascular endothelial cells are not only a selective physical barrier between blood and vascular tissue, they can also pass through receptors and receptors on the cell membrane surface, such as polysaccharide-protein complexes, tyrosi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/34
CPCC12M23/16C12M35/04C12M41/40
Inventor 王艳霞覃开蓉刘书田薛春东李泳江于洪建杨雨浓张文佳
Owner DALIAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap