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Sterile line mutant acquisition method

A method of obtaining mutants and a technology for obtaining mutants, which are applied in the field of obtaining mutants of sterile lines, can solve the problems of prolonging the creation cycle of the third-generation intelligent CMS and affecting the breeding process of the third-generation hybrid rice, and achieve easy operation and avoid Uncertainty, the effect of improving acquisition efficiency

Inactive Publication Date: 2018-12-11
QINGDAO YUANCE GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Existing technologies cannot overcome various problems in the process of creating fertility mutants, and these defects will prolong the creation cycle of the third-generation intelligent sterile line, thus affecting the process of third-generation hybrid rice breeding

Method used

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  • Sterile line mutant acquisition method
  • Sterile line mutant acquisition method
  • Sterile line mutant acquisition method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Obtaining of rice flower fragrance sterile mutant

[0022] We used the EAT1 mutant male sterile line Wuyunjing No. 7 as the donor parent and Daohuaxiang as the recipient parent. Through one crossing, two backcrossings, supplemented by phenotypic screening, we only spent a small amount of The male sterile mutant of Daohuaxiang was obtained at human cost. Field identification shows that the male sterility rate is as high as 99.5%.

Embodiment 2

[0023] Example 2: Obtaining Nipponbare Sterile Mutants

[0024] We used the EAT1 mutant male sterile line Wuyunjing No. 7 as the donor parent, and Nipponbare as the recipient parent. Through one crossing, two backcrossings, and phenotypic screening, we only spent a small amount of Nippon Haru male sterile mutants were obtained at human cost. Field identification shows that the male sterility rate is as high as 99.5%.

[0025] DNA extraction

[0026] 1. Take 0.5g of rice leaf from each BC1 plant and put it in a research body frozen at -20°C. Add liquid nitrogen and grind it into a white powder, and put it into a 2mL centrifuge tube. While grinding, turn on the switch of the water bath to heat to 65°C.

[0027] 2. Add 700 μL of CTAB lysate preheated at 65°C for 1 hour to the centrifuge tube, turn over and shake several times, and then put it in a constant temperature water bath at 65°C for 40 minutes (every 20 minutes, turn over and shake several times)

[0028] 3. Cool the...

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Abstract

The invention discloses a sterile line mutant acquisition method which is characterized in that the existing mutant is utilized to be hybridized to other varieties, progeny screening is performed by taking a sterile gene as a marker, and progenies perform backcrossing at the same time; and each backcrossing is subjected to detection with the sterile gene. The method provided by the invention solves the limitation that fertility mutants are long in discovery period and cannot be acquired in batch, and various mutants in different variety types cannot be acquired at the same time when the fertility mutants are acquired before. The method provided by the invention is simple, is liable to operate, avoids the indetermination of EMS mutation, omits the tediousness of a gene editing process, canperform backcross transformation on multiple rice varieties at the same time, greatly improves the acquisition efficiency of the fertility mutants, is beneficial to discovering a third generation intelligent sterile line and accelerates application of a third generation hybrid rice breeding.

Description

technical field [0001] The invention relates to the technical field of bioengineering molecular genetic breeding, in particular to a method for obtaining mutants of sterile lines. Background technique [0002] Rice is the staple food of more than half of the population in China. Therefore, the stability and high yield of rice seriously affect the security of food supply in my country. With the large-scale promotion of hybrid rice, it has made great contributions to ensuring the safety of my country's food supply and ensuring that the total rice output remains unchanged or further increased. [0003] The first generation of hybrid rice in China is a three-line hybrid rice with cytoplasmic male sterile line as a genetic tool; the second generation hybrid rice is a two-line hybrid rice with a photothermosensitive male sterile line as a genetic tool; and currently , China's hybrid rice research has entered the third generation of research, that is, hybrid rice using genetically...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 刘佳音米铁柱张国栋张彦荣李继明邹丹丹邵晓宇
Owner QINGDAO YUANCE GRP CO LTD
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