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Chitosan oligosaccharide prepared by compound enzyme and preparation method thereof

A technology of compound enzyme and chitosan oligosaccharide, applied in the biological field, can solve the problems of non-concentration of polymerization degree, uneven molecular weight distribution of chitosan oligosaccharide, restricted biological activity, etc., achieves the concentration of polymerization degree, shortens the enzymatic hydrolysis time, and is easy to operate. Effect

Active Publication Date: 2018-12-07
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular weight distribution of chitosan oligosaccharides obtained by these methods is not uniform, and the degree of polymerization is not concentrated.
Studies have shown that chitosan molecules with different molecular weights and degrees of polymerization play different roles in biological activities. For example, chitosan with a degree of polymerization of 6-8 has good antibacterial, immune-enhancing, and anti-tumor activities, while chitosan The mixed degree of polymerization of oligosaccharide molecules will seriously limit the biological activity

Method used

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  • Chitosan oligosaccharide prepared by compound enzyme and preparation method thereof
  • Chitosan oligosaccharide prepared by compound enzyme and preparation method thereof
  • Chitosan oligosaccharide prepared by compound enzyme and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1, the chitosan oligosaccharide that a kind of compound enzyme prepares

[0030] The preparation method comprises the following steps:

[0031] S1, the chitosan is dissolved in the acetic acid-sodium acetate buffer solution that the pH value is 4.5, and the concentration is 0.2mol / L, is mixed with mass concentration and is 1% chitosan solution;

[0032] S2. Add pectinase and pepsin to the chitosan solution obtained in step S1 by adding a compound enzyme in a weight ratio of 1:0.6. The mass ratio of the compound enzyme to chitosan is 10%. In a water bath at 50°C Medium enzymatic hydrolysis for 2 hours, after the enzymolysis is completed, place in a boiling water bath, inactivate for 10 minutes, after cooling to room temperature, adjust the pH to 8 with NaOH solution with a mass concentration of 5%, filter, discard the filter residue, and take the filtrate;

[0033] S3. Dialyze the filtrate obtained in S2 with a regenerated cellulose dialysis bag with a molecu...

Embodiment 2

[0034] Embodiment 2, the chitosan oligosaccharide that a kind of composite enzyme prepares

[0035] The difference between embodiment 2 and embodiment 1 is that in step S1, the mass concentration of chitosan solution is 0.5%, and the mass ratio of the quality of compound enzyme and chitosan is 8% in S2 step, and compound enzyme is formed by pectinase and pepsin in a weight ratio of 1:0.1, and refer to Example 1 for other parameters and operations.

Embodiment 3

[0036] Embodiment 3, the chitosan oligosaccharide that a kind of composite enzyme prepares

[0037] The difference between embodiment 3 and embodiment 1 is that in step S1, the mass concentration of chitosan solution is 1.5%, and the mass ratio of the quality of compound enzyme and chitosan is 15% in S2 step, and compound enzyme is formed by pectinase and pepsin in a weight ratio of 1:1, and refer to Example 1 for other parameters and operations.

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Abstract

The invention belongs to the technical field of biology and particularly relates to chitosan oligosaccharide prepared by compound enzyme and a preparation method thereof. The chitosan oligosaccharideprepared by the compound enzyme is characterized in that the preparation method comprises the following steps of: dissolving chitosan in acetic acid-sodium acetate buffering solution, then adding complex enzyme solution into chitosand solution to carry out enzymolysis, after enzymolysis is finished, filtering the obtained enzymolysis solution, then adopting a dialysis method to dialyze filtrate, finally concentrating and drying obtained dialysis solution to obtain the chitosan oligosaccharide. The chitosan oligosaccharide and the preparation method have the beneficial effects that the chitosanoligosaccharide is small in molecular weight, concentrated in degree of polymerization and high in yield, and is more applicable to the fields of medicines, foods, cosmetics and the like; the preparation method is simple in operation and low in material cost and has good industrial promotion and practicability.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a chitosan oligosaccharide prepared by a compound enzyme and a preparation method thereof. Background technique [0002] Oligochitosan is the product of chitosan degraded to a certain extent. Compared with chitosan, oligochitosan has better water solubility and lower viscosity under physiological conditions, which is due to the sugar chain of oligochitosan Shorter and have a large number of free amino groups. The glycoside residue repeating unit of chitosan oligosaccharide contains one amino group and two hydroxyl groups, and is the only positively charged cationic basic amino oligosaccharide in nature. These properties of chitosan oligosaccharides have attracted the attention of more and more researchers. There have been many literatures reporting the biological activities of chitosan oligosaccharides, such as anti-oxidation, anti-inflammation, antagonizing microorgani...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04
CPCC12P19/04C12P19/14
Inventor 苏政权曹华
Owner GUANGDONG PHARMA UNIV
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