Black and ageing specific inducing promoter, engineering vector and application

Abstract

The invention belongs to the technical field of genetic engineering and relates to a black and ageing specific inducing promoter, an engineering vector and an application. The promoter is shown as a nucleotide sequence of SEQ ID NO:1. The engineering vector has a promoter sequence with the nucleotide sequence of SEQ ID NO:2. The invention establishes an SLC1 promoter for driving an IPT1 gene expression vector, so that the specific high expression of the IPT1 gene under a dark condition and in a leaf ageing period can be realized, the acceleration of the ageing process of the leaves under the dark condition can be restrained, and meanwhile, the ageing process of plants caused by age can be restrained, namely, the accelerated ageing process of leaves is restrained under a dark condition while the plant can delay ageing under normal conditions. The black and ageing specific inducing promoter and the engineering vector can be widely applied to the application research on the relevant theory of restraining plant IPT1 gene expression, darkness resistance of plants, ageing resistance, and the like.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a promoter and an engineering carrier with inducible characteristics related to plant leaves, in particular to a dark and senescence-specific inducible promoter, an engineering carrier and applications. Background technique [0002] Senescence is the last stage of plant leaf development. During this process, a series of physiological and biochemical reactions will occur, including chlorophyll degradation, cell structure collapse, and nutrient reuse. Plants will face biological or abiotic factors from the environment during their growth. Stress, such as pests and diseases, long-term low light / darkness, drought, abnormal temperature, etc., after plants suffer from these stresses, they will accelerate the aging process of leaves, and nutrients will be redistributed from aging tissues to fruits or young organs, which will enhance the growth rate of leaves to a certain extent...

AI Technical Summary

Problems solved by technology

[0002] Senescence is the last stage of plant leaf development. During this process, a series of physiological and biochemical reactions will occur, including chlorophyll degradation, cell structure collapse, and nutrient reuse. Plants will face biological or abiotic factors from the environment during their growth. Stress, such as pests and diseases, long-term low light / darkness, drought, abnormal temperature, etc., after plants suffer from these stresses, they will accelerate the aging process of leaves, and nutrients will be redistributed from aging tissues to fruits or young organs, which will enhance the growth rate of leaves to a certain extent. Plant adaptability, therefore, leaf senescence has positive significance for plants; however, abnormal leaf senescence (premature senescence or late senescence) affects crop yield and quality; premature senescence prevents plants from fully completing photosynthesis, and organic matter accumulates and harvests It may cause crop yield reduction and quality degradation; understanding and realizing the artificially controllable leaf senescence process is of great significance in agricultural production;

[0003] Leaf senescence is the result of a combination of internal and external factors, among which weak light or darkness can accelerate the process of leaf senescence, which is also one of the important influencing factors; studies have shown that in the process of plant senescence affected by light, the photoreceptor phyB - The PIF pathway is involved, and plant hormones such as jasmonic acid are also involved in the leaf senescence process induced by low light / darkness; in actual agricultural production, the spacing between plants or rows is small, and the leaves of crops block each other, which is prone to shade avoidance effects , premature senescence and shedding of leaves, affecting yield and quality

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