Roller hollow fiber reactor for plant cell high-density culturing and method for performing high-density culturing on psidium guajava leaf cells

Abstract

The invention discloses a roller hollow fiber reactor for plant cell high-density culturing. The roller hollow fiber reactor comprises a roller, hollow fiber tubes, a culture liquid circulating pipe and driving devices for driving the roller to rotate, wherein the roller is not communicated with the outside; the hollow fiber tubes are fixedly arranged in the roller; at least one hollow fiber tubeis arranged; the culture liquid circulating pipe is communicated with the pipe cavity of the hollow fiber tube; the culture liquid circulating pipe is provided with a culture liquid circulating pump.Correspondingly, the invention also provides a method for performing culturing psidium guajava leaf cells by using the roller hollow fiber reactor. The device disclosed by the invention has the advantages of good mixing performance during cell culturing, low shearing force and energy conservation. In addition, according to the device disclosed by the invention, because the roller is rotated to drive a shell cavity to flow in a flowing body, the tight aggregation and condensation of cells can be greatly reduced and dissolved oxygen of the cells is improved, the mass transfer permeability of a film is improved, cell growth and metabolism are facilitated, and high-density cell culturing is facilitated.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a reactor for cell culture and a method for culturing guava leaf cells. Background technique [0002] Guava plant is a plant with good medicinal and nutritional value. However, when using it for large-scale production of valuable products, there are disadvantages such as long planting cycle, large floor area, long harvesting process, and high cost. The large-scale cultivation of guava plant cells is an effective way to realize high-efficiency, low-cost and large-scale utilization of the valuable components of guava. [0003] Plant cell culture has the characteristics of long cycle, weak cell resistance to shearing, and easy aggregation. The purpose of plant cell-scale culture is to produce valuable natural products, metabolites of cell growth. The design of the plant cell culture reactor and its process method should not only consider the benefits of cell grow...

AI Technical Summary

Problems solved by technology

However, the immobilized plant cell reactor and membrane reactor have the problems of slow mass transfer rate and mass transfer imbalance. When the anchorage-dependent cell culture density increases, the mixing situation becomes poor, and there are cells on the membrane surface or on the wall. Excessive coagulation and a large amount of foam phenomenon will result in insufficient supply of nutrients and oxygen in the cell coagulation environment, resulting in a decrease in the amount of living cells and metabolites, and in severe cases, the culture process will be forced to stop

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