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Preparation method of compound immobilized biological adsorbent

A biosorbent, composite technology, applied in chemical instruments and methods, biological water/sewage treatment, adsorbed water/sewage treatment, etc., can solve the problem of poor chemical stability of immobilized carriers, sensitivity to external extreme environments, and investment costs. Expensive and other problems, to achieve the effect of shortening the immobilization time, adsorption speed, ecological environment protection, and simplifying the preparation process

Inactive Publication Date: 2018-12-07
BEIJING ORIENT LANDSCAPE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the economic and technical problems such as poor firmness of the prior art, easy to be washed away by water flow, poor chemical stability of immobilized carriers, relatively high ecological toxicity, high investment cost, sensitivity to extreme external environments, and poor restoration effect. Insufficient, to provide a composite immobilized biosorbent with high microbial stability, strong stress resistance, fast adsorption speed, clean and pollution-free, and also provide a biosorbent with simple steps, convenient operation, extremely low production cost and excellent product performance Preparation method of composite immobilized biosorbent

Method used

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  • Preparation method of compound immobilized biological adsorbent

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preparation example Construction

[0035] Specifically, the preparation method includes the following steps:

[0036] Step A, under aseptic conditions, inoculate the nitrogen cycle bacteria spore suspension in the sterile modified diatomite and sodium alginate suspension with a mass ratio of 10: (1-15) to form a mixed liquid, and each milliliter of the mixed liquid The inoculum volume in the solution is 0.1-1.0mL, and the mixed solution is obtained after mixing well. Preferably, the concentration of the nitrogen cycle bacteria spore suspension is 1.0×10 6 ~2.0×10 6 individual / mL.

[0037] Step B, under aseptic conditions, add 1 volume of the mixed solution dropwise to 2-8 volumes of sterile CaCl 2 After standing still in the solution, a reaction solution containing modified diatomite-calcium alginate-nitrogen cycle bacteria microspheres is obtained. Preferably, in step B, take 4-5 mL of the mixed solution and add dropwise to 20-40 mL of sterile CaCl 2 solution, the sterile CaCl 2 The concentration of the ...

Embodiment 1

[0062] The preparation method of the composite immobilized biosorbent of the present embodiment comprises the following steps:

[0063] (1) Preparation of sterile modified diatomite and sodium alginate mixture: add 0.10 g of modified diatomite to 5 mL of sodium alginate solution with a concentration of 30 g / L, stir well, and then Sterilize under conditioned conditions for 30 minutes, and finally cool under sterilized conditions for 60 minutes to prepare a sterile modified diatomite and sodium alginate mixture;

[0064] (2) Preparation of modified diatomite-calcium alginate-nitrogen cycling bacteria microspheres: under sterile conditions, inoculate 2.0mL of spores with a concentration of 2.0×10 6 The spore suspension of each / mL was put into the aseptic modified diatomite and sodium alginate mixed solution that 2mL above-mentioned step (1) made, got mixed solution after fully mixing evenly, utilize medical sterile syringe this mixed solution gradually Add dropwise to 16 mL of s...

Embodiment 2

[0072] The preparation method of the composite immobilized biosorbent of the present embodiment comprises the following steps:

[0073] (1) Preparation of sterile modified diatomite and sodium alginate mixture: add 0.10 g of modified diatomite to 2.5 mL of sodium alginate solution with a concentration of 20 g / L, stir well, and then Sterilize at ℃ for 30 minutes, and finally cool under aseptic conditions for 90 minutes to prepare a sterile modified diatomite and sodium alginate mixture;

[0074] (2) Preparation of modified diatomite-calcium alginate-nitrogen cycle bacteria microspheres: under sterile conditions, inoculate 0.2mL of spores with a concentration of 2.0×10 6 The spore suspension of each / mL was put into the aseptic modified diatomite and sodium alginate mixed solution that 2mL above-mentioned step (1) made, got mixed solution after fully mixing evenly, utilize medical sterile syringe this mixed solution gradually Add dropwise to 16 mL of sterile CaCl with a molar co...

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Abstract

The invention relates to a preparation method of a compound immobilized biological adsorbent. The preparation method comprises the following steps: inoculating nitrogen cycle bacteria spore suspensioninto sterile modified diatomite and sodium alginate suspension liquid in a mass ratio of 10 to (1-15) so as to form mixed liquid, adequately and uniformly mixing, so as to obtain a mixed solution, wherein the inoculation amount in each milliliter of the mixed liquid is 0.1mL-1.0mL; dropwise adding 1 volume of the mixed solution into 2-8 volumes of a sterile CaCl2 solution, and standing, so as toobtain reaction liquid containing modified diatomite-calcium alginate-nitrogen cycle bacteria microspheres; and carrying out immobilization culturing on the modified diatomite-calcium alginate-nitrogen cycle bacteria microspheres, so as to obtain the compound immobilized biological adsorbent. The compound immobilized biological adsorbent prepared by virtue of the preparation method has the advantages that the nitrogen and phosphorus removal effects are good, the adsorption capacity is high, the adsorption speed is high, the cost is low, and the compound immobilized biological adsorbent has noenvironmental risk and is economic, environment-friendly, clean and pollution-free and the like.

Description

technical field [0001] The invention relates to the field of biological adsorption and degradation treatment of polluted water bodies, in particular to a preparation method of a composite immobilized biological adsorbent. Background technique [0002] With the rapid development of the economy and society, due to the excessive development and irrational use of water resources, the direct discharge of industrial wastewater, the lagging of urban domestic sewage treatment facilities, and the serious non-standard discharge, a large number of agricultural non-point source pollution and rainwater runoff Without an effective solution, the pollution of the water body will become more and more serious, the pressure on the carrying capacity of the water environment will increase, the self-purification ability of the water body will weaken or even lose the function of the water body, and the water environment will be in crisis. In addition, water pollution has exacerbated the contradict...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/30C02F1/28C02F1/56C02F3/30
CPCB01J20/046B01J20/14B01J20/24C02F1/288C02F1/56C02F3/302
Inventor 陈国敏樊蓓莉崔晨晨
Owner BEIJING ORIENT LANDSCAPE
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