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Application of Glycine tabacina (Labill.) Benth or extract thereof to preparation of drugs used for treating and/or preventing arthritis

An extract, tobacco bean technology, applied in the field of medicine, can solve the problems such as prevention and treatment of arthritis that have not yet been seen with tobacco bean or its extract, and achieve the effect of reducing bone degeneration and articular cartilage destruction, inhibiting oxidative stress, and reducing expression. Effect

Active Publication Date: 2018-12-07
UNIVERSITY OF MACAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the prevention and treatment of arthritis by tobacco beans or their extracts

Method used

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  • Application of Glycine tabacina (Labill.) Benth or extract thereof to preparation of drugs used for treating and/or preventing arthritis
  • Application of Glycine tabacina (Labill.) Benth or extract thereof to preparation of drugs used for treating and/or preventing arthritis
  • Application of Glycine tabacina (Labill.) Benth or extract thereof to preparation of drugs used for treating and/or preventing arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Preparation and Characterization of Tobacco Bean Extract of the Present Invention

[0049] After crushing the tobacco root, put it in an extraction bottle, add 75% or 85% or 95% ethanol at a ratio of 1:10, and extract in an ultrasonic bath for 30 minutes at 30°C, 40°C, or 50°C. Repeat the extraction 3 times, combine the filtrates, and concentrate to obtain the ethanol extract, which is the tobacco bean extract of the present invention. The extraction rate and main component content under different extraction conditions are shown in Table 1.

[0050] Table 1

[0051]

[0052] Use HPLC to detect the above 9 extracts, HPLC conditions: 0-30min, 40-100% MeOH, 60-0% H 2 O; 30-40min, 100% MeOH, 0% H 2 O. The detection wavelength is 280nm. Such as figure 1 As shown, within the range of conditions for the preparation of the provided tobacco bean extract, the components of the extract are highly similar, indicating that the tobacco bean extract of the present i...

experiment example 1

[0058] Experimental example 1 The tobacco bean extract of the present invention significantly relieves the symptoms of arthritis in rats

[0059] 1. Experimental method

[0060] Thirty male SPF-grade Wistar rats (350-420g) were purchased from the Experimental Animal Center, Faculty of Health Sciences, University of Macau (animal breeding license number: UMARE-014-2016). Animal experiments were carried out in strict accordance with the requirements of the Experimental Animal Ethics Committee of the University of Macau. Glycine tabacina extract (GTE) was prepared according to Example 1. The positive drug, indomethacin hydrochloride, was purchased from Sigma Corporation in the United States, prepared into a 1 mg / ml indomethacin stock solution with 0.9% normal saline, aliquoted, and stored in a -80°C refrigerator. Both Collagen II and incomplete Freund's adjuvant were purchased from Chondrex Company in the United States. When used, the two were uniformly mixed in equal volumes t...

experiment example 2

[0074] Experimental example 2 The tobacco bean extract of the present invention significantly reduces bone degeneration and articular cartilage destruction in arthritic rats

[0075] 1. Experimental method

[0076] The 10% neutral formalin fixative used in the experiment was produced by Nanchang Yulu Experimental Equipment Co., Ltd. Solid paraffin was purchased from Shanghai Yiyang Instrument Co., Ltd. Hematoxylin-eosin staining solution and Safranin-O staining solution were purchased from Shanghai Jinsui Biotechnology Co., Ltd. After 30 days of modeling, the rats were sacrificed, the left hind paw was separated, peeled and depilated, fixed overnight in 10% formalin solution, and fully decalcified with compound decalcification solution (8ml HCl+5ml glacial acetic acid+10g water Salicylic acid+H 2 O to 100ml), for routine pathological examination.

[0077] 2. Experimental design

[0078] Before the experiment, the rats were randomly divided into 6 groups: control group, mo...

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Abstract

The invention discloses an extract of Glycine tabacina (Labill.) Benth. The extract contains isoflavones, triterpenoids, scoparone and vanillic acid. The results of pharmacodynamic experiments show that the extract of Glycine tabacina (Labill.) Benth has obvious therapeutic effect on arthritis, can significantly reduce the degenerative changes of bones and destroy to cartilago articularis, decreases the expression of pro-inflammatory cytokines, inhibits oxidative stress, and provides a novel medication option for clinical treatment of arthritis.

Description

technical field [0001] The invention relates to the use of tobacco beans or its extracts in the preparation of medicines for treating and / or preventing arthritis, which belongs to the field of medicine. Background technique [0002] Arthritis is a multisystem autoimmune disease dominated by chronic inflammatory response and joint destruction. Accumulating evidence indicates that inflammation can lead to disturbance of bone homeostasis. At present, arthritis is mainly treated with hormones, non-steroidal anti-inflammatory drugs and cytotoxic drugs, but these drugs have many side effects, so that they cannot be taken for a long time, and the destruction of bone tissue is still a frequent and serious problem in the treatment of many arthritis patients. The problem. Therefore, it is still urgent to develop new high-efficiency and low-toxicity drugs for the prevention and treatment of inflammation and joint damage in arthritis. Natural products have the characteristics of wide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P19/02A61P29/00A61K125/00A61K127/00A61K135/00
CPCA61K36/48A61K2236/333A61K2236/39
Inventor 何承伟王恺梁叶儿陈美婉李鹏万建波王一涛
Owner UNIVERSITY OF MACAU
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