Selected-layer blood flow speckle imaging device and method based on sliced-layer light

Abstract

The invention discloses a selected-layer blood flow speckle imaging device and method based on sliced-layer light. The device comprises a laser light source, a beam expander, a beam splitter, a firstvarifocus lens, a second varifocus lens, a first cylindrical mirror, a second cylindrical mirror, a motor, an autofocus lens, an image acquisition camera and a processor; a laser beam output by the laser light source forms a first linear beam and a second linear beam through the beam expander and the beam splitter, the first linear beam sequentially passes through the first varifocus lens and thefirst cylindrical mirror and forms the first sliced-layer light, the second linear beam sequentially passes through the second varifocus lens and the second cylindrical mirror and forms the second sliced-layer light, and the first sliced-layer light and the second sliced-layer light enter the image acquisition camera through the autofocus lens after being absorbed by a sample to be tested. By means of the device, the two beams of sliced-layer light coincide at the trisection points of the sample to be tested to collect information of different selected layers of the sample to be tested; by means of the device, the complex fluorescent dyeing and detection operation is avoided, and the imaging operation is simple.

Description

technical field [0001] The present invention relates to the technical field of blood flow speckle imaging, in particular to a layer-selected blood flow speckle imaging device and method based on slice light. Background technique [0002] In terms of blood flow speckle imaging, there is currently an RFLSI Pro laser speckle blood flow imager from Beijing Giandel Technology Co., Ltd. that can monitor two-dimensional blood flow images at the micron level in real time, which can be measured and displayed intuitively. The moorFLPI-2 zoom laser speckle blood flow imaging system for tissue blood flow distribution, and the moorFLPI panoramic blood flow perfusion imaging system that can provide video rate up to 25 frames per second of blood flow changes in microvessels. [0003] In terms of slice light application imaging, it is now possible to detect senescent cells without complicated fluorescent labeling operations and detection processes. In the patent document with the publicati...

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Problems solved by technology

In the patent document with the publication number CN 106520535A, Jiangxi Gujiu Metal Products Co., Ltd. discloses a label-free cell detection device based on light sheet illumination. This device avoids complicated operations such as fluorescent dyeing and detection, and makes the operation simple. Only single-beam scanning is used, which is lacking in imaging quality; in the patent document with the publication number CN 104155274A, Huazhong University of Science and Technology discloses a micro-scanning imaging method and microscope for double-beam light sheet photography, which can quickly scan samples Scanning speeds up the three-dimensional imaging speed, but this technical solution still relies on fluorescence imaging like traditional light sheet illumination imaging

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