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Method for preparing probiotic microcapsule by utilizing spray drying

A technology of probiotics and microcapsules, applied in the field of microorganisms, can solve the problem of high bacterial mortality, achieve the effect of ensuring the number of viable bacteria and increasing tolerance

Inactive Publication Date: 2018-12-07
广州富诺营养科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a method for preparing probiotic microcapsules by spray drying, which solves the problem of high bacterial mortality due to the high temperature in the dryer, and ensures the number of live bacteria in the bacterial powder

Method used

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  • Method for preparing probiotic microcapsule by utilizing spray drying

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for preparing probiotic microcapsules by spray drying, comprising the following steps:

[0026] 1. Preparation of medium: medium is made of the following components in parts by weight: 5 parts of yeast powder, 10 parts of peptone, 20 parts of glucose, 5 parts of sodium acetate trihydrate, 0.5 part of magnesium sulfate heptahydrate, 2 parts of dipotassium hydrogen phosphate 2 parts of diammonium citrate, 10 parts of meat extract, 0.05 parts of manganese sulfate tetrahydrate, 801 parts of Tween, 1000 parts of purified water; yeast powder, peptone, glucose, sodium acetate trihydrate, magnesium sulfate heptahydrate , dipotassium hydrogen phosphate, diammonium citrate, meat extract 10, manganese sulfate tetrahydrate, and Tween-80 are poured into pure water according to the ratio and heated to dissolve. Sterilize under conditioned conditions for 20 minutes and set aside;

[0027] 2. Activation of the bacteria: In a sterile environment, restore the bacteria from the ...

Embodiment 2

[0039] The basic steps of this example are the same as those in Example 1, except that in Step 3, culture is placed in a 37°C incubator, and after 12 hours of incubation, the petri dish is placed in a 70°C constant temperature water bath for 5 minutes.

Embodiment 3

[0041] The basic steps of this example are the same as those in Example 1, except that in Step 3, culture is placed in a 37°C incubator, and after 13 hours of culture, the petri dish is placed in a 80°C constant temperature water bath for 6 minutes.

[0042] The comparison table between the embodiment of the present invention and the existing preparation method under the same experimental conditions:

[0043]

[0044] As can be seen from the above table, compared with the existing preparation method, the product of the present invention can effectively increase the number of live bacteria in the bacterial powder.

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Abstract

The invention discloses a method for preparing a probiotic microcapsule by utilizing spray drying. The method comprises the following steps:1, preparation of a culture medium; 2, activation of thallus; 3, proliferation and heat shock of the thallus: inoculating the activated thallus according to 1 percent of inoculation quantity, putting the activated thallus in a 37 DEG C-constant temperature boxand performing culturing; and putting a culture dish into a constant-temperature water bath pot of 60 DEG C to 80 DEG C after culturing is carried out for 11 hours to 13 hours, and then performing water bath for 4 minutes to 6 minutes; 4, concentration of the thallus: pouring culture liquid after heat shock into a sterilizing tube, putting the sterilizing tube into a centrifugal machine, settingrotation speed of the centrifugal machine as 6000r / min, and ensuring that the temperature is 4 DEG C and the centrifugation time is 10 minutes; 5, preparation of mixed suspension; 6, spray drying; and7, performing packaging. The invention relates to the technical field of microorganisms. By adopting the method for preparing the probiotic microcapsule by utilizing the spray drying, the problem ofa high death ratio of the thallus caused by a higher temperature in a drying machine is solved, and the viable count of probiotic powder is ensured.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for preparing probiotic microcapsules by spray drying. Background technique [0002] Probiotics, also known as prebiotics, probiotics, active bacteria, and microecological preparations, refer to those live bacterial products that maintain the balance of human flora and have beneficial effects on human health or contain bacterial components and metabolites. Dead bacteria products. The types of probiotics currently used in humans mainly include Lactobacillus, Bifidobacterium, Enterococcus, Lactococcus, Bacillus subtilis, Escherichia coli, Bacillus cereus, Bacillus licheniformis, Streptococcus thermophilus, Clostridium butyricum and Yeast, etc., but traditionally people use mainly two genera of Bifidobacterium and Lactobacillus. However, the most critical problem of probiotic preparations is that the number of viable bacteria in the product decreases rapidly. Accor...

Claims

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Application Information

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IPC IPC(8): A23L33/135C12N1/00
CPCA23L33/135C12N1/00
Inventor 程彦
Owner 广州富诺营养科技有限公司
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