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Detection kit for determining serum amyloid A protein with latex-enhanced immunoturbidimetric assay

A serum starch, immune turbidimetric technology, applied in the field of detection, can solve the problems of protein activity decline, reduce the sensitivity of reagents, etc., and achieve the effect of maintaining stable activity

Inactive Publication Date: 2018-11-23
浙江伊利康生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the protein-microspheres prepared by this method, the protein and the latex microspheres are in direct contact, and the surface of the latex microspheres will affect the structure of the antibody, which will reduce the activity of the protein and eventually reduce the sensitivity of the reagent.

Method used

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  • Detection kit for determining serum amyloid A protein with latex-enhanced immunoturbidimetric assay
  • Detection kit for determining serum amyloid A protein with latex-enhanced immunoturbidimetric assay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The present invention provides a detection kit for the determination of serum amyloid A by latex-enhanced immune turbidimetry, wherein the R1 reagent and the R2 reagent are respectively placed in the kit.

[0022] The present invention also provides a kind of R1 reagent, and its raw material formula is as follows:

[0023] HEPES buffer 50mM

[0024] PEG8000 10g / L

[0025] NaN3 1g / L

[0026] Gelatin 1g / L

[0027] BSA 2g / L

[0028] NaCl 1.3g / L

[0029] TritonX-100 0.2%

[0030] PH=7.4

[0031] The present invention also provides a kind of R2 reagent, the formula is as follows:

[0032] Latex particles coated with SAA antibody 0.4%

[0033] HEPES buffer 50mM

[0034] PEG8000 10g / L

[0035] NaN3 1g / L

[0036] Glycerin 1%

[0037] Sorbitol 1.5%

[0038] Peptone 1.2%

[0039] BSA 1g / L

[0040] NaCl 2.9g / L

[0041] TritonX-100 0.2%

[0042] PH=7.0

[0043] The present invention also provides a preparation method of latex particles coated with serum amyloid A ...

Embodiment 2

[0050] The present invention provides a detection kit for the determination of serum amyloid A by latex-enhanced immune turbidimetry, wherein the R1 reagent and the R2 reagent are respectively placed in the kit.

[0051] The present invention also provides a kind of R1 reagent, and its raw material formula is as follows:

[0052] Tris-HCl buffer 75mM

[0053] PEG8000 15g / L

[0054] NaN3 1.5g / L

[0055]Gelatin 2g / L

[0056] BSA 2g / L

[0057] NaCl 1.5g / L

[0058] TritonX-100 0.2%

[0059] PH=7.5

[0060] The present invention also provides a kind of R2 reagent, the formula is as follows:

[0061] Latex particles coated with SAA antibody 0.5%

[0062] Tris-HCl buffer 75mM

[0063] PEG8000 10g / L

[0064] NaN3 1g / L

[0065] Glycerin 2%

[0066] Sorbitol 1.5%

[0067] Peptone 1.5%

[0068] BSA 1g / L

[0069] NaCl 2.9g / L

[0070] TritonX-100 0.2%

[0071] PH=7.0

[0072] The present invention also provides a preparation method of latex particles coated with serum amy...

Embodiment 3

[0079] The present invention provides a detection kit for the determination of serum amyloid A by latex-enhanced immune turbidimetry, wherein the R1 reagent and the R2 reagent are respectively placed in the kit.

[0080] The present invention also provides a kind of R1 reagent, and its raw material formula is as follows:

[0081] PBS buffer solution 75mM

[0082] PEG6000 15g / L

[0083] Proclin-300 0.1%

[0084] Gelatin 2g / L

[0085] BSA 2g / L

[0086] NaCl 1.5g / L

[0087] TWeen-20 0.2%

[0088] PH=7.5

[0089] The present invention also provides a kind of R2 reagent, the formula is as follows:

[0090] Latex particles coated with SAA antibody 0.3%

[0091] PBS buffer solution 75mM

[0092] PEG6000 10g / L

[0093] Proclin-300 0.1%

[0094] Glycerin 2%

[0095] Sorbitol 1.5%

[0096] Peptone 2%

[0097] BSA 1.5g / L

[0098] NaCl 2.9g / L

[0099] TWeen-20 0.1%

[0100] PH=7.0

[0101] The present invention also provides a preparation method of latex particles coated...

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Abstract

The invention discloses a detection kit for determining serum amyloid A protein with latex-enhanced immunoturbidimetric assay, which contains R1 reagent and R2 reagent respectively. The detection kitfor determining serum amyloid A protein with latex-enhanced immunoturbidimetric assay adopts a new latex-enhanced immunoturbidimetric assay, wherein NHS reacts with carboxyl on microspheres to produceintermediate activated ester under the presence of EDAC to make the SAA detection kit more sensitive and stable; at the same time, Tween20 or Tergitol NP9 or other nonionic surfactant is added into the reaction system to prevent the microspheres from affecting the protein structure, so as to maintain activity and stability of the protein to the greatest extent and avoid agglutination phenomenon.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a detection kit for the determination of serum amyloid A by latex-enhanced immune turbidimetry. Background technique [0002] Serum amyloid A (serum amyloid A, SAA) is an acute time-limited response protein, which belongs to the heterogeneous class of proteins in the apolipoprotein family, with a relative molecular weight of about 12 000. In an acute time-limited response, SAA is synthesized in the liver by activated macrophages and fibroblasts stimulated by IL-1, IL-6 and TNF, and can be increased to 100-1000 times the initial concentration, but the half-life is extremely Short, only about 50 minutes. Serum amyloid A is related to high-density lipoprotein (HDL), which regulates HDL metabolism during inflammation. A particularly important property of serum amyloid A is the deposition of its degradation products in various organs as amyloid A (AA) fibrils, a serious complicati...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543G01N21/82
CPCG01N33/6893G01N21/82G01N33/54313G01N2333/4709
Inventor 王贤理池万余卢强贾刚
Owner 浙江伊利康生物技术有限公司
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