Method and system for removing cover slip

A cover glass and slide glass technology, which is applied in biochemical equipment and methods, microbial measurement/inspection, and specific-purpose bioreactors/fermenters, etc. Problems such as not being discovered in time can achieve the effect of improving stability, improving operating efficiency and reducing errors

Inactive Publication Date: 2018-11-23
上海兰卫医学检验所股份有限公司
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  • Abstract
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Problems solved by technology

In the current HER2 standard operating procedure, the general operation for removing the coverslip is "put the slide in room temperature washing solution and shake slightly until the cover slip is confirmed to fall off", but in the actual operation process, the coverslip is difficult to remove. Detachment from the slide, and there is a phenomenon that the slide is detached but not detected in time

Method used

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  • Method and system for removing cover slip
  • Method and system for removing cover slip
  • Method and system for removing cover slip

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Embodiment Construction

[0026] The implementation of the present invention is described below through specific examples and in conjunction with the accompanying drawings, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific examples, and various modifications and changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention.

[0027] figure 1 It is a flowchart of steps of a method for removing a cover glass of the present invention. Such as figure 1 Shown, a kind of method for removing cover glass of the present invention comprises the steps:

[0028] Step S1, immersing the coverslip in the washing solution for a preset time. In the specific embodiment of the present invention, the cover glass can be soaked in...

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Abstract

The invention discloses a method and a system for removing a cover slip. The method comprises the following steps: step S1, immersing the cover slip in a washing solution for a preset time; step S2, selecting an object in regular edges, and fixing one side of the cover slip; step S3, utilizing an object of which the head end is thinner and inserting the head end of the object in the lower part ofthe other side of the cover slip, and controlling the object of which the head end is thinner to slowly separate the cover slip from a sample, thus removing the cover slip. According to the method disclosed by the invention, a cover slip removing technology which is capable of rapidly and easily observing a result can be realized.

Description

technical field [0001] The present invention relates to the technical field of fluorescence in situ hybridization (Fluorescence in situ hybridization, FISH), in particular to a method and system for removing a cover glass. Background technique [0002] Fluorescence in situ hybridization (FISH) technology can be used to directly observe specific nucleic acids in cells in vitro. When using the FISH platform to detect HER2 gene amplification, the paraffin-embedded tissue sections are generally aged first, that is, pre- treatment; secondly, the DNA is denatured into a single strand, and then hybridized with the probe. Since the probe is fluorescent, the hybridization probe and target DNA can be clearly observed under a fluorescence microscope under appropriate excitation light irradiation. [0003] When applied to the observation tool fluorescence microscope, in most cases, a cover glass is used for detailed observation later. In the experimental operation of detecting HER2 ge...

Claims

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Application Information

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IPC IPC(8): C12Q1/6841C12M1/00
CPCC12Q1/6841
Inventor 贺双意朱剑董昭李昂
Owner 上海兰卫医学检验所股份有限公司
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