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Pantoea alhagi as well as bacterium agent and application thereof

A technology of Pantoea elegans and camel, which is applied to Pantoea mellifera and its inoculants and application fields, can solve problems such as plant drought, and achieve the effects of simple production process, increased yield and balanced maintenance.

Active Publication Date: 2018-11-23
NANJING SHINEKING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: In order to solve the problem of plant drought, the present invention provides a kind of Pantoea camelii, the present invention further provides the antimicrobial agent with Pantoea camelii as active microorganism, the present invention further provides Pantoea camelii and The application of its bacterial agent in enhancing the drought resistance ability of crops

Method used

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  • Pantoea alhagi as well as bacterium agent and application thereof
  • Pantoea alhagi as well as bacterium agent and application thereof
  • Pantoea alhagi as well as bacterium agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Screening and Identification of Pantoea camelis XK-11

[0027] Pantoea camelina XK-11 is a strain selected by the inventor from the soil of the Grand Canyon in western Zhejiang (based on polysaccharide production). The screening process is as follows:

[0028] Dissolve the collected soil samples in sterile water, shake and mix well. The sample solution was serially diluted, taking 10 -4 、10 -5 、10 -6The dilutions of three concentration gradients were evenly spread on the solid medium (medium components: sucrose 20g / L, peptone 5g / L, beef extract 1g / L, NaCl 5g / L, agar 20g / L). Place them in a constant temperature incubator at 30°C for 48 hours. During this period, check the plate every day to observe the characteristics of the colonies, and use sticky colonies as the screening target. A single colony capable of producing high-viscosity exopolysaccharides was picked and purified by streaking on solid culture hard plates.

[0029] Inoculate the single colony o...

Embodiment 2

[0035] Embodiment 2 Pantoea camelidum XK-11 bacterial agent

[0036] The Pantoea alhagi XK-11 stored on a slant at 4°C was activated and inoculated on the eggplant bottle medium (ie, the activation medium). The activation temperature was 32°C and the activation time was 16 hours. Pick a large number of strains from the eggplant bottle and inoculate them in the fermentation medium. The ventilation rate of sterile air in the fermenter is 0.2vvm, the stirring speed is 200rpm, and cultured at 30°C for 48h to obtain the fermentation liquid. The number of bacteria in the bacterial agent Up to 5×10 9 CFU / g or more.

[0037] The activation medium is LB medium, and the components of the fermentation medium are: glucose 40g / L, beef extract 8g / L, NaCl 6g / L, peptone 10g / L, pH 7.0.

Embodiment 3

[0038] Example 3 Determination of the ability of Pantoea camelini XK-11 to produce extracellular polysaccharides

[0039] The exopolysaccharide content in the fermentation broth of Example 2 was quantitatively detected by the phenol sulfuric acid method, and the exopolysaccharide production amount of Pantoea camelini XK-11 was calculated according to the standard curve prepared from anhydrous glucose. The results showed that the production of exopolysaccharide of Pantoea camelid XK-11 reached 5g / L.

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Abstract

The invention discloses pantoea alhagi. A classified name is pantoea alhagi and a strain number is XK-11; the pantoea alhagi is preserved in China General Microbiological Culture Collection Center (CGMCC) and has a preservation number of CGMCC NO. 15525; the invention further discloses application of the pantoea alhagi to enhancement of drought resistance of crops. The invention discloses a bacterium agent taking the pantoea alhagi as an active microorganism, and further discloses application of the bacterium agent to the enhancement of the drought resistance of the crops. The pantoea alhagi XK-11 can be adhered to a plant root system and also can enter plants to be symbiotic with the plants; the absorption of the plants on external nutrient elements is improved, a metabolic system of theplants is improved and a water potential in the plants is kept, so that the growth and development of the plants are promoted and the yield is improved; meanwhile, the drought resistance capability ofthe plants is enhanced. The pantoea alhagi has extremely important meaning and application value in the aspects of sufficiently expressing the ecological fertility of soil, keeping an agricultural ecological environment balanced and the like.

Description

technical field [0001] The invention relates to the field of microbial bacterial agents, in particular to Pantoea camelina, bacterial agents and applications thereof. Background technique [0002] Soil aridification is one of the most important abiotic stress factors affecting modern agricultural production. The impact of drought on crop yields in the world ranks first among other abiotic stresses. Its degree of damage to plants is second to that of other natural The first disaster. Most of China belongs to the Asian monsoon region. Drought disasters have the characteristics of seasonality, universality, continuity and regionality. The semi-arid and arid areas account for 52% of my country's land area. Even non-arid areas face seasonal drought and uneven precipitation. [0003] Since the first use of microbial agents in the world in 1888, it has been widely promoted and far-reaching in terms of quantity and quality. Due to the rapid reproduction of microorganisms, it can ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C05F11/08C12R1/01
CPCC05F11/08C12N1/20C12P19/04C12N1/205C12R2001/01
Inventor 许宗奇冯小海周哲詹伊婧徐虹
Owner NANJING SHINEKING BIOTECH CO LTD
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