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Application of active octapeptide in aspect of protecting sensory hair cells

A sensory hair cell, active technology, applied in the application field of active octapeptide in the protection of sensory hair cells, to achieve broad market prospects, improve damage, and less toxic and side effects

Active Publication Date: 2018-11-23
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is no effective drug to cure the sensorineural deafness caused by aminoglycoside antibiotics.

Method used

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  • Application of active octapeptide in aspect of protecting sensory hair cells
  • Application of active octapeptide in aspect of protecting sensory hair cells
  • Application of active octapeptide in aspect of protecting sensory hair cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Protective effect of 60 μg / ml active octapeptide sample on zebrafish sensory hair cells

[0039] experimental method

[0040] 1. Zebrafish sample pretreatment:

[0041] Use healthy and sexually mature AB zebrafish, put them into a mating tank at a ratio of 1:1 for males and females, place a partition in the middle, and place them in a dark environment. Remove the partition before turning on the light the next day, and light stimulate it to ovulate. The adult fish are fished out half an hour after ovulation, and the ovulation time is controlled within half an hour to reduce the difference in development time between embryos. Collect the fertilized eggs and rinse them three times with new zebrafish embryo culture water to disinfect and clean the fertilized eggs; then move the fertilized eggs into clean zebrafish embryo culture water, and add 0.2ppm methylene blue to the culture water, 28 ℃, 14h light / 10h dark cycle, light-controlled culture, about 1 / 3 of the ...

Embodiment 2

[0050]Example 2: Protective effect of 80 μg / ml active octapeptide sample on zebrafish sensory hair cells

[0051] The pretreatment of zebrafish samples, the labeling of zebrafish sensory hair cells and the statistics of the number of sensory hair cells are the same as in Example 1.

[0052] Active octapeptide treatment:

[0053] The active octapeptide whose amino acid sequence is shown as SEQ ID NO.1 is synthesized by artificial synthesis, and the octapeptide is synthesized by the Fmoc solid-phase synthesis method described by Liu Zhennan [4] synthesis.

[0054] Set up a control group, a gentamicin modeling group, and an active octapeptide treatment group. Remove the culture water in the 6-well plate, add 6ml culture water solution to the control group; add gentamicin with a final concentration of 150M in the 6ml culture water of the gentamicin modeling group; 150 M of gentamicin and 80 μg / ml of active octapeptide. The zebrafish embryos of the above groups were placed in a...

Embodiment 3

[0056] Example 3: Protective effect of 90 μg / ml active octapeptide sample on zebrafish sensory hair cells

[0057] The pretreatment of zebrafish samples, the labeling of zebrafish sensory hair cells and the statistics of the number of sensory hair cells are the same as in Example 1.

[0058] Active octapeptide treatment:

[0059] The active octapeptide whose amino acid sequence is shown as SEQ ID NO.1 is synthesized by artificial synthesis, and the octapeptide is synthesized by the Fmoc solid-phase synthesis method described by Liu Zhennan [4] synthesis.

[0060] Set up a control group, a gentamicin modeling group, and an active octapeptide treatment group. Remove the culture water in the 6-well plate, add 6ml culture water solution to the control group; add gentamicin with a final concentration of 150M to the 6ml culture water of the gentamicin modeling group; add a final concentration of 150M to the 6ml culture water of the active octapeptide treatment group 150M gentamic...

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Abstract

The invention relates to application of active octapeptide in the aspect of protecting sensory hair cells. According to the application of the active octapeptide in the aspect of protecting the sensory hair cells, the amino acid sequence of the octapeptide is as shown in SEQ ID NO.1. The application of the active octapeptide in the aspect of protecting the sensory hair cells has the advantages that active peptide containing 8 amino acid residues is used in sensory hair cell damage caused by aminoglycoside antibiotics; and after the detection, the condition that the peptide compound can play arole in protecting the sensory hair cells by decreasing the active oxygen content in a body is found for the first time, the damage of ototoxic drugs to the sensory hair cells is ameliorated, the toxic and side effects are small, and the active octapeptide is a hearing protection drug with a good development prospect and has a wide market prospect.

Description

technical field [0001] The invention relates to the application of an active octapeptide in protecting sensory hair cells, and belongs to the technical field of biomedicine. Background technique [0002] Aminoglycoside antibiotics have good water solubility, stable chemical properties, broad antibacterial spectrum, strong antibacterial ability, and good excretion, so they are widely used in clinical practice. They have been effective drugs against Gram-negative and positive bacteria for more than half a century. However, these drugs are highly ototoxic, killing sensory hair cells in the inner ear of mammals. [0003] Sensory hair cells are a kind of auditory receptor cells, which can convert external sound stimuli into neural electrical signals, thereby producing hearing. But in the mammalian auditory system, sensory hair cells, as a terminally differentiated cell, cannot regenerate. Therefore, there is no effective drug to cure the sensorineural deafness caused by aminogl...

Claims

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Application Information

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IPC IPC(8): A61K38/08A61P27/16A61P39/06A23L33/18
CPCA23V2002/00A61K38/08A23L33/18A61P27/16A61P39/06A23V2200/30A23V2200/322A23V2250/55
Inventor 孙晨张姗姗刘可春韩利文张云王雪
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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