Use of catechin and epicatechin compounds for up-regulation of expression level of microRNA-126

A technology of expression levels and compounds, applied in the direction of digestive system, drug combination, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2018-11-23
CATCH BIO SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, there is no report about the use of catechins and epicatechins in the preparation of drugs that up-regulate the expression level of MicroRNA-126

Method used

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  • Use of catechin and epicatechin compounds for up-regulation of expression level of microRNA-126
  • Use of catechin and epicatechin compounds for up-regulation of expression level of microRNA-126
  • Use of catechin and epicatechin compounds for up-regulation of expression level of microRNA-126

Examples

Experimental program
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Effect test

experiment example 1

[0050] Experimental example 1 Effects of catechins and epicatechin compounds on the expression level of MicroRNA-126 in congenitally hypertensive rats and their effect on reducing hypertension

[0051] 1. Purpose of the experiment

[0052] To study the effects of catechins and epicatechins on the expression level of MicroRNA-126 in congenitally hypertensive rats and their effect on reducing hypertension.

[0053] 2. Experimental method

[0054] 2.1 Experimental materials

[0055] Catechin, catechin gallate, gallocatechin, gallocatechin gallate, epicatechin, epicatechin gallate, epigallocatechin and epigallocatechin gallate The esters are all obtained by purchasing, with a purity>98% (HPLC detection);

[0056] 75 congenitally hypertensive rats SHR, over 10 weeks old and weighing over 300g, were purchased from Shanghai Slac Experimental Animal Center;

[0057] Chloral hydrate (ie: chloral hydrate); absolute ethanol; sterile cotton; RNA extraction kit; cDNA synthesis kit; Ta...

experiment example 2

[0101] Experimental example 2 The therapeutic effect of catechins and epicatechin compounds on rats with inflammatory bowel disease and the effect on the expression level of MicroRNA-126

[0102] 1. Purpose of the experiment

[0103] To study the therapeutic effect of catechins and epicatechins on 2,4,6-trinitrobenzenesulfonic acid (trinitrobenzenesulfonic acid, TNBS)-induced rat inflammatory bowel disease and the expression level of MicroRNA-126 Influence.

[0104] 2. Experimental method

[0105] 2.1 Experimental materials

[0106] Gallocatechin, epigallocatechin, and epigallocatechin gallate are all purchased, with a purity >98% (HPLC detection);

[0107] 50 Wistar rats, half male and half female, weighing (200±20) g, were purchased from Shanghai Slac Experimental Animal Center;

[0108] 5% TNBS aqueous solution was purchased from Sigma Company.

[0109] 2.2 Modeling method

[0110] After 7 days of adaptive feeding, 50 rats were randomly divided into blank control gr...

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Abstract

The invention belongs to the field of medicines or health products, and particularly relates to the use of catechin and epicatechin compounds in the preparation of drugs for up-regulating the expression level of microRNA-126. As found by researches, catechin, catechin gallate, gallocatechin, (-)-gallocatechin gallate, epicatechin, epicatechin gallate, epigallocatechin and epigallocatechin gallatecan up-regulate the expression level of the microRNA-126, and can be used for treating tumors, asthma, inflammatory bowel disease, vascular inflammation, hypertension, type 2 diabetes mellitus, diabetic nephropathy or diabetic retinopathy.

Description

technical field [0001] The invention belongs to the field of medicines or health care products, and in particular relates to the use of catechins and epicatechins in the preparation of medicines for up-regulating the expression level of MicroRNA-126. Background technique [0002] MicroRNA is a kind of non-coding single-stranded small molecule RNA with a length of about 19-23 nucleotides. Mature MicroRNA can complement and pair with target mRNA molecules to induce target mRNA degradation or translation inhibition, thereby exerting the function of regulating gene expression. Studies in recent years have shown that MicroRNA is closely related to the occurrence and development of tumors, cardiovascular diseases, diabetes, human genetic diseases and even major diseases of the nervous system. MicroRNA has tissue specificity. Among all MicroRNAs, MicroRNA-126 is an endothelial-specific MicroRNA, which can promote angiogenesis and maintain the integrity of blood vessels. Studies ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/353A61P35/00A61P11/06A61P1/00A61P29/00A61P9/12A61P3/10A61P13/12A61P27/02
CPCA61K31/353
Inventor 温尧林钱炳俊
Owner CATCH BIO SCI & TECH
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