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Identification method for three polygonatum sibiricum with legal origin

A kind of Jiyuan Polygonatum and statutory technology, applied in the field of identification of Polygonatum chemical components, can solve problems such as identification of difficult traits, identification of different varieties of medicinal materials, large differences in diosgenin content, etc.

Inactive Publication Date: 2018-11-20
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Polygonatum plant species has been established [6] The HPLC fingerprints of the HPLC fingerprints and the HPLC fingerprints of the acid hydrolyzate of Polygonatum from Zhejiang [7] , and reported that the content of diosgenin in Polygonatum plants from different origins varies greatly [6] However, whether there are differences in the chemical composition of the three legal sources of Polygonatum recorded in the Pharmacopoeia has not yet been reported.
In addition, in the medicinal material market, most of the commercially available Polygonatum medicinal materials are in the form of flakes, and it is difficult to distinguish and identify different varieties of medicinal materials by means of character identification and microscopy.

Method used

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  • Identification method for three polygonatum sibiricum with legal origin
  • Identification method for three polygonatum sibiricum with legal origin
  • Identification method for three polygonatum sibiricum with legal origin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 117

[0051] Example 1 Identification of 17 batches of Polygonatum medicinal materials

[0052] 1 Instruments and reagents

[0053] 1.1 Instrument

[0054] Ultimate 3000 ultra-high performance liquid chromatography series Q Exactive quadrupole-electrostatic field orbitrap high-resolution mass spectrometer (Thermo Fisher Scientific, the United States), equipped with CBM-20A system controller, LC-20AD quaternary pump, SIL- 20A autosampler, CTO-20A column oven and SPD-M20A PDA detector; ultrasonic cleaning machine (Ningbo Xinzhi Biotechnology Co., Ltd.); DK-S24 electric constant temperature water bath (Shanghai Jinghong Experimental Equipment Co., Ltd.) ; N-11000-WD rotary evaporator (Shanghai Airon Instrument Co., Ltd.); 1 / 10,000 electronic analytical balance (Germany Sartorius Company); HR / 16M high-speed refrigerated centrifuge (Hunan Hexi Instrument Equipment Co., Ltd.); MILLI- Q ultrapure water system (Millipore, USA).

[0055] 1.2 Sources of reagents and samples

[0056] PS / AL...

Embodiment 260

[0079] Example 2 Identification of 60 batches of Polygonatum medicinal materials

[0080] In order to further confirm the reliability of the experimental results, the number of samples to be tested was increased, and the same method as above was used for testing. The results are as follows:

[0081] Samples to be tested: 20 parts of Yunnan Polygonatum; 20 parts of Polygonatum; 20 parts of Polygonatum multiflora.

[0082] They were numbered and shuffled and crushed, and the experimenters did not know the base sources corresponding to the samples with different numbers.

[0083] Adopt the method described herein to measure each sample, the results are as follows:

[0084] Table 2 PCA analysis results

[0085]

[0086] Table 3 PLS-DA analysis results

[0087]

[0088] Table 4 OPLS-DA analysis results

[0089]

[0090] Table 5 HCA analysis results

[0091]

[0092] On this basis, the comprehensive analysis of the above four analysis results can accurately determin...

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Abstract

The invention relates to an identification method for three polygonatum sibiricum with a legal origin. Collected medicinal materials are extracted by utilizing an ultrasonic method; representation isperformed on chemical compositions by utilizing serial connection of ultra-high performance liquid chromatography and electrostatic field orbitrap high resolution mass spectrum (UPLC-Orbitrap MS) technology after an extract solution is purified by a PS / AL solid phase extraction column; and data are analyzed and processed by utilizing multivariate statistical analysis, wherein the multivariate statistical analysis includes principal component analysis (PCA), partial least squares discrimination analysis (PLS-DA), orthogonal corrected partial least square analysis (OPLS-DA), and cluster analysis(HCA). We found that the chemical components of the medicinal materials of three polygonatum sibiricum with the legal origin are significantly different; the constructed analytical method can be usedto distinguish the three polygonatum sibiricum with the legal origin; and research results provide a basis for in-depth study of the medicinal materials of polygonatum sibiricum and scientific formulation with a quality standard.

Description

technical field [0001] The invention relates to the field of identification of traditional Chinese medicines, in particular to the identification of chemical components of Polygonatum. Background technique [0002] Polygonatum was first recorded in the "Famous Doctors" in the Jin Dynasty. It has the functions of nourishing the kidney and essence, nourishing yin and moistening dryness. It is used to nourish and strengthen the body and treat kidney deficiency, dry cough and spleen and stomach weakness. [1] , is one of the commonly used medicines in Chinese medicine. Modern pharmacological studies have proved that Polygonatum has various pharmacological effects such as enhancing immunity, lowering blood fat, blood sugar, and delaying aging. [2] , is an important resource for both medicine and food, and is known as the "King of Blood and Qi Supplement". [3] . [0003] The 2015 edition of "Chinese Pharmacopoeia" stipulates that Polygonatum is derived from the dried rhizomes of...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N27/62G01N33/15
CPCG01N30/90G01N27/622G01N33/15G01N2030/906
Inventor 杨兴鑫俞捷顾雯董金材李凤娇
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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