Efficient liquid-phase detection method of L-citrulline
A detection method and high-efficiency liquid phase technology, applied in the field of chromatographic columns, can solve problems such as complicated operation steps and long pretreatment time, and achieve the effects of convenient operation, simple processing method, and improved work efficiency
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[0029] The embodiment of the present invention provides a high-performance liquid phase detection method for L-citrulline. The high-performance liquid chromatograph used is RIGOL L-3000, and the chromatographic column is an Agilent amino-bonded silica gel column (5 μm, 4.6*250mm). The injection volume was 20 μl (quantitative loop). Including the following steps:
[0030] 1. Prepare L-citrulline reference substance solution according to the concentration of 0.6mg / mL, 0.8mg / mL, 1.0mg / mL, 1.2mg / mL, 1.4mg / mL;
[0031] 2. The reference substance solution is detected by high performance liquid chromatography, and the chromatographic conditions are:
[0032] Chromatographic column: amino bonded silica gel column;
[0033] Mobile phase: a mixed solution of 0.05mol / L potassium dihydrogen phosphate buffer and acetonitrile; the volume ratio of the phosphate buffer to methanol is 60:35;
[0034] Detection wavelength: 205nm;
[0035] Column temperature: 30℃;
[0036] Mobile phase flow...
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