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Efficient liquid-phase detection method of L-citrulline

A detection method and high-efficiency liquid phase technology, applied in the field of chromatographic columns, can solve problems such as complicated operation steps and long pretreatment time, and achieve the effects of convenient operation, simple processing method, and improved work efficiency

Inactive Publication Date: 2018-11-20
JING JING PHARMA
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problems of complex operation steps and long pretreatment time for detecting L-citrulline in the prior art, the present invention provides a high-performance liquid phase detection method for L-citrulline

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  • Efficient liquid-phase detection method of L-citrulline

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Embodiment 1

[0029] The embodiment of the present invention provides a high-performance liquid phase detection method for L-citrulline. The high-performance liquid chromatograph used is RIGOL L-3000, and the chromatographic column is an Agilent amino-bonded silica gel column (5 μm, 4.6*250mm). The injection volume was 20 μl (quantitative loop). Including the following steps:

[0030] 1. Prepare L-citrulline reference substance solution according to the concentration of 0.6mg / mL, 0.8mg / mL, 1.0mg / mL, 1.2mg / mL, 1.4mg / mL;

[0031] 2. The reference substance solution is detected by high performance liquid chromatography, and the chromatographic conditions are:

[0032] Chromatographic column: amino bonded silica gel column;

[0033] Mobile phase: a mixed solution of 0.05mol / L potassium dihydrogen phosphate buffer and acetonitrile; the volume ratio of the phosphate buffer to methanol is 60:35;

[0034] Detection wavelength: 205nm;

[0035] Column temperature: 30℃;

[0036] Mobile phase flow...

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Abstract

The invention relates to an efficient liquid-phase detecting method of L-citrulline. The method is characterized in that the chromatographic conditions include that an amino bonded silica gel column is used as a chromatographic column; a mixed solution of 0.05mol / L of monopotassium phosphate buffering liquid and acetonitrile is used as a flowing phase, and the volume ratio of the phosphate buffering liquid to acetonitrile is 60: 30-40; the detection wavelength is 200-210nm; the column temperature is 25-35 DEG C; the flowing speed of the flowing phase is 0.7-1.0mL / min. With the adoption of themethod, the content of L-citrulline can be quickly and accurately detected; the error can be reduced; the quality is controlled; in addition, the operation is simple; the detection cost is low; the detection time is short; and the detection efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of chromatographic columns, and in particular relates to a high-efficiency liquid phase detection method for L-citrulline. Background technique [0002] L-citrulline, an alpha amino acid, is produced from ornithine and carbamate in the urea cycle, or as a by-product of arginine catalyzed by nitric oxide synthase (NOS) . First arginine is oxidized to N-hydroxy-arginine, which is then oxidized to citrulline and releases nitric oxide. L-citrulline can relax human blood vessels, can be used to enhance male sexual function, and treat sexual dysfunction, and can maintain healthy lung function, improve mental clarity, and help brain nerve cells store and recall information. Function. [0003] The content of L-citrulline needs to be tested for quality during the production process. There are two kinds of chromogenic method and amino acid analyzer detection method disclosed in the prior art, but the chromogenic m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8818
Inventor 李志强薛海英马腾
Owner JING JING PHARMA
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