A strain of Lactobacillus fermentum with high production of ferulic acid esterase and its application

A technology for fermenting lactobacillus and ferulic acid esterase, which is applied in the direction of lactobacillus, application, bacteria used in food preparation, etc., can solve the problems that the output cannot meet the market demand, unfavorable large-scale production, long fermentation time, etc. Achieve the effects of improving quality and protecting the environment, improving utilization rate, and producing acid quickly

Active Publication Date: 2020-07-28
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently used ferulic acid esterase is mainly obtained from the traditional fermentation of wild fungi. The fermentation time is long, the enzyme activity is low and the cost is high, which is not conducive to large-scale production, and the output cannot meet the current market demand.

Method used

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  • A strain of Lactobacillus fermentum with high production of ferulic acid esterase and its application
  • A strain of Lactobacillus fermentum with high production of ferulic acid esterase and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、17

[0050] Isolation and identification of embodiment 1, 17SD-2 bacterial strain

[0051] 1. Isolation of 17SD-2 strain

[0052] 17SD-2 was isolated from fresh corn silage. The specific separation method is as follows: take 10 g of fresh corn silage, add 90 mL of normal saline, and dilute it to 10 -7 , select the appropriate gradient to spread on the MRS medium (1L MRS medium formula is beef extract 10g, casein peptone 10g, yeast extract 5g, glucose 20g, sodium acetate 5g, diamine hydrogen citrate 2g, Tween80 1mL, K 2 HPO 4 2g, MgSO 4 ·7H 2 O 0.58g, MnSO 4 ·7H 2 (0.25g, agar powder 1.5%, distilled water to 1L, autoclaved) or improved MC medium (1L improved MC medium formula is soybean peptone 5g, beef extract powder 3g, yeast extract powder 3g, Glucose 20g, lactose 20g, calcium carbonate 10g, agar powder 1.5%, distilled water to 1L, autoclaved) or BHI brain heart infusion agar medium (1L BHI brain heart infusion agar medium formula is bovine brain Extract powder 12.5g, be...

Embodiment 2

[0057] Embodiment 2, the preparation of microbial preparation 17SD-2

[0058] 1. The 17SD-2 strain isolated and purified in Example 1 was inoculated into MRS liquid medium, and fermented and cultured in a 37° C. incubator for 72 hours to obtain 17SD-2 fermentation broth.

[0059] 2. Centrifuge the 17SD-2 fermentation broth obtained in step 1 for 2-5min at 10000-12000rpm, collect the supernatant to obtain the supernatant of the 17SD-2 fermentation broth (the concentration of the 17SD-2 bacterial strain is 10 14 -10 18 CFU / L). The supernatant of 17SD-2 fermentation broth was named microbial preparation 17SD-2.

Embodiment 3

[0060] Embodiment 3, the application of microbial preparation 17SD-2 in degrading ethyl ferulate

[0061] 1. The supernatant of 17SD-2 fermentation broth degrades ethyl ferulate to produce a transparent circle

[0062] 1. The MRS medium that does not contain glucose is cooled to 50-55 ° C, then ethyl ferulate is added in the medium in an amount of 0.1% (mass fraction), and the plate is inverted to prepare ethyl ferulate as Plates with sole carbon source.

[0063] 2. Use the well-drilling method to drill holes on the plate with the ethyl ferulic acid obtained in step 1 as the only carbon source, and then take 10 μL of the microbial preparation 17SD-2 prepared in Example 2 and add it to the holes. Placed in the medium for 48-72 hours, and physiological saline was used as a negative control.

[0064] The result is as figure 1 shown. The results showed that the microbial preparation 17SD-2 could degrade ethyl ferulate and produce a clear transparent circle. It shows that the ...

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Abstract

The invention discloses a ferulic lactobacillus with high ferulic esterase production and application thereof derived from fresh corn silage. The lactobacillus fermentum provided by the present invention is Lactocacillus fermentium 17SD‑2. It is proved by experiments that the Lactobacillus fermentum 17SD-2 CGMCC No.15448 of the present invention can secrete ferulic acid esterase, has the function of degrading the dense network structure of plant cell walls, and can be used to improve the cellulose digestibility in high-fiber silage raw materials 1. Improve the palatability of silage raw materials, improve the utilization rate of silage raw materials and improve the growth performance of animals. At the same time, it also has the characteristics of fast acid production, short reproduction cycle, fast growth, non-toxic to humans and animals, and no pollution to the environment. The quality and protection of the environment are of great significance, and it also complies with the social development needs of environmental protection and ecological balance.

Description

technical field [0001] The invention relates to a strain of Lactobacillus fermentum derived from fresh corn silage, which can efficiently secrete ferulic acid esterase, accelerate the construction of an acidic environment for silage, and help degrade the fiber content in forage grass, and can be applied to high-fiber forage grass silage And deep processing. Background technique [0002] With the rapid development of the agricultural economy, farmers' living conditions and rural fuel structure have changed, and crop straw has gradually become agricultural waste, and straw burning has become a symbolic phenomenon in the busy farming seasons of spring and autumn. However, the use of incineration or disposal in the field not only leads to environmental pollution and waste of resources, but also poses fire and traffic accident hazards, and also destroys the ability of soil to resist drought and keep moisture, seriously restricting the sustainable development of agriculture. Ther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/18A23K30/18C12R1/225
CPCA23K30/18C12N1/20C12N9/18C12Y301/01073C12R2001/225C12N1/205A23V2400/143
Inventor 钟瑾骆爱群苏日娜倪奎奎陶勇
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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