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Method for stopping and controlling resistant pollutants in soil from migrating and gathering towards interiors of plant bodies through biomass charcoal, plant planting method and application

A technology of biomass charcoal and pollutants, applied in the field of environmental engineering, can solve problems such as the increase in the probability of human pathogenic bacteria acquiring drug resistance, hidden dangers and threats to vegetable safety and human health, and is beneficial to the metabolism of bacteria and beneficial to bacteria. Effect of colonization and full adsorption

Inactive Publication Date: 2018-11-16
BEIJING GEOENVIRON ENG & TECH
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, livestock and poultry manure often contain a variety of antibiotics. After antibiotics and their derivative compounds are applied to farmland soil along with livestock and poultry manure, a large number of antibiotic-resistant indigenous bacteria will be gradually induced and screened out, and these resistance The overexpression and horizontal / vertical diffusion and transfer of resistance genes in the bacteria also greatly increase the probability of human pathogenic bacteria acquiring drug resistance in the soil, resulting in new characteristics of compound pollution of antibiotics, resistant bacteria and resistant genes in the soil, while Previously, scientific and technological personnel paid little attention to this new type of contaminated soil and lacked awareness; in addition, when humans plant vegetables on such high-abundance resistance gene-contaminated farmland soil, some indigenous resistant bacteria will accompany the growth of vegetables Entering the vegetable tissue to form resistant endophytic bacteria, at the same time the resistance gene also migrates into the plant, and then brings extremely serious hidden dangers and threats to vegetable safety and human health in the food chain. Much attention has been paid to the detection of pollutants such as heavy metals, pesticides and antibiotics in vegetables, but the process of indigenous resistant bacteria migrating to vegetables to colonize and become endogenous resistant bacteria has been ignored
Therefore, there has not been a high-efficiency control technology for the process of continuously accumulating antibiotics, resistant bacteria and resistant genes in crops so far. It is very important to apply the resistance control method of various resistant pollutants to prevent the migration and enrichment of various antibiotics, resistant bacteria and resistant genes in the soil to plants.

Method used

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  • Method for stopping and controlling resistant pollutants in soil from migrating and gathering towards interiors of plant bodies through biomass charcoal, plant planting method and application
  • Method for stopping and controlling resistant pollutants in soil from migrating and gathering towards interiors of plant bodies through biomass charcoal, plant planting method and application
  • Method for stopping and controlling resistant pollutants in soil from migrating and gathering towards interiors of plant bodies through biomass charcoal, plant planting method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Technical example of biochar blocking and controlling the migration and enrichment of tetracycline, sulfadiazine and their corresponding resistant bacteria in radish

[0084] Target soil: Farmland soil near the Hengliang Dairy Farm in Pukou, Nanjing, which has been polluted by cow manure containing high concentrations of antibiotics for a long time. The average background concentration of tetracycline in soil is 12.1±1.3mg kg -1 , the average background contamination of sulfadiazine was 15.2±2.3mg kg -1 , the relative abundances of tetracycline resistance genes tetM, tetW and tetQ per gram of fresh weight soil were 2.4×10 -3 , 3.2×10 -4 and 4.2×10 -4 Tet copy number / 16S copy number, relative abundance of sulfonamide resistance genes sulI and sulII per gram fresh weight of soil were 1.6×10 -3 and 3.6×10 -4 tet copy number / 16S copy number.

[0085] First, select biochar prepared from corn stalks, crush it mechanically, and sieve the biochar with a sterile mesh sieve...

Embodiment 2

[0088] Technical example of biochar blocking and controlling the transfer and enrichment of soil roxithromycin, ofloxacin and their corresponding resistant bacteria and resistance genes to lettuce

[0089] Target soil: Farmland soil near a chicken farm in the Hushu area of ​​Nanjing City that has been polluted by livestock and poultry manure containing high concentrations of antibiotics for a long time. The average background contamination concentration of roxithromycin in the soil was 8.3±0.6mg kg -1 , the average background contamination of sulfadiazine was 9.3±0.5mg kg -1 , the relative abundances of roxithromycin resistance genes ermA and ermB per gram of fresh weight of soil were 9.1×10 -3 and 5.5×10 -4 erm copy number / 16S copy number, the relative abundance of ofloxacin-like resistance genes qnrB and qnrS1 in each gram of fresh weight soil are 2.3×10 -3 and 5.9×10 -4 qnr copy number / 16S copy number.

[0090] First, select biochar prepared from wheat straw, crush it ...

Embodiment 3

[0093] Technical example of biochar blocking and controlling soil penicillin, chloramphenicol and their corresponding resistant bacteria and resistance gene migration and enrichment to pepper fruit

[0094] Target soil: farmland soil near a pig farm in Guli District, Nanjing City, which has been polluted by pig feces containing high concentrations of antibiotics for a long time. The average background concentration of penicillin in soil is 6.2±0.5mg kg -1 , the average background contamination of chloramphenicol was 4.7±0.3mg kg -1 , the relative abundance of the penicillin resistance gene ampC per gram of fresh weight soil is 4.5×10 -5 amp copy number / 16S copy number, the relative abundance of chloramphenicol resistance genes catI and catII per gram of fresh weight soil is 6.7×10 -5 and 3.8×10 -5 cat copy number / 16S copy number.

[0095] Select the biochar prepared from cotton stalks, crush it mechanically, and sieve the biochar with a 10-mesh sterile mesh sieve, and then...

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Abstract

The invention provides a method for stopping and controlling resistant pollutants in soil from migrating and gathering towards the interiors of plant bodies through biomass charcoal, a plant plantingmethod and application and relates to the technical field of environmental engineering. According to the method, the biomass charcoal is mixed with soil, and then the field moisture capacity of the soil is adjusted, kept for 5-20 days and preferentially kept for 7-15 days; the resistant pollutants comprise antibiotics, resistant thalli and resistant genes. According to the method, the technical problem is solved that in the prior art, a method for effectively stopping and controlling the resistant pollutants in the soil from migrating and gathering towards the interiors of the plant bodies does not exist; the method for stopping and controlling the resistant pollutants from migrating and gathering is suitable for the soil polluted by various antibiotics, resistant thalli and resistant genes and has a good stopping and controlling effect. In addition, the invention further provides the corresponding plant planting method, and the method for stopping and controlling the resistant pollutants in the soil from migrating and gathering towards the interiors of the plant bodies through the biomass charcoal and the plant planting method can be applied in the fields of agriculture and forestry.

Description

technical field [0001] The invention relates to the technical field of environmental engineering, in particular to a method for preventing and controlling the migration and enrichment of resistant pollutants in soil to plants by biomass charcoal, a plant planting method and its application. Background technique [0002] In my country, livestock and poultry manure are often directly applied to farmland soil as organic materials without safe treatment. However, livestock and poultry manure often contain a variety of antibiotics. After antibiotics and their derivative compounds are applied to farmland soil along with livestock and poultry manure, a large number of antibiotic-resistant indigenous bacteria will be gradually induced and screened out, and these resistance The overexpression and horizontal / vertical diffusion and transfer of resistance genes in the bacteria also greatly increase the probability of human pathogenic bacteria acquiring drug resistance in the soil, resul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/00A01G22/05A01G22/15A01G22/25A01G24/10A01B79/02
CPCA01B79/02A01G22/00A01G22/05A01G22/15A01G22/25A01G24/10
Inventor 孙大伟叶茂吕正勇朱湖地范吉强宋登慧
Owner BEIJING GEOENVIRON ENG & TECH
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