Detection system for detecting CYP2D6*10 gene mutation and kit of detection system

Abstract

The invention relates to a detection system for detecting CYP2D6*10 gene mutation and a kit of the detection system. The detection system comprises digital PCR (polymerase chain reaction) premixed solution and primer probe mixed solution, the primer probe mixed solution comprises an upstream primer, a downstream primer and a peptide nucleic acid probe which are used for detecting CYP2D6*10 sites of CYP2D6 genes, a nucleotide sequence of the upstream primer is as shown in SEQ ID No.1, a nucleotide sequence of the downstream primer is as shown in SEQ ID No.2, a nucleotide sequence of the peptidenucleic acid probe and CYP2D6*10 mutant gene sites are completely complemented, the nucleotide sequence of the peptide nucleic acid probe is as shown in SEQ ID No.3, and the 5' end of the peptide nucleic acid probe is connected with a strong chelation group formed by four amino acids. According to the detection system for detecting CYP2D6*10 gene mutation and the kit of the detection system, sensitivity and specificity are high, needed samples are less, and quantitative PCR detection can be performed rapidly, conveniently and accurately.

Description

technical field [0001] The invention relates to a detection product for detecting CYP2D6*10 gene mutation, which belongs to the field of biotechnology. Background technique [0002] Isoquine 4'-hydroxylase (CYP2D6) is an important member of the second subfamily of the cytochrome P450 superfamily (cytochrome P450s, CYP). CYP2D6*10 genotype polymorphism is closely related to the survival rate of breast cancer patients treated with tamoxifen (TAM). The activity of CYP2D6 in the population presents a four-state distribution phenomenon of intensive metabolizers (EM), intermediate metabolizers (IM), poor metabolizers (PM) and ultra metabolizers (UM). At present, more than 100 mutant genes of CYP2D6 have been found, and its mutation type determines the metabolic activity of the enzyme. CYP2D6 gene polymorphism determines the difference in drug metabolism intensity, and affects the selection of clinical drug varieties and doses. In addition to participating in the metabolism of s...

AI Technical Summary

Problems solved by technology

At present, technologies such as next-generation sequencing, FLP, allele-specific oligonucleotide hybridization, allele-specific PCR, and gene chips are mainly used, but the operation process is cumbersome, and reaction tubes need to be replaced, which is prone to pollution. post-processing, affecting the accuracy of the test results

Or the instruments and equipment are expensive, and it is difficult to promote and apply them on a large scale

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