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Probe, and preparation method and application thereof

A technology of probes and reagents, which is applied in the field of multi-target ion detection probes and its preparation and application, and can solve problems such as single function, unreported fluorescent probes, and limited application range

Active Publication Date: 2018-11-09
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the structure of various fluorescent probes, it has various limitations such as single function and limited application range in analytical applications.
At present, most fluorescent probes can only be used for the detection of metal ions or acid anions, and no fluorescent probes that can be used to detect specific metal ions and water at the same time have been reported.

Method used

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  • Probe, and preparation method and application thereof
  • Probe, and preparation method and application thereof
  • Probe, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] 1. A probe whose chemical structural formula is:

[0101]

[0102] The synthesis route of the probe is as follows:

[0103]

[0104] The specific preparation method of the probe is as follows:

[0105] Under nitrogen protection, take tris(2-aminoethyl)amine (4.00g, 27.36mmol) in a 100ml three-necked flask, measure 20mL of absolute ethanol, stir and heat up to reflux, then weigh rhodamine B (1.64g, 3.42mmol) was dissolved in 40mL of absolute ethanol, added to a constant pressure funnel, slowly added dropwise into a three-necked flask, refluxed for 36h after the dropwise addition was completed, the ethanol was evaporated under reduced pressure, extracted with dichloromethane (3×100mL), and the organic phase Dry with anhydrous sodium sulfate overnight, distill off the solvent to obtain a red viscous substance, which is separated by silica gel column chromatography, and the eluent is methanol / chloroform / triethylamine=9 / 1 / 1(v / v / v ), to obtain 1.63g of colorless visco...

Embodiment 2

[0107] Embodiment 2. Reagent preparation

[0108] (1) Preparation of the probe solution: Weigh 10.76 mg of the probe prepared in Example 1, dissolve it with 1.4 dioxane, and prepare 10 mL of the probe stock solution with a concentration of 1 mM;

[0109] (2)Hg 2+ Preparation of stock solution: Weigh 226.8 mg of mercury perchlorate, dissolve it in ultrapure water, and prepare 50 mL of an aqueous solution with a concentration of 20 mM; another weigh mercuric perchlorate, dissolve it with acetonitrile, and prepare an anhydrous solution with a concentration of 1 mM.

[0110] (3)Al 3+ Preparation of stock solution: Weigh 343.3mg of aluminum perchlorate nonahydrate, dissolve it in ultrapure water, and prepare 50mL of an aqueous solution with a concentration of 20mM; aqueous solution;

[0111] (4) Other metal ions (Li + , Na + , K + , Mg 2+ , Ca 2+ , Ba 2+ ,Sr 2+ , Fe 3+ ,Sr 2+ , Zn 2+ ,Co 2+ , Ni 2+ , Cd 2+ , Pb 2+ , Cr 3+ , Ag + , Cu 2+ , Mn 2+ ) preparation of...

Embodiment 3

[0112] Embodiment 3. Fluorescence spectrometry detects Hg 2+ 、Al 3+

[0113] 1. Detection of Hg 2+

[0114] Add the probe solution (1mM, 100μL) into a 10mL volumetric flask and dilute it with 1,4-dioxane / water so that the composition of the probe solution is 97% by volume of 1,4-dioxane / water / 3, shake well. Add 3ml into a 1cm cuvette, and use 360nm as the fluorescence excitation wavelength to measure the fluorescence spectrum.

[0115] The solution with a volume ratio of 1,4-dioxane / water of 97 / 3 and a probe concentration of 10 μM has almost no fluorescence emission. Add 100 μM metal ion: Hg 2+ , Al 3+ , Li + , Na + , K + , Zn 2+ , Mg 2+ , Ca 2+ , Ba 2+ ,Sr 2+ , Cr 3+ , Fe 3+ ,Co 2+ , Ni 2+ , Cu 2+ , Pb 2+ , Cd 2+ , Ag + , Mn 2+ , placed for 1 hour, only Al 3+ , Hg 2+ The addition of the probe significantly enhanced the fluorescence peak at 585nm (see figure 1 ).

[0116] In the probe solution with a volume ratio of 1,4-dioxane / water of 97 / 3 and a ...

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Abstract

The invention discloses a probe, and a preparation method and an application thereof. The chemical name of the probe is tris[2,2'-bis[(4-benzothiazole-2-yl)-2,5-dihydroxybenzaldehyde]aminoethyl-2-"-rhodamineformylaminoethyl]amine; and the probe is mainly prepared from tris(2-aminoethyl)amine, rhodamine B and benzothiazolecarboxaldehyde. The probe can realize single-probe multi-target detection, that is, the probe can be used for simultaneously detecting Hg<2+>, Al<3+> and / or H2O, and the probe has the advantages of low detection cost and high detection efficiency. The probe facilitates the analysis of a complex microscopic system.

Description

technical field [0001] The invention relates to a probe and its preparation and application, in particular to a multi-target ion detection probe and its preparation and application. Background technique [0002] Fluorescent probes are widely used in the fields of chemistry, biology and environmental science as an important analytical detection technology due to their simplicity, high sensitivity, real-time and visual detection. There have been many reports on fluorescent probes used to detect important metal ions and anions related to life and the environment. [0003] Hg 2+ As a toxic heavy metal ion that is most concerned by the environment, the research on its existence and ultra-low content detection method is of great significance to environmental protection and biological safety. As one of the widely used metallic materials, Al 3+ Research on rapid and sensitive analytical methods for detection and quality control in the environment, food, drug additives and packagi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/107C09K11/06G01N21/64G01N21/31
CPCC07D491/107C09K11/06C09K2211/1007C09K2211/1029C09K2211/1037C09K2211/1088G01N21/31G01N21/64G01N21/6428G01N21/6447
Inventor 曾晞田雁许万里廖贤方浚安牟兰
Owner GUIZHOU UNIV
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