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Immunochromatographic test strip capable of simultaneously detecting sulfaquinoxaline and norfloxacin and preparation method thereof

An immunochromatographic test strip and sulfaquinoxaline technology, which is applied in the field of immunochromatographic test strips, can solve the problems of shortening the detection time of kinetic food, inaccurate detection, and low detection sensitivity, and achieve intuitive detection results. Good sensitivity and low detection cost

Inactive Publication Date: 2018-11-06
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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Problems solved by technology

[0004] Most of the common immunochromatographic test strips detect one substance, but few detect two substances at the same time. The only multi-residue detection also has problems such as low detection sensitivity and inaccurate detection; simultaneous detection of sulfaquinoxaline and The immunochromatographic test strips for the two veterinary drugs of floxacin have not been reported so far. The development of this kind of test strips greatly shortens the time of testing kinetic food and improves the detection efficiency

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  • Immunochromatographic test strip capable of simultaneously detecting sulfaquinoxaline and norfloxacin and preparation method thereof

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Embodiment 1

[0023] A preparation method for simultaneous detection of sulfaquinoxaline and norfloxacin multi-residue immunochromatographic paper strips

[0024] 1. Preparation of colloidal carbon:

[0025] Disperse the carbon black into the borate buffer solution and sonicate for 20 min to obtain a uniformly dispersed colloidal solution with a concentration of 0.5 mg / mL.

[0026] 2. Preparation of antibody-label conjugates

[0027] Preparation of Sulfaquinoxaline Antibody—Colloidal Carbon Label:

[0028] Dilute the norfloxacin antibody to 0.5mg / mL with borate buffer solution; add the antibody to the colloidal carbon solution under slow stirring conditions, and then slowly stir overnight in a refrigerator at 4°C; use the buffer solution at 14000r / min, Wash the carbon-labeled antibody for 3 times at 10°C for 30 minutes, and finally resuspend the precipitate with buffer to the original volume, and control the concentration of the antibody between 250 μg / mL and 100 μg / mL, and store it at 4°...

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Abstract

The invention provides an immunochromatographic test strip capable of simultaneously detecting residues of two veterinary drugs such as sulfaquinoxaline and norfloxacin. The immunochromatographic teststrip comprises a sample pad, a conjugate pad, a nitrocellulose membrane, an absorbent pad and a PVC (Polyvinyl Chloride) backing plate, and is characterized in that the sample pad, the conjugate pad, the nitrocellulose membrane and the absorbent pad are sequentially adhered onto the PVC backing plate; one half of the conjugate pad covers the nitrocellulose membrane; the sample pad partially covers the conjugate pad; the absorbent pad partially covers the nitrocellulose membrane; a quality control line and two detection lines are arranged in parallel on the nitrocellulose membrane. The invention further discloses a preparation method of the test strip based on two visual colors. The immunochromatographic test strip disclosed by the invention has the outstanding advantages that the immunochromatographic test strip capable of simultaneously detecting the residues of the veterinary drugs such as sulfaquinoxaline and norfloxacin in the invention is realized by utilizing a specific antigen-antibody reaction. Therefore, the test strip is high in specificity and excellent in sensitivity.

Description

technical field [0001] The invention relates to an immunochromatographic test strip method for rapidly detecting multiple residues of two veterinary drugs, sulfaquinoxaline and norfloxacin, in animal-derived foods, in particular, two visual color markers are simultaneously used on the same test strip. The multi-residue immunochromatographic test strip for detecting sulfaquinoxaline and norfloxacin veterinary drugs and its preparation method. Background technique [0002] Sulfonamides were the first synthetic antibiotics. P-aminobenzenesulfonamide is the classic basic structure of sulfanilamide drugs, and its modification shows good activity. The derivatives also belong to sulfanilamide drugs. Different functional groups for R and R' are different new sulfa drugs. Sulfa drugs can inhibit the synthesis of folic acid, thereby inhibiting the synthesis of nucleic acid, and finally achieve the purpose of inhibiting bacterial reproduction. The wide application and long cycle of s...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/558G01N33/577
Inventor 刘冰孙晓张燕生威王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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