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Preparation method for fugu rubripes uronema marinum disease vaccine

A technology for red fin puffer and cercariasis, which is applied in the field of vaccine preparation, can solve the problem that there is no preparation of red fin fugu marine cercariasis vaccine, the inability to completely kill marine cercariasis, and the ineffectiveness of Chinese herbal medicine treatment methods To prevent parasites and infections, improve immunity, and reduce economic losses

Active Publication Date: 2018-11-06
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physiotherapy methods such as fresh water baths cannot completely kill marine C.
So far, there is no report on the preparation of a vaccine against marine filariasis in redfin puffer

Method used

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  • Preparation method for fugu rubripes uronema marinum disease vaccine
  • Preparation method for fugu rubripes uronema marinum disease vaccine
  • Preparation method for fugu rubripes uronema marinum disease vaccine

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Experimental program
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Embodiment

[0018] a. The worm body was isolated from the festered tissue of the redfin fugu body surface in a farm, and was identified as the larvae of the marine filariasis ( Uronema marinum ). Inoculate the isolated parasites into broth medium, and carry out in vitro acclimatization and cultivation for 15 days to obtain the liquid of marine cercariasis in vitro acclimatization culture. The broth medium is boiled with 5 g of homogenized fish meat per 100 ml of sterilized seawater 10min, then filter the filtrate with 200 mesh gauze;

[0019] b. Inoculate the cultured marine C. filariae in vitro into the broth medium, the volume ratio of the inoculum to the broth medium is 1:100, and cultivate at 20°C for 120 hours to obtain C. marine C. liquid;

[0020] c. Draw 1ml of marine C. filariae liquid in the growth phase into a sterilized 1.5ml centrifuge tube, centrifuge at 2000rpm for 10 minutes, discard the supernatant and add 1ml L-15 complete medium to the centrifuge tube, the L- The 15...

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Abstract

The invention discloses a preparation method for a fugu rubripes uronema marinum disease vaccine. The preparation method comprises the following steps that: picking up a single uronema marinum to be inoculated to an L-15 complete medium to be subjected to pure culture; then, carrying out enlargement cultivation; adding 100 mu l / L of formalin to kill larvae; carrying out centrifugation for 10 min at 4000rpm by a low-speed centrifugal machine to collect sediments; adding into the L-15 complete medium to prepare inactivation larva liquid of which the density is 2 million / ml; collecting in a centrifuge tube of 1.5ml, and preserving at the temperature of -80 DEG C. Before immunization is carried out, dead larvae are unfrozen on ice, are broken by electromagnetic wave and are mixed with equivalent Freund's adjuvant or Freund's incomplete adjuvant, and the mixed liquid is oscillated to emulsion to prepare the fugu rubripes uronema marinum disease vaccine. Since an intraperitoneal injection way is adopted, the immunity of the fugu rubripes for the uronema marinum disease can be improved.

Description

technical field [0001] The invention relates to a preparation method of a vaccine, in particular to a preparation method of a vaccine for preventing marine filariasis of redfin puffer. Background technique [0002] The marine filariasis belongs to the shield ciliates, which is a small facultative parasitic ciliate. It parasitizes the red-finned puffer and can cause the marine filariasis of the red-finned puffer. The disease is highly contagious and has a wide range of transmission. It can cause increased mucus on the body surface, blackened body color, white turbidity, ulceration or peeling of the back, redness and swelling of the head, swelling, congestion, water accumulation inside the gill cover, discoloration or erosion of the gills, and abdominal pain. Swelling, local swelling, hemorrhage or ulceration, symptoms such as fin hemorrhage and ulceration cause a large amount of economic losses to the redfin pufferfish aquaculture industry. Therefore, the prevention of marin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/002A61K39/39A61P33/02A61P37/04
CPCA61K39/002A61K39/39A61K2039/521A61K2039/552A61P33/02A61P37/04
Inventor 黎睿君高延奇叶仕根张涛
Owner DALIAN OCEAN UNIV
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