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Quick seedling cultivating method for red honeysuckle stems

A technology of honeysuckle and stem segments, which is applied in the field of rapid seedling growth of red honeysuckle stem segments, which can solve the problems of plant carrying, poor growth and development, and low plant survival rate, and achieve good growth, fast survival, and high survival rate.

Active Publication Date: 2018-11-06
SHANGQIU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many studies have been carried out on the tissue culture plants of honeysuckle, but there are relatively few research reports on the tissue culture rapid propagation technology of red honeysuckle
Moreover, the existing red honeysuckle is mainly propagated by cuttings, but there are problems that the plants carry viruses, miscellaneous bacteria, poor growth and development, and the survival rate of the plants is not high.

Method used

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  • Quick seedling cultivating method for red honeysuckle stems
  • Quick seedling cultivating method for red honeysuckle stems
  • Quick seedling cultivating method for red honeysuckle stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of rapid seedling-forming method of red honeysuckle stem section, comprises the following steps:

[0022] S1, collect 2-year-old young shoots of red honeysuckle with internodes, remove leaves, soak in 2% washing powder solution for 10 minutes to remove surface dirt, then rinse with tap water for 1 hour and soak for 30 minutes, then rinse repeatedly with sterile water for 3- 5 times, in a sterile environment, soak in ethanol with a volume concentration of 75% for 15 seconds, then rinse with sterile water, then put it into a mercuric chloride solution with a mass concentration of 0.2%, stir for 3 minutes, take it out and rinse with sterile water 5-6 times, and finally use sterile filter paper to absorb the moisture on the surface of the branches to obtain sterile branches;

[0023] S2, add sucrose and agar to MS medium, and obtain basal medium after high temperature and high pressure sterilization; add 6-BA (6-benzylaminopurine), NAA (naphthaleneacetic acid), KT (k...

Embodiment 2

[0027] A kind of rapid seedling-forming method of red honeysuckle stem section, comprises the following steps:

[0028] S1, collect 2-year-old young shoots of red honeysuckle with internodes, remove leaves, soak in 2% washing powder solution for 13 minutes to remove surface dirt, then rinse with tap water for 1 hour and soak for 35 minutes, then rinse repeatedly with sterile water for 3- 5 times, soak in ethanol with a volume concentration of 75% for 20s in a sterile environment, then rinse with sterile water, then put it into a mercuric chloride solution with a mass concentration of 0.2%, stir for 3 minutes, take it out and rinse with sterile water 5-6 times, and finally use sterile filter paper to absorb the moisture on the surface of the branches to obtain sterile branches;

[0029] S2, add sucrose and agar to MS medium, and obtain basal medium after high temperature and high pressure sterilization; add 6-BA (6-benzylaminopurine), NAA (naphthaleneacetic acid), KT (kinetin ...

Embodiment 3

[0033] Discussion on the optimal addition amount of different hormones (6-BA, NAA, KT) in the induction medium:

[0034] Test material: the aseptic branch of band axillary bud in the S1 of embodiment 1 is cut into 1cm stem section;

[0035] Test method: the final concentration of 6-BA is 1.0mg / L, 1.5mg / L, the final concentration of NAA is 0.1mg / L, 0.2mg / L, and the final concentration of KT is 0.5mg / L, 1.0mg / L, 1.5 mg / L, 12 kinds of induction medium were made by orthogonal combination. Inoculate test materials in 12 kinds of induction media respectively, calculate germination rate and contamination rate, wherein contamination rate=contamination number / inoculation number×100%, germination rate=germination number / (total number of buds-pollution bud number)×100% , the induction medium preparation method and the culture method are all consistent with Example 1. The results are shown in Table 1:

[0036]Table 1 Effects of induction media with different concentrations and ratios on...

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Abstract

The invention belongs to the technical field of agricultural cultivation and particularly relates to a quick seedling cultivating method for red honeysuckle stems. The quick seedling cultivating method comprises the steps that red honeysuckle tender branches among stems are collected, are subjected to immersion cleaning with washing powder, are flushed with sterile water, are soaked with ethyl alcohol and mercuric chloride and then are flushed with sterile water; NAA, KT and 6-BA are added into an MS culture medium to prepare an inducing culture medium, a growth culture medium and a proliferation culture medium; the branches subjected to sterile treatment are cut into stem sections to be inoculated to the three culture mediums in sequence, and red honeysuckle seedlings are obtained. The red honeysuckle stems with axillary buds are selected as a breeding material and are sequentially inoculated in the culture mediums added with the NAA, KT and 6-BA which are different in concentration,and the red honeysuckle seedlings free of virus infection, growing well, quick to survive and high in survival rate are cultivated.

Description

technical field [0001] The invention belongs to the technical field of agricultural cultivation, in particular to a method for rapid seedling growth of red honeysuckle stem segments. Background technique [0002] Red honeysuckle (Lonicerajaponica var. chinensis) is a semi-evergreen shrub of Lonicera japonica and Lonicera genus, and is one of the varieties of Lonicera japonica Thund. The color of red honeysuckle flower buds is full of changes. Before opening, it changes from purple red to bright red. When the flowers bloom, they are pink at first, and then red, yellow and white. It is pleasing to the eye and very beautiful. Purple-red, with drooping vines, elegant and unique posture, and very high ornamental value; the fragrance of the flowers is overflowing, stronger than that of ordinary honeysuckle, and its flowers, leaves, and stems can be used as medicine. It is a unique variety in honeysuckle . [0003] Plant tissue culture technology refers to the cultivation of isol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 马丽郭学良韩霜李亚静肖志勇
Owner SHANGQIU NORMAL UNIVERSITY
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