Methods for obtaining and correcting biological sequence information

A technology of sequence information and sequencing, which is applied in the fields of bioinformatics, biochemical equipment and methods, and the determination/inspection of microorganisms, and can solve problems such as no sequencing logic innovation.

Pending Publication Date: 2018-10-23
CYGNUS BIOSCI BEIJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the optimization of accuracy in existing technologies mostly focuses on optimizing the chemical reaction itself and subsequent image signal processing, without innovation in sequencing logic

Method used

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  • Methods for obtaining and correcting biological sequence information
  • Methods for obtaining and correcting biological sequence information
  • Methods for obtaining and correcting biological sequence information

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0376] Example 1: Sequencing by "2+2 monochrome" method

[0377] In order to further describe the present disclosure, specific examples are provided below. Unless otherwise indicated, specific parameters, procedures, etc. are conventional in the art. The specific examples are not intended to limit the scope of the invention.

[0378] For sequencing by the "2+2 monochrome" method, three sets of reactions were prepared. Each set includes two vials, each containing two bases labeled with the same fluorophore X. For each set, two vials contained exactly all four bases of the sequencing reaction. 6 bottles (two bottles in each set) solutions do not repeat each other.

[0379] Table 7: Reaction solution in the "2+2 monochrome" method

[0380]

first bottle

second bottle

The first set

AX+CX

GX+TX

second set

AX+GX

CX+TX

third set

AX+TX

CX+GX

[0381] A complete sequencing process includes three rounds of sequenc...

Embodiment 2

[0449] Embodiment 2: Sequencing by "2+2 two-color" method

[0450] In this example, three sets of reaction solutions were prepared. There are two vials per set, each containing two nucleotide bases. The 2 nucleotide bases in each vial are labeled with two different fluorophores (so that they emit at different wavelengths) to distinguish the signals from the two nucleotide bases.

[0451] In this example, the two types of fluorophores are X and Y. For each set, two vials contained exactly all four bases of the sequencing reaction. 6 bottles (two bottles in each set) solutions are not repeated.

[0452] Table 8: Reaction solution in the "2+2 two-color" method

[0453]

first bottle

second bottle

The first set

AX+CY

GX+TY

second set

AX+GY

CX+TY

third set

AX+TY

CX+GY

[0454] A complete sequencing process includes three rounds of sequencing performed sequentially in any suitable order. Each round of sequenci...

Embodiment 3

[0464] Embodiment 3: comparative example

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Abstract

A method for sequencing a biological molecule, such as a nucleic acid molecule, and a method for detecting and / or correcting sequencing error(s) in the sequencing results are provided. Kits and systems based on the above methods are also provided.

Description

technical field [0001] The present invention relates to a high-throughput sequencing method in some aspects, and belongs to the field of gene sequencing. Background technique [0002] High-throughput sequencing is a technology that has developed rapidly in recent years. Compared with traditional Sanger sequencing (Sangersequencing), the biggest advantage of high-throughput sequencing is that it can read massive sequence information at the same time. Although the accuracy is not as good as traditional sequencing methods, due to massive data analysis, information beyond the sequence itself can be obtained, such as gene expression and copy number changes. [0003] Today's mainstream sequencers all use the SBS (sequencing by synthesis) method, such as Solexa / Illumina, 454, IonTorrent, etc. The structure of these sequencers is similar, including fluidic system, optical system and chip system. The sequencing reaction takes place within the chip. The sequencing process is also ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869G06F19/20G16B30/00G16B30/10
CPCC12Q1/6869G16B20/30G16B40/10C12Q2535/122G16B30/00G16B30/10C12Q2537/165G06F17/10
Inventor 黄岩谊陈子天周文雄段海峰康力乔朔
Owner CYGNUS BIOSCI BEIJING CO LTD
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