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Method for detecting new psychoactive substance MeOPP

A detection method and mass spectrometry technology, which is applied in the detection field of the new psychoactive substance MeOPP, can solve the problems of complex biological sample matrix, troublesome detection work, difficult purification, etc., and achieve favorable analysis operation, high-efficiency qualitative and quantitative analysis, and low cost Effect

Inactive Publication Date: 2018-10-23
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing analysis methods focus on qualitative analysis
At the same time, in the actual investigation process, most of the detection objects required are biological samples, such as blood, urine, etc. Biological samples have the characteristics of complex matrix, many interferences, and difficult purification, which brings great trouble to the actual detection work.

Method used

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  • Method for detecting new psychoactive substance MeOPP
  • Method for detecting new psychoactive substance MeOPP
  • Method for detecting new psychoactive substance MeOPP

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1, GC-MS detection of MeOPP in urine samples

[0075] (1) Preparation of sample solution

[0076] An Oasis HLB solid phase extraction cartridge (60 mg, 3 mL) was first pretreated with 2 mL of methanol, 2 mL of deionized water and 2 mL of ammonium acetate (10 mM, pH=9.5). After sample loading, the solid-phase extraction cartridge was washed with 1 mL of deionized water, and then dried in vacuum. Elute with 1 mL of methanol, blow to dryness with nitrogen, redissolve with methanol to 400 μL, filter and centrifuge, and put into a sample vial.

[0077] (2) Setting of detection conditions

[0078] The setting process of gas chromatography conditions is as follows:

[0079] The carrier gas is helium;

[0080] Chromatographic column is DB-5MS or HP-5MS;

[0081] The heating program is as follows: the initial temperature is 70°C, rising to 300°C at a rate of 10°C / min, and keeping for 7 minutes;

[0082] Mass spectrometry conditions: the voltage scanning range is...

Embodiment 2

[0086] Embodiment 2, gas chromatography detects MeOPP in the hair

[0087] (1) Preparation of sample solution

[0088] Basically the same as in Example 1.

[0089] (2) Preparation of standard working solution

[0090] Take the MeOPP standard stock solution and dilute it sequentially into a series of standard solutions with mass concentrations of 1.0, 0.5, 0.25, 0.125, 0.1, 0.05, 0.01, 0.005, and 0.001 mg / mL.

[0091] Take 1 μL of standard solutions of different concentrations and inject them, and record the corresponding peak area.

[0092] Each concentration was injected 3 times, and linear regression was performed on the average A of the 3 peak areas and the mass concentration c (mg / mL) to obtain the regression equation of MeOPP: A=614.77c+0.4875, r 2 It is 0.9996, the linear range is 5~1000μg / mL, and the detection limit is 5μg / mL (S / N≥3).

[0093] (3) Setting of detection conditions

[0094] The carrier gas is nitrogen;

[0095] Chromatographic column is DB-5 or HP-5;...

Embodiment 3

[0107] Embodiment 3, liquid chromatographic detection of MeOPP in blood

[0108] (1) Preparation of sample solution

[0109] Basically the same as in Example 1.

[0110] (2) Preparation of standard working solution

[0111] Take the standard stock solution of MeOPP and dilute it sequentially into a series of standard solutions with mass concentrations of 1.0, 0.75, 0.5, 0.1, 0.05, 0.025, 0.01, 0.005, 0.001, and 0.0005 mg / mL.

[0112] Take 10 μL of standard solutions of different concentrations and inject them, and record the corresponding peak area.

[0113] Each concentration is injected 3 times, and linear regression is performed on the average value A of the peak area of ​​the 3 times and the mass concentration c (mg / mL), and the regression equation of MeOPP is A=50521c-283213, r 2 =0.9992, the linear range is 5-1000 μg / mL, the limit of quantification is 5 μg / mL (S / N≥3).

[0114] (3) Setting of detection conditions

[0115] The column is 00SB-C18 (4.6×150mm, 5μm);

...

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Abstract

The invention discloses a method for detecting new psychoactive substance MeOPP. The precise and efficient method for determining the MeOPP in biological samples includes qualitative and quantitativeanalysis methods such as gas chromatography-mass spectrometry, gas chromatography, liquid chromatography and liquid chromatography-mass spectrometry; the biological samples involved may be at least one of blood, urine, saliva and hairs. Due to the large number of interfering factors in the biological samples, the pretreatment process of the samples is very important. The MeOPP in the biological samples can be effectively extracted by the solid phase extraction process according to a large number of experiments, and high-efficiency qualitative and quantitative analysis is realized. Each analytical method has high sensitivity and a wide linear range. The cost of a detector is low, and the method facilitates automated analytical operation.

Description

technical field [0001] The invention relates to a detection method of MeOPP, a new psychoactive substance, and belongs to the field of criminal investigation drug detection. Background technique [0002] MeOPP (1-4-methoxyphenylpiperazine) belongs to the piperazine class of new psychoactive substances with the molecular formula C 11 h 16 N 2 O has a molecular weight of 192.26 g / mol, and its structural formula is shown in formula (1). It has a stimulating effect and is a non-selective 5-HT receptor antagonist. It is often sold mixed with piperazine derivatives to enhance the effect. [0003] By consulting a large number of domestic and foreign literatures, the reported analysis methods of MeOPP include color reaction, microcrystal experiment, thin layer chromatography, gas chromatography, high performance liquid chromatography, capillary electrophoresis and Fourier transform infrared, etc. However, the existing analytical methods mostly focus on qualitative analysis. At...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 常颖赵阳高利生郑珲张春水翟晚枫李彭赵彦彪郑晓雨杨虹贤刘克林钱振华
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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