Method for detecting sensitization of cosmetics components by ultra-weak biophoton imaging system

A technology of biophotonics and imaging systems, which is applied in the direction of material analysis, test sample preparation, and measuring devices through optical means, which can solve the problems of time-consuming, high cost, and large individual differences among volunteers, and achieve simple and fast operation Effect

Active Publication Date: 2018-10-23
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

However, the detection process of the human patch test is time-consuming and requires a large number of volunteers to participate, and there are large individual differences among volunteers.
However, the currently popular in vitro alternative animal experiments, such as human cell line activation tests, still have shortcomings such as time-consuming and high cost in the research of predicting chemical sensitization.

Method used

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  • Method for detecting sensitization of cosmetics components by ultra-weak biophoton imaging system
  • Method for detecting sensitization of cosmetics components by ultra-weak biophoton imaging system
  • Method for detecting sensitization of cosmetics components by ultra-weak biophoton imaging system

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Embodiment

[0047] Example: Ultra-weak biophoton imaging system detects cosmetic allergies

[0048] 1. Experimental materials:

[0049] Human skin tissue, from the Chinese Brain Bank Center (CBBC)

[0050] 2. Experimental steps

[0051] S1, the pretreatment of material, comprises the following steps:

[0052] S11. Retrieve the skin on the back of the forearm from the donated body provided by the Red Cross, and use a scalpel and a sharp blade to remove the subcutaneous fat in a large petri dish filled with a large amount of PBS buffer, leaving only the dermis and epidermis as much as possible .

[0053] S12. After removing the fat, cut the tissue into multiple 0.5×0.5cm tissue blocks, put them in antifreeze solution and infiltrate them in a -4°C refrigerator, and then divide the infiltrated tissue blocks into 2.0ml decanters Spin round bottom cryovials and store in liquid nitrogen at -80°C.

[0054] S2, tissue block culture, comprising the following steps:

[0055] S21. Take out the ...

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Abstract

The invention discloses a method for detecting sensitization of cosmetics components by AN ultra-weak biophoton imaging system, and the method includes the following steps: S1, material pretreatment;S2, tissue block culture; S3, experimental group setting; S4, experimental preparation and imaging system preheating; S5, positioning photography; S6, experimental imaging; and S7, sequence image processing and data analysis. After a cultured skin tissue donated by a dead body is treated with common cosmetic sensitizer DNCB (Dinitrochlorobenzene) by an ultraweak biophoton imaging technology and animmunohistochemical staining method, and changes of biophoton radiation intensity can be detected for fast detection of skin sensitizers in cosmetics. The method can quickly accurately explore the sensitization of the cosmetics components to detect whether chemical ingredients causing skin allergic injury are contained in the cosmetics, and at the same time, the method has the characteristics ofsimple and fast operation.

Description

technical field [0001] The invention relates to the safety detection of cosmetics. An ultra-weak biophoton imaging system is used to detect the change of biophoton intensity of spontaneous radiation of human skin treated by cosmetics, so as to determine whether the cosmetics contain chemical components that can cause skin allergic damage. It belongs to the field of biomedical detection technology, and this technology can also be applied to the fields of life science research, medical clinical examination and environmental protection. Background technique [0002] The composition of most cosmetics is complex, and the common allergens in the ingredients include fragrances, flavors, preservatives, metal elements (lead, mercury, nickel, arsenic), surfactants, emulsifiers, hormones (corticosteroid hormones, estrogens) etc.), moisturizers, 1-chloro-2,4-dinitrobenzene (DNCB), natural plant ingredients, etc. The diversification and high usage rate of cosmetics have led to an increa...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N1/28G01N1/42
CPCG01N1/286G01N1/42G01N21/84G01N2001/2873
Inventor 戴甲培郑敏明李泽华肖芳艳孙燕
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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