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Method for separating and purifying schizophyllan by two aqueous phase extraction

A technology for separation and purification of schizophyllan, applied in the field of separation and purification, to achieve good emulsification, product quality improvement, and structural integrity

Inactive Publication Date: 2018-10-19
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aqueous two-phase extraction technology has attracted wide attention in the field of biochemical industry. It is a separation technology that utilizes the difference in the distribution coefficient of solutes between two phases. It has the advantages of simple operation and easy scale-up. The method of phylloidin has not been reported yet

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  • Method for separating and purifying schizophyllan by two aqueous phase extraction
  • Method for separating and purifying schizophyllan by two aqueous phase extraction
  • Method for separating and purifying schizophyllan by two aqueous phase extraction

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1, the influence of process parameter change on polysaccharide extraction rate and protein clearance rate

[0031] 1.1 Purpose of the test

[0032] On the basis of Example 1, the influence of different solid-liquid ratios and the mass fractions of sodium carbonate and absolute ethanol in the two-phase extraction on the extraction rate of polysaccharides and the clearance rate of proteins

[0033] 1.2 Test method

[0034] 12.1 Determination of the concentration of schizophyllan: use the sulfuric acid-phenol method to dilute the sample with a certain multiple, take 50 μL of each diluted sample solution and add it to a 96-well plate, then add 25 μL of 6% phenol solution and 125 μL of concentrated sulfuric acid, and let it stand for 30 minutes Afterwards, the absorbance was measured at 490 nm with a microplate reader. At the same time, glucose was used as a reference substance to make a standard curve, and the concentration of schizophyllan was calculated accor...

Embodiment 2

[0041] Example 2. A method for separating and purifying schizophyllan by aqueous two-phase extraction

[0042] A) Weigh sodium carbonate, add appropriate amount of water, after stirring and dissolving, add dehydrated alcohol, fully mix, make the massfraction of dehydrated alcohol in the two-phase system be 25%, the massfraction of sodium carbonate be 20%, establish Ethanol / sodium carbonate two-phase aqueous system;

[0043] B) Take the purchased Schizophyllum fermented liquid and add 1 times its weight of deionized water to dilute, remove most of the thallus through a plate and frame filter, and the working pressure is 0.20Mpa; further remove the thalline by a high-speed tubular centrifuge, 20000rpm, according to the ratio of material to liquid is 1:5, add Schizophyllum fermented liquid into the above-mentioned two-phase system, use an electric stirring device at 200rpm, stir for 1min, fully mix and let stand for 5min, until the solution is completely phase-formed, then Use a...

Embodiment 3

[0045] Example 3. A method for separating and purifying schizophyllan by aqueous two-phase extraction

[0046] A) Weigh sodium carbonate, add appropriate amount of water, after stirring and dissolving, add dehydrated alcohol, fully mix, make the massfraction of dehydrated alcohol in the two-phase system be 15%, the massfraction of sodium carbonate be 10%, establish Ethanol / sodium carbonate two-phase aqueous system;

[0047]B) Take the purchased Schizophyllum fermented liquid and add 1 times the weight of deionized water to dilute it, remove most of the thalline through a plate and frame filter, and the working pressure is 0.18Mpa; further remove the thalline by a high-speed tubular centrifuge, 10000rpm, according to the ratio of material to liquid is 1:10, add Schizophyllum fermented liquid into the above-mentioned two-phase system, use an electric stirring device at 200rpm, stir for 1min, fully mix and let stand for 5min, until the solution is completely phase-formed, then U...

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Abstract

The invention relates to the technical field of schizophyllan extraction, and discloses a method for separating and purifying schizophyllan by two aqueous phase extraction. The method comprises the following steps of preparing schizophyllum fermentation solution and performing extraction by using a sodium carbonate / ethyl alcohol dual water phase extraction system. The schizophyllan extracted by the method has the advantages that the structure is complete; the schizophyllan yield is high; the protein removal rate is high; the operation is simple and convenient; the toxicity is low; the safety is realized and the industrial scale preparation is easy.

Description

technical field [0001] The invention relates to the technical field of separation and purification, in particular to a method for separating and purifying schizophyllan by means of two-phase extraction. Background technique [0002] Schizophyllum, also known as white ginseng, tree flower, cockscomb, chicken feather fungus, etc., is a large fungus with high nutritional and medicinal value. Schizophyllum polysaccharide (SPG) is a water-soluble polysaccharide extracted from the fruiting body, mycelium or fermentation broth of Schizophyllum fungus. The main chain is β-(1→3)D glucan structure, with β-(1→ 6) Glycosidic side chains. SPG has a variety of physiological activities such as tumor inhibition, antibacterial and anti-inflammatory, anti-radiation, and improvement of body immunity. The natural conformation of SPG in aqueous solution has a triple helix structure, and if the triple helix structure is destroyed, its antitumor activity will be affected. [0003] The conventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
CPCC08B37/0003C08B37/0024
Inventor 周林余菁菁吴继宏朱爽黄嘉欣
Owner GUANGDONG PHARMA UNIV
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