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Application of teriflunomide to preparation of anti-west Nile virus medicine

A technology of West Nile virus and teriflunomide, applied in the field of medicine, can solve problems such as high infection, and achieve the effect of improving survival rate and low toxicity

Inactive Publication Date: 2018-10-19
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

West Nile virus is a highly infectious and highly pathogenic pathogen, and research on West Nile virus can only be carried out in biosafety level 3 laboratories

Method used

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  • Application of teriflunomide to preparation of anti-west Nile virus medicine
  • Application of teriflunomide to preparation of anti-west Nile virus medicine
  • Application of teriflunomide to preparation of anti-west Nile virus medicine

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Embodiment 1

[0029] 1. Experimental drugs, reagents and materials

[0030] 1. Compounds: 978 US FDA-approved compounds were purchased from Selleck, US.

[0031] 2. Monkey kidney cell line Vero and human liver cancer cell line Huh7 were purchased from Shanghai Institute of Cell Research, Chinese Academy of Sciences, and preserved by the Department of Microbiology, Department of Naval Medicine, Second Military Medical University, Chinese People's Liberation Army.

[0032] 3. Complete cell culture medium of DMEM cells, containing 10% fetal bovine serum, 0.03% glutamine, non-essential amino acids, ampicillin and streptomycin 100U / ml, adjusted to pH 7.4.

[0033] 4. Cell digestion solution, containing 0.25% trypsin, prepared with phosphate buffer.

[0034] 5. West Nile virus NS1 antibody was purchased from abcam company in the United Kingdom.

[0035] 6. CellTiter-Glo luminescence cell viability detection kit is a product of American Promega Company.

[0036] 7. Carboxymethylcellulose sodium...

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Abstract

The invention discloses application of teriflunomide to preparation of an anti-west Nile virus medicine. The anti-west Nile virus medicine takes the teriflunomide as a single active component or is amedicine composition containing the teriflunomide; and the anti-west Nile virus medicine is a medicine for preventing or treating west Nile virus infection. A susceptible cell line containing the westNile virus comprises a human liver cancer cell line Huh7, a monkey kidney cell Vero and a west Nile virus infected mouse model, and is used for detecting the anti-west Nile virus activity of the teriflunomide. The experimental result shows that the teriflunomide can effectively inhibit infection on the above susceptible cell and the animal model by the west Nile virus as well as proves that the teriflunomide can effectively inhibit west Nile virus from infecting a targeted cell, can increase the survival rate of the west Nile virus infected mice, has low toxicity, can serve as a potential anti-west Nile virus medicine and has further development prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and particularly relates to the application of teriflunomide in the preparation of an anti-West Nile virus drug. Background technique [0002] Terilunomide is an FDA-approved drug used as an immunosuppressant for organ transplantation, and there is no literature reporting its effect on West Nile virus. [0003] The chemical structural formula of teriflunomide is as follows (Jiwon Oh and Paul W O’Connor. An update of teriflunomide for treatment of multiple sclerosis. Ther Clin Risk Manag, 2013, 9.177-190): [0004] [0005] West Nile virus (WNV) is transmitted through insect vectors, severe patients can develop encephalitis, and the mortality rate is about 1%. Currently, there is no effective treatment drug. West Nile virus is a highly infectious and highly pathogenic pathogen, and West Nile virus research can only be carried out in biosafety level 3 laboratories. [0006] In view of this, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/277A61P31/14
CPCA61K31/277A61P31/14Y02A50/30
Inventor 唐海琳赵平彭浩然刘阳任浩何燕华戚中田
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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